Job ID = 14520148 SRX = SRX10341698 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25890132 spots for SRR13963706/SRR13963706.sra Written 25890132 spots for SRR13963706/SRR13963706.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:43 25890132 reads; of these: 25890132 (100.00%) were unpaired; of these: 595264 (2.30%) aligned 0 times 21358561 (82.50%) aligned exactly 1 time 3936307 (15.20%) aligned >1 times 97.70% overall alignment rate Time searching: 00:03:43 Overall time: 00:03:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11319408 / 25294868 = 0.4475 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:39:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:39:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:39:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:39:50: 1000000 INFO @ Sat, 15 Jan 2022 18:39:56: 2000000 INFO @ Sat, 15 Jan 2022 18:40:01: 3000000 INFO @ Sat, 15 Jan 2022 18:40:06: 4000000 INFO @ Sat, 15 Jan 2022 18:40:12: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:40:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:40:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:40:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:40:17: 6000000 INFO @ Sat, 15 Jan 2022 18:40:21: 1000000 INFO @ Sat, 15 Jan 2022 18:40:23: 7000000 INFO @ Sat, 15 Jan 2022 18:40:27: 2000000 INFO @ Sat, 15 Jan 2022 18:40:29: 8000000 INFO @ Sat, 15 Jan 2022 18:40:33: 3000000 INFO @ Sat, 15 Jan 2022 18:40:35: 9000000 INFO @ Sat, 15 Jan 2022 18:40:39: 4000000 INFO @ Sat, 15 Jan 2022 18:40:41: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:40:45: 5000000 INFO @ Sat, 15 Jan 2022 18:40:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:40:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:40:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:40:47: 11000000 INFO @ Sat, 15 Jan 2022 18:40:51: 6000000 INFO @ Sat, 15 Jan 2022 18:40:52: 1000000 INFO @ Sat, 15 Jan 2022 18:40:53: 12000000 INFO @ Sat, 15 Jan 2022 18:40:57: 7000000 INFO @ Sat, 15 Jan 2022 18:40:59: 13000000 INFO @ Sat, 15 Jan 2022 18:41:00: 2000000 INFO @ Sat, 15 Jan 2022 18:41:03: 8000000 INFO @ Sat, 15 Jan 2022 18:41:05: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:41:05: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:41:05: #1 total tags in treatment: 13975460 INFO @ Sat, 15 Jan 2022 18:41:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:41:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:41:05: #1 tags after filtering in treatment: 13975460 INFO @ Sat, 15 Jan 2022 18:41:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:41:05: #1 finished! INFO @ Sat, 15 Jan 2022 18:41:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:41:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:41:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:41:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:41:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:41:07: 3000000 INFO @ Sat, 15 Jan 2022 18:41:09: 9000000 INFO @ Sat, 15 Jan 2022 18:41:14: 4000000 INFO @ Sat, 15 Jan 2022 18:41:16: 10000000 INFO @ Sat, 15 Jan 2022 18:41:21: 5000000 INFO @ Sat, 15 Jan 2022 18:41:22: 11000000 INFO @ Sat, 15 Jan 2022 18:41:28: 12000000 INFO @ Sat, 15 Jan 2022 18:41:29: 6000000 INFO @ Sat, 15 Jan 2022 18:41:34: 13000000 INFO @ Sat, 15 Jan 2022 18:41:36: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:41:40: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:41:40: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:41:40: #1 total tags in treatment: 13975460 INFO @ Sat, 15 Jan 2022 18:41:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:41:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:41:40: #1 tags after filtering in treatment: 13975460 INFO @ Sat, 15 Jan 2022 18:41:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:41:40: #1 finished! INFO @ Sat, 15 Jan 2022 18:41:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:41:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:41:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:41:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:41:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:41:43: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:41:50: 9000000 INFO @ Sat, 15 Jan 2022 18:41:56: 10000000 INFO @ Sat, 15 Jan 2022 18:42:03: 11000000 INFO @ Sat, 15 Jan 2022 18:42:09: 12000000 INFO @ Sat, 15 Jan 2022 18:42:16: 13000000 INFO @ Sat, 15 Jan 2022 18:42:22: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:42:22: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:42:22: #1 total tags in treatment: 13975460 INFO @ Sat, 15 Jan 2022 18:42:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:42:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:42:22: #1 tags after filtering in treatment: 13975460 INFO @ Sat, 15 Jan 2022 18:42:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:42:22: #1 finished! INFO @ Sat, 15 Jan 2022 18:42:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:42:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:42:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:42:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:42:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10341698/SRX10341698.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling