Job ID = 14519951 SRX = SRX10341705 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25040499 spots for SRR13963713/SRR13963713.sra Written 25040499 spots for SRR13963713/SRR13963713.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:04 25040499 reads; of these: 25040499 (100.00%) were unpaired; of these: 2708880 (10.82%) aligned 0 times 18851695 (75.28%) aligned exactly 1 time 3479924 (13.90%) aligned >1 times 89.18% overall alignment rate Time searching: 00:10:04 Overall time: 00:10:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11662074 / 22331619 = 0.5222 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:31:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:31:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:31:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:31:39: 1000000 INFO @ Sat, 15 Jan 2022 18:31:48: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:31:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:31:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:31:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:31:59: 3000000 INFO @ Sat, 15 Jan 2022 18:32:09: 1000000 INFO @ Sat, 15 Jan 2022 18:32:09: 4000000 INFO @ Sat, 15 Jan 2022 18:32:19: 2000000 INFO @ Sat, 15 Jan 2022 18:32:20: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:32:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:32:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:32:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:32:29: 6000000 INFO @ Sat, 15 Jan 2022 18:32:30: 3000000 INFO @ Sat, 15 Jan 2022 18:32:38: 7000000 INFO @ Sat, 15 Jan 2022 18:32:39: 1000000 INFO @ Sat, 15 Jan 2022 18:32:40: 4000000 INFO @ Sat, 15 Jan 2022 18:32:47: 8000000 INFO @ Sat, 15 Jan 2022 18:32:47: 2000000 INFO @ Sat, 15 Jan 2022 18:32:51: 5000000 INFO @ Sat, 15 Jan 2022 18:32:56: 3000000 INFO @ Sat, 15 Jan 2022 18:32:56: 9000000 INFO @ Sat, 15 Jan 2022 18:33:02: 6000000 INFO @ Sat, 15 Jan 2022 18:33:05: 4000000 INFO @ Sat, 15 Jan 2022 18:33:06: 10000000 INFO @ Sat, 15 Jan 2022 18:33:12: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 18:33:12: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 18:33:12: #1 total tags in treatment: 10669545 INFO @ Sat, 15 Jan 2022 18:33:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:33:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:33:13: 7000000 INFO @ Sat, 15 Jan 2022 18:33:13: #1 tags after filtering in treatment: 10669545 INFO @ Sat, 15 Jan 2022 18:33:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:33:13: #1 finished! INFO @ Sat, 15 Jan 2022 18:33:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:33:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:33:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:33:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:33:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:33:15: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:33:23: 8000000 INFO @ Sat, 15 Jan 2022 18:33:24: 6000000 INFO @ Sat, 15 Jan 2022 18:33:32: 9000000 INFO @ Sat, 15 Jan 2022 18:33:33: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:33:42: 10000000 INFO @ Sat, 15 Jan 2022 18:33:42: 8000000 INFO @ Sat, 15 Jan 2022 18:33:48: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 18:33:48: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 18:33:48: #1 total tags in treatment: 10669545 INFO @ Sat, 15 Jan 2022 18:33:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:33:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:33:48: #1 tags after filtering in treatment: 10669545 INFO @ Sat, 15 Jan 2022 18:33:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:33:48: #1 finished! INFO @ Sat, 15 Jan 2022 18:33:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:33:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:33:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:33:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:33:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:33:52: 9000000 INFO @ Sat, 15 Jan 2022 18:34:01: 10000000 INFO @ Sat, 15 Jan 2022 18:34:07: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 18:34:07: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 18:34:07: #1 total tags in treatment: 10669545 INFO @ Sat, 15 Jan 2022 18:34:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:34:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:34:07: #1 tags after filtering in treatment: 10669545 INFO @ Sat, 15 Jan 2022 18:34:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:34:07: #1 finished! INFO @ Sat, 15 Jan 2022 18:34:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:34:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:34:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:34:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:34:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341705/SRX10341705.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling