Job ID = 14519940 SRX = SRX10341700 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25324452 spots for SRR13963708/SRR13963708.sra Written 25324452 spots for SRR13963708/SRR13963708.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:45 25324452 reads; of these: 25324452 (100.00%) were unpaired; of these: 821674 (3.24%) aligned 0 times 21210153 (83.75%) aligned exactly 1 time 3292625 (13.00%) aligned >1 times 96.76% overall alignment rate Time searching: 00:03:45 Overall time: 00:03:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10917131 / 24502778 = 0.4455 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:17:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:17:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:17:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:17:50: 1000000 INFO @ Sat, 15 Jan 2022 18:17:56: 2000000 INFO @ Sat, 15 Jan 2022 18:18:02: 3000000 INFO @ Sat, 15 Jan 2022 18:18:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:18:13: 5000000 INFO @ Sat, 15 Jan 2022 18:18:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:18:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:18:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:18:20: 6000000 INFO @ Sat, 15 Jan 2022 18:18:21: 1000000 INFO @ Sat, 15 Jan 2022 18:18:27: 7000000 INFO @ Sat, 15 Jan 2022 18:18:27: 2000000 INFO @ Sat, 15 Jan 2022 18:18:33: 8000000 INFO @ Sat, 15 Jan 2022 18:18:33: 3000000 INFO @ Sat, 15 Jan 2022 18:18:39: 9000000 INFO @ Sat, 15 Jan 2022 18:18:40: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:18:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:18:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:18:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:18:46: 10000000 INFO @ Sat, 15 Jan 2022 18:18:46: 5000000 INFO @ Sat, 15 Jan 2022 18:18:50: 1000000 INFO @ Sat, 15 Jan 2022 18:18:52: 11000000 INFO @ Sat, 15 Jan 2022 18:18:53: 6000000 INFO @ Sat, 15 Jan 2022 18:18:57: 2000000 INFO @ Sat, 15 Jan 2022 18:18:58: 12000000 INFO @ Sat, 15 Jan 2022 18:18:59: 7000000 INFO @ Sat, 15 Jan 2022 18:19:03: 3000000 INFO @ Sat, 15 Jan 2022 18:19:05: 13000000 INFO @ Sat, 15 Jan 2022 18:19:05: 8000000 INFO @ Sat, 15 Jan 2022 18:19:09: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 18:19:09: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 18:19:09: #1 total tags in treatment: 13585647 INFO @ Sat, 15 Jan 2022 18:19:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:19:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:19:09: #1 tags after filtering in treatment: 13585647 INFO @ Sat, 15 Jan 2022 18:19:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:19:09: #1 finished! INFO @ Sat, 15 Jan 2022 18:19:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:19:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:19:09: 4000000 INFO @ Sat, 15 Jan 2022 18:19:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:19:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:19:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:19:12: 9000000 INFO @ Sat, 15 Jan 2022 18:19:16: 5000000 INFO @ Sat, 15 Jan 2022 18:19:18: 10000000 INFO @ Sat, 15 Jan 2022 18:19:23: 6000000 INFO @ Sat, 15 Jan 2022 18:19:25: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:19:29: 7000000 INFO @ Sat, 15 Jan 2022 18:19:31: 12000000 INFO @ Sat, 15 Jan 2022 18:19:36: 8000000 INFO @ Sat, 15 Jan 2022 18:19:38: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:19:42: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 18:19:42: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 18:19:42: #1 total tags in treatment: 13585647 INFO @ Sat, 15 Jan 2022 18:19:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:19:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:19:42: 9000000 INFO @ Sat, 15 Jan 2022 18:19:42: #1 tags after filtering in treatment: 13585647 INFO @ Sat, 15 Jan 2022 18:19:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:19:42: #1 finished! INFO @ Sat, 15 Jan 2022 18:19:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:19:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:19:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:19:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:19:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:19:48: 10000000 INFO @ Sat, 15 Jan 2022 18:19:54: 11000000 INFO @ Sat, 15 Jan 2022 18:20:00: 12000000 INFO @ Sat, 15 Jan 2022 18:20:05: 13000000 INFO @ Sat, 15 Jan 2022 18:20:08: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 18:20:08: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 18:20:08: #1 total tags in treatment: 13585647 INFO @ Sat, 15 Jan 2022 18:20:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:20:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:20:09: #1 tags after filtering in treatment: 13585647 INFO @ Sat, 15 Jan 2022 18:20:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:20:09: #1 finished! INFO @ Sat, 15 Jan 2022 18:20:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:20:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:20:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:20:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:20:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341700/SRX10341700.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling