Job ID = 14520149 SRX = SRX10341699 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 26089990 spots for SRR13963707/SRR13963707.sra Written 26089990 spots for SRR13963707/SRR13963707.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:31 26089990 reads; of these: 26089990 (100.00%) were unpaired; of these: 1847140 (7.08%) aligned 0 times 21636321 (82.93%) aligned exactly 1 time 2606529 (9.99%) aligned >1 times 92.92% overall alignment rate Time searching: 00:03:31 Overall time: 00:03:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12951409 / 24242850 = 0.5342 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:39:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:39:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:39:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:39:55: 1000000 INFO @ Sat, 15 Jan 2022 18:40:02: 2000000 INFO @ Sat, 15 Jan 2022 18:40:08: 3000000 INFO @ Sat, 15 Jan 2022 18:40:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:40:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:40:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:40:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:40:22: 5000000 INFO @ Sat, 15 Jan 2022 18:40:24: 1000000 INFO @ Sat, 15 Jan 2022 18:40:29: 6000000 INFO @ Sat, 15 Jan 2022 18:40:31: 2000000 INFO @ Sat, 15 Jan 2022 18:40:36: 7000000 INFO @ Sat, 15 Jan 2022 18:40:38: 3000000 INFO @ Sat, 15 Jan 2022 18:40:44: 8000000 INFO @ Sat, 15 Jan 2022 18:40:45: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:40:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:40:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:40:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:40:51: 9000000 INFO @ Sat, 15 Jan 2022 18:40:52: 5000000 INFO @ Sat, 15 Jan 2022 18:40:55: 1000000 INFO @ Sat, 15 Jan 2022 18:40:59: 10000000 INFO @ Sat, 15 Jan 2022 18:40:59: 6000000 INFO @ Sat, 15 Jan 2022 18:41:02: 2000000 INFO @ Sat, 15 Jan 2022 18:41:05: 7000000 INFO @ Sat, 15 Jan 2022 18:41:06: 11000000 INFO @ Sat, 15 Jan 2022 18:41:08: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:41:08: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:41:08: #1 total tags in treatment: 11291441 INFO @ Sat, 15 Jan 2022 18:41:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:41:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:41:08: #1 tags after filtering in treatment: 11291441 INFO @ Sat, 15 Jan 2022 18:41:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:41:08: #1 finished! INFO @ Sat, 15 Jan 2022 18:41:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:41:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:41:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:41:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:41:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 18:41:09: 3000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:41:12: 8000000 INFO @ Sat, 15 Jan 2022 18:41:15: 4000000 INFO @ Sat, 15 Jan 2022 18:41:19: 9000000 INFO @ Sat, 15 Jan 2022 18:41:22: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:41:25: 10000000 INFO @ Sat, 15 Jan 2022 18:41:28: 6000000 INFO @ Sat, 15 Jan 2022 18:41:32: 11000000 INFO @ Sat, 15 Jan 2022 18:41:34: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:41:34: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:41:34: #1 total tags in treatment: 11291441 INFO @ Sat, 15 Jan 2022 18:41:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:41:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:41:34: #1 tags after filtering in treatment: 11291441 INFO @ Sat, 15 Jan 2022 18:41:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:41:34: #1 finished! INFO @ Sat, 15 Jan 2022 18:41:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:41:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:41:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:41:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:41:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:41:35: 7000000 INFO @ Sat, 15 Jan 2022 18:41:40: 8000000 INFO @ Sat, 15 Jan 2022 18:41:46: 9000000 INFO @ Sat, 15 Jan 2022 18:41:53: 10000000 INFO @ Sat, 15 Jan 2022 18:41:59: 11000000 INFO @ Sat, 15 Jan 2022 18:42:00: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:42:00: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:42:00: #1 total tags in treatment: 11291441 INFO @ Sat, 15 Jan 2022 18:42:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:42:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:42:00: #1 tags after filtering in treatment: 11291441 INFO @ Sat, 15 Jan 2022 18:42:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:42:00: #1 finished! INFO @ Sat, 15 Jan 2022 18:42:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:42:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:42:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:42:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:42:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341699/SRX10341699.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling