Job ID = 9161751 sra ファイルのダウンロード中... Completed: 823921K bytes transferred in 10 seconds (673836K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 24937511 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1018459/SRR2009026.sra Written 24937511 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:19 24937511 reads; of these: 24937511 (100.00%) were unpaired; of these: 610982 (2.45%) aligned 0 times 18522425 (74.28%) aligned exactly 1 time 5804104 (23.27%) aligned >1 times 97.55% overall alignment rate Time searching: 00:04:19 Overall time: 00:04:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11833378 / 24326529 = 0.4864 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:38:03: # Command line: callpeak -t SRX1018459.bam -f BAM -g 12100000 -n SRX1018459.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1018459.20 # format = BAM # ChIP-seq file = ['SRX1018459.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:38:03: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:38:03: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:38:03: # Command line: callpeak -t SRX1018459.bam -f BAM -g 12100000 -n SRX1018459.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1018459.10 # format = BAM # ChIP-seq file = ['SRX1018459.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:38:03: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:38:03: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:38:03: # Command line: callpeak -t SRX1018459.bam -f BAM -g 12100000 -n SRX1018459.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1018459.05 # format = BAM # ChIP-seq file = ['SRX1018459.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:38:03: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:38:03: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:38:09: 1000000 INFO @ Wed, 28 Jun 2017 04:38:09: 1000000 INFO @ Wed, 28 Jun 2017 04:38:10: 1000000 INFO @ Wed, 28 Jun 2017 04:38:15: 2000000 INFO @ Wed, 28 Jun 2017 04:38:16: 2000000 INFO @ Wed, 28 Jun 2017 04:38:16: 2000000 INFO @ Wed, 28 Jun 2017 04:38:21: 3000000 INFO @ Wed, 28 Jun 2017 04:38:22: 3000000 INFO @ Wed, 28 Jun 2017 04:38:22: 3000000 INFO @ Wed, 28 Jun 2017 04:38:28: 4000000 INFO @ Wed, 28 Jun 2017 04:38:28: 4000000 INFO @ Wed, 28 Jun 2017 04:38:28: 4000000 INFO @ Wed, 28 Jun 2017 04:38:34: 5000000 INFO @ Wed, 28 Jun 2017 04:38:35: 5000000 INFO @ Wed, 28 Jun 2017 04:38:35: 5000000 INFO @ Wed, 28 Jun 2017 04:38:40: 6000000 INFO @ Wed, 28 Jun 2017 04:38:41: 6000000 INFO @ Wed, 28 Jun 2017 04:38:41: 6000000 INFO @ Wed, 28 Jun 2017 04:38:46: 7000000 INFO @ Wed, 28 Jun 2017 04:38:47: 7000000 INFO @ Wed, 28 Jun 2017 04:38:48: 7000000 INFO @ Wed, 28 Jun 2017 04:38:53: 8000000 INFO @ Wed, 28 Jun 2017 04:38:53: 8000000 INFO @ Wed, 28 Jun 2017 04:38:54: 8000000 INFO @ Wed, 28 Jun 2017 04:38:59: 9000000 INFO @ Wed, 28 Jun 2017 04:38:59: 9000000 INFO @ Wed, 28 Jun 2017 04:39:01: 9000000 INFO @ Wed, 28 Jun 2017 04:39:05: 10000000 INFO @ Wed, 28 Jun 2017 04:39:05: 10000000 INFO @ Wed, 28 Jun 2017 04:39:07: 10000000 INFO @ Wed, 28 Jun 2017 04:39:11: 11000000 INFO @ Wed, 28 Jun 2017 04:39:12: 11000000 INFO @ Wed, 28 Jun 2017 04:39:14: 11000000 INFO @ Wed, 28 Jun 2017 04:39:18: 12000000 INFO @ Wed, 28 Jun 2017 04:39:18: 12000000 INFO @ Wed, 28 Jun 2017 04:39:20: 12000000 INFO @ Wed, 28 Jun 2017 04:39:21: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:39:21: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:39:21: #1 total tags in treatment: 12493151 INFO @ Wed, 28 Jun 2017 04:39:21: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:39:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:39:21: #1 tags after filtering in treatment: 12493151 INFO @ Wed, 28 Jun 2017 04:39:21: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:39:21: #1 finished! INFO @ Wed, 28 Jun 2017 04:39:21: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:39:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:39:21: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:39:21: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:39:21: #1 total tags in treatment: 12493151 INFO @ Wed, 28 Jun 2017 04:39:21: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:39:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:39:21: #1 tags after filtering in treatment: 12493151 INFO @ Wed, 28 Jun 2017 04:39:21: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:39:21: #1 finished! INFO @ Wed, 28 Jun 2017 04:39:21: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:39:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:39:22: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:39:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:39:22: Process for pairing-model is terminated! cat: SRX1018459.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1018459.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018459.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018459.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:39:22: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:39:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:39:22: Process for pairing-model is terminated! cat: SRX1018459.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1018459.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018459.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018459.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:39:23: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:39:23: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:39:23: #1 total tags in treatment: 12493151 INFO @ Wed, 28 Jun 2017 04:39:23: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:39:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:39:23: #1 tags after filtering in treatment: 12493151 INFO @ Wed, 28 Jun 2017 04:39:23: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:39:23: #1 finished! INFO @ Wed, 28 Jun 2017 04:39:23: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:39:23: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:39:24: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:39:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:39:24: Process for pairing-model is terminated! cat: SRX1018459.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1018459.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018459.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1018459.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。