Job ID = 9161782 sra ファイルのダウンロード中... Completed: 645368K bytes transferred in 11 seconds (462197K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Written 15463981 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1044171/SRR2045652.sra Written 15463981 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:16 15463981 reads; of these: 15463981 (100.00%) were paired; of these: 6975758 (45.11%) aligned concordantly 0 times 7758757 (50.17%) aligned concordantly exactly 1 time 729466 (4.72%) aligned concordantly >1 times ---- 6975758 pairs aligned concordantly 0 times; of these: 365865 (5.24%) aligned discordantly 1 time ---- 6609893 pairs aligned 0 times concordantly or discordantly; of these: 13219786 mates make up the pairs; of these: 13004827 (98.37%) aligned 0 times 128877 (0.97%) aligned exactly 1 time 86082 (0.65%) aligned >1 times 57.95% overall alignment rate Time searching: 00:07:16 Overall time: 00:07:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5202128 / 8807779 = 0.5906 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:45:16: # Command line: callpeak -t SRX1044171.bam -f BAM -g 12100000 -n SRX1044171.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1044171.20 # format = BAM # ChIP-seq file = ['SRX1044171.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:45:16: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:45:16: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:45:16: # Command line: callpeak -t SRX1044171.bam -f BAM -g 12100000 -n SRX1044171.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1044171.05 # format = BAM # ChIP-seq file = ['SRX1044171.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:45:16: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:45:16: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:45:16: # Command line: callpeak -t SRX1044171.bam -f BAM -g 12100000 -n SRX1044171.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1044171.10 # format = BAM # ChIP-seq file = ['SRX1044171.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:45:16: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:45:16: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:45:22: 1000000 INFO @ Wed, 28 Jun 2017 04:45:22: 1000000 INFO @ Wed, 28 Jun 2017 04:45:22: 1000000 INFO @ Wed, 28 Jun 2017 04:45:28: 2000000 INFO @ Wed, 28 Jun 2017 04:45:29: 2000000 INFO @ Wed, 28 Jun 2017 04:45:29: 2000000 INFO @ Wed, 28 Jun 2017 04:45:35: 3000000 INFO @ Wed, 28 Jun 2017 04:45:35: 3000000 INFO @ Wed, 28 Jun 2017 04:45:35: 3000000 INFO @ Wed, 28 Jun 2017 04:45:41: 4000000 INFO @ Wed, 28 Jun 2017 04:45:41: 4000000 INFO @ Wed, 28 Jun 2017 04:45:41: 4000000 INFO @ Wed, 28 Jun 2017 04:45:47: 5000000 INFO @ Wed, 28 Jun 2017 04:45:47: 5000000 INFO @ Wed, 28 Jun 2017 04:45:48: 5000000 INFO @ Wed, 28 Jun 2017 04:45:53: 6000000 INFO @ Wed, 28 Jun 2017 04:45:54: 6000000 INFO @ Wed, 28 Jun 2017 04:45:54: 6000000 INFO @ Wed, 28 Jun 2017 04:45:59: 7000000 INFO @ Wed, 28 Jun 2017 04:46:00: 7000000 INFO @ Wed, 28 Jun 2017 04:46:00: 7000000 INFO @ Wed, 28 Jun 2017 04:46:03: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 04:46:03: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 04:46:03: #1 total tags in treatment: 3439286 INFO @ Wed, 28 Jun 2017 04:46:03: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:46:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:46:03: #1 tags after filtering in treatment: 2834239 INFO @ Wed, 28 Jun 2017 04:46:03: #1 Redundant rate of treatment: 0.18 INFO @ Wed, 28 Jun 2017 04:46:03: #1 finished! INFO @ Wed, 28 Jun 2017 04:46:03: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:46:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:46:03: #2 number of paired peaks: 143 WARNING @ Wed, 28 Jun 2017 04:46:03: Fewer paired peaks (143) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 143 pairs to build model! INFO @ Wed, 28 Jun 2017 04:46:03: start model_add_line... INFO @ Wed, 28 Jun 2017 04:46:03: start X-correlation... INFO @ Wed, 28 Jun 2017 04:46:03: end of X-cor INFO @ Wed, 28 Jun 2017 04:46:03: #2 finished! INFO @ Wed, 28 Jun 2017 04:46:03: #2 predicted fragment length is 133 bps INFO @ Wed, 28 Jun 2017 04:46:03: #2 alternative fragment length(s) may be 4,117,133,150 bps INFO @ Wed, 28 Jun 2017 04:46:03: #2.2 Generate R script for model : SRX1044171.10_model.r INFO @ Wed, 28 Jun 2017 04:46:03: #3 Call peaks... INFO @ Wed, 28 Jun 2017 04:46:03: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 28 Jun 2017 04:46:03: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 04:46:03: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 04:46:03: #1 total tags in treatment: 3439286 INFO @ Wed, 28 Jun 2017 04:46:03: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:46:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:46:03: #1 tags after filtering in treatment: 2834239 INFO @ Wed, 28 Jun 2017 04:46:03: #1 Redundant rate of treatment: 0.18 INFO @ Wed, 28 Jun 2017 04:46:03: #1 finished! INFO @ Wed, 28 Jun 2017 04:46:03: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:46:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:46:03: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 04:46:03: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 04:46:03: #1 total tags in treatment: 3439286 INFO @ Wed, 28 Jun 2017 04:46:03: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:46:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:46:03: #1 tags after filtering in treatment: 2834239 INFO @ Wed, 28 Jun 2017 04:46:03: #1 Redundant rate of treatment: 0.18 INFO @ Wed, 28 Jun 2017 04:46:03: #1 finished! INFO @ Wed, 28 Jun 2017 04:46:03: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:46:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:46:03: #2 number of paired peaks: 143 WARNING @ Wed, 28 Jun 2017 04:46:03: Fewer paired peaks (143) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 143 pairs to build model! INFO @ Wed, 28 Jun 2017 04:46:03: start model_add_line... INFO @ Wed, 28 Jun 2017 04:46:03: start X-correlation... INFO @ Wed, 28 Jun 2017 04:46:03: end of X-cor INFO @ Wed, 28 Jun 2017 04:46:03: #2 finished! INFO @ Wed, 28 Jun 2017 04:46:03: #2 predicted fragment length is 133 bps INFO @ Wed, 28 Jun 2017 04:46:03: #2 alternative fragment length(s) may be 4,117,133,150 bps INFO @ Wed, 28 Jun 2017 04:46:03: #2.2 Generate R script for model : SRX1044171.05_model.r INFO @ Wed, 28 Jun 2017 04:46:03: #3 Call peaks... INFO @ Wed, 28 Jun 2017 04:46:03: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 28 Jun 2017 04:46:03: #2 number of paired peaks: 143 WARNING @ Wed, 28 Jun 2017 04:46:03: Fewer paired peaks (143) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 143 pairs to build model! INFO @ Wed, 28 Jun 2017 04:46:03: start model_add_line... INFO @ Wed, 28 Jun 2017 04:46:03: start X-correlation... INFO @ Wed, 28 Jun 2017 04:46:03: end of X-cor INFO @ Wed, 28 Jun 2017 04:46:03: #2 finished! INFO @ Wed, 28 Jun 2017 04:46:03: #2 predicted fragment length is 133 bps INFO @ Wed, 28 Jun 2017 04:46:03: #2 alternative fragment length(s) may be 4,117,133,150 bps INFO @ Wed, 28 Jun 2017 04:46:03: #2.2 Generate R script for model : SRX1044171.20_model.r INFO @ Wed, 28 Jun 2017 04:46:03: #3 Call peaks... INFO @ Wed, 28 Jun 2017 04:46:03: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 28 Jun 2017 04:46:11: #3 Call peaks for each chromosome... INFO @ Wed, 28 Jun 2017 04:46:11: #3 Call peaks for each chromosome... INFO @ Wed, 28 Jun 2017 04:46:12: #3 Call peaks for each chromosome... INFO @ Wed, 28 Jun 2017 04:46:14: #4 Write output xls file... SRX1044171.10_peaks.xls INFO @ Wed, 28 Jun 2017 04:46:14: #4 Write peak in narrowPeak format file... SRX1044171.10_peaks.narrowPeak INFO @ Wed, 28 Jun 2017 04:46:14: #4 Write summits bed file... SRX1044171.10_summits.bed INFO @ Wed, 28 Jun 2017 04:46:14: Done! pass1 - making usageList (16 chroms): 0 millis pass2 - checking and writing primary data (526 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:46:15: #4 Write output xls file... SRX1044171.05_peaks.xls INFO @ Wed, 28 Jun 2017 04:46:15: #4 Write peak in narrowPeak format file... SRX1044171.05_peaks.narrowPeak INFO @ Wed, 28 Jun 2017 04:46:15: #4 Write summits bed file... SRX1044171.05_summits.bed INFO @ Wed, 28 Jun 2017 04:46:15: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (856 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:46:15: #4 Write output xls file... SRX1044171.20_peaks.xls INFO @ Wed, 28 Jun 2017 04:46:15: #4 Write peak in narrowPeak format file... SRX1044171.20_peaks.narrowPeak INFO @ Wed, 28 Jun 2017 04:46:15: #4 Write summits bed file... SRX1044171.20_summits.bed INFO @ Wed, 28 Jun 2017 04:46:15: Done! pass1 - making usageList (16 chroms): 0 millis pass2 - checking and writing primary data (322 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。