Job ID = 9161752 sra ファイルのダウンロード中... Completed: 665122K bytes transferred in 9 seconds (554984K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20168393 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1018460/SRR2009027.sra Written 20168393 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:58 20168393 reads; of these: 20168393 (100.00%) were unpaired; of these: 488441 (2.42%) aligned 0 times 14341935 (71.11%) aligned exactly 1 time 5338017 (26.47%) aligned >1 times 97.58% overall alignment rate Time searching: 00:03:58 Overall time: 00:03:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9061510 / 19679952 = 0.4604 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:36:46: # Command line: callpeak -t SRX1018460.bam -f BAM -g 12100000 -n SRX1018460.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1018460.20 # format = BAM # ChIP-seq file = ['SRX1018460.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:36:46: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:36:46: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:36:46: # Command line: callpeak -t SRX1018460.bam -f BAM -g 12100000 -n SRX1018460.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1018460.05 # format = BAM # ChIP-seq file = ['SRX1018460.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:36:46: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:36:46: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:36:46: # Command line: callpeak -t SRX1018460.bam -f BAM -g 12100000 -n SRX1018460.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1018460.10 # format = BAM # ChIP-seq file = ['SRX1018460.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:36:46: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:36:46: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:36:54: 1000000 INFO @ Wed, 28 Jun 2017 04:36:54: 1000000 INFO @ Wed, 28 Jun 2017 04:36:54: 1000000 INFO @ Wed, 28 Jun 2017 04:37:02: 2000000 INFO @ Wed, 28 Jun 2017 04:37:02: 2000000 INFO @ Wed, 28 Jun 2017 04:37:02: 2000000 INFO @ Wed, 28 Jun 2017 04:37:09: 3000000 INFO @ Wed, 28 Jun 2017 04:37:09: 3000000 INFO @ Wed, 28 Jun 2017 04:37:10: 3000000 INFO @ Wed, 28 Jun 2017 04:37:17: 4000000 INFO @ Wed, 28 Jun 2017 04:37:17: 4000000 INFO @ Wed, 28 Jun 2017 04:37:17: 4000000 INFO @ Wed, 28 Jun 2017 04:37:24: 5000000 INFO @ Wed, 28 Jun 2017 04:37:24: 5000000 INFO @ Wed, 28 Jun 2017 04:37:24: 5000000 INFO @ Wed, 28 Jun 2017 04:37:31: 6000000 INFO @ Wed, 28 Jun 2017 04:37:32: 6000000 INFO @ Wed, 28 Jun 2017 04:37:32: 6000000 INFO @ Wed, 28 Jun 2017 04:37:39: 7000000 INFO @ Wed, 28 Jun 2017 04:37:39: 7000000 INFO @ Wed, 28 Jun 2017 04:37:40: 7000000 INFO @ Wed, 28 Jun 2017 04:37:46: 8000000 INFO @ Wed, 28 Jun 2017 04:37:47: 8000000 INFO @ Wed, 28 Jun 2017 04:37:47: 8000000 INFO @ Wed, 28 Jun 2017 04:37:53: 9000000 INFO @ Wed, 28 Jun 2017 04:37:55: 9000000 INFO @ Wed, 28 Jun 2017 04:37:55: 9000000 INFO @ Wed, 28 Jun 2017 04:38:00: 10000000 INFO @ Wed, 28 Jun 2017 04:38:02: 10000000 INFO @ Wed, 28 Jun 2017 04:38:02: 10000000 INFO @ Wed, 28 Jun 2017 04:38:05: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:38:05: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:38:05: #1 total tags in treatment: 10618442 INFO @ Wed, 28 Jun 2017 04:38:05: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:38:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:38:05: #1 tags after filtering in treatment: 10618442 INFO @ Wed, 28 Jun 2017 04:38:05: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:38:05: #1 finished! INFO @ Wed, 28 Jun 2017 04:38:05: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:38:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:38:06: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:38:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:38:06: Process for pairing-model is terminated! cat: SRX1018460.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1018460.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018460.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018460.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:38:07: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:38:07: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:38:07: #1 total tags in treatment: 10618442 INFO @ Wed, 28 Jun 2017 04:38:07: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:38:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:38:07: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:38:07: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:38:07: #1 total tags in treatment: 10618442 INFO @ Wed, 28 Jun 2017 04:38:07: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:38:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:38:07: #1 tags after filtering in treatment: 10618442 INFO @ Wed, 28 Jun 2017 04:38:07: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:38:07: #1 finished! INFO @ Wed, 28 Jun 2017 04:38:07: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:38:07: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:38:07: #1 tags after filtering in treatment: 10618442 INFO @ Wed, 28 Jun 2017 04:38:07: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:38:07: #1 finished! INFO @ Wed, 28 Jun 2017 04:38:07: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:38:07: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:38:08: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:38:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:38:08: Process for pairing-model is terminated! cat: SRX1018460.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1018460.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018460.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018460.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:38:08: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:38:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:38:08: Process for pairing-model is terminated! cat: SRX1018460.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1018460.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018460.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018460.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。