Job ID = 14520094 SRX = SRX10101475 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 9153975 spots for SRR13712787/SRR13712787.sra Written 9153975 spots for SRR13712787/SRR13712787.sra Read 9201024 spots for SRR13712788/SRR13712788.sra Written 9201024 spots for SRR13712788/SRR13712788.sra Read 9033180 spots for SRR13712789/SRR13712789.sra Written 9033180 spots for SRR13712789/SRR13712789.sra Read 22783713 spots for SRR13712790/SRR13712790.sra Written 22783713 spots for SRR13712790/SRR13712790.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:21 50171892 reads; of these: 50171892 (100.00%) were unpaired; of these: 1928232 (3.84%) aligned 0 times 39750596 (79.23%) aligned exactly 1 time 8493064 (16.93%) aligned >1 times 96.16% overall alignment rate Time searching: 00:07:21 Overall time: 00:07:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 30195630 / 48243660 = 0.6259 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:40:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:40:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:40:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:40:52: 1000000 INFO @ Sat, 15 Jan 2022 18:40:57: 2000000 INFO @ Sat, 15 Jan 2022 18:41:02: 3000000 INFO @ Sat, 15 Jan 2022 18:41:07: 4000000 INFO @ Sat, 15 Jan 2022 18:41:12: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:41:16: 6000000 INFO @ Sat, 15 Jan 2022 18:41:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:41:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:41:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:41:22: 7000000 INFO @ Sat, 15 Jan 2022 18:41:23: 1000000 INFO @ Sat, 15 Jan 2022 18:41:27: 8000000 INFO @ Sat, 15 Jan 2022 18:41:29: 2000000 INFO @ Sat, 15 Jan 2022 18:41:33: 9000000 INFO @ Sat, 15 Jan 2022 18:41:35: 3000000 INFO @ Sat, 15 Jan 2022 18:41:38: 10000000 INFO @ Sat, 15 Jan 2022 18:41:42: 4000000 INFO @ Sat, 15 Jan 2022 18:41:43: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:41:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:41:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:41:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:41:48: 5000000 INFO @ Sat, 15 Jan 2022 18:41:49: 12000000 INFO @ Sat, 15 Jan 2022 18:41:53: 1000000 INFO @ Sat, 15 Jan 2022 18:41:54: 6000000 INFO @ Sat, 15 Jan 2022 18:41:54: 13000000 INFO @ Sat, 15 Jan 2022 18:41:58: 2000000 INFO @ Sat, 15 Jan 2022 18:42:00: 14000000 INFO @ Sat, 15 Jan 2022 18:42:00: 7000000 INFO @ Sat, 15 Jan 2022 18:42:04: 3000000 INFO @ Sat, 15 Jan 2022 18:42:05: 15000000 INFO @ Sat, 15 Jan 2022 18:42:06: 8000000 INFO @ Sat, 15 Jan 2022 18:42:09: 4000000 INFO @ Sat, 15 Jan 2022 18:42:11: 16000000 INFO @ Sat, 15 Jan 2022 18:42:13: 9000000 INFO @ Sat, 15 Jan 2022 18:42:15: 5000000 INFO @ Sat, 15 Jan 2022 18:42:16: 17000000 INFO @ Sat, 15 Jan 2022 18:42:19: 10000000 INFO @ Sat, 15 Jan 2022 18:42:20: 6000000 INFO @ Sat, 15 Jan 2022 18:42:21: 18000000 INFO @ Sat, 15 Jan 2022 18:42:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:42:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:42:22: #1 total tags in treatment: 18048030 INFO @ Sat, 15 Jan 2022 18:42:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:42:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:42:22: #1 tags after filtering in treatment: 18048030 INFO @ Sat, 15 Jan 2022 18:42:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:42:22: #1 finished! INFO @ Sat, 15 Jan 2022 18:42:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:42:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:42:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:42:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:42:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:42:25: 11000000 INFO @ Sat, 15 Jan 2022 18:42:26: 7000000 INFO @ Sat, 15 Jan 2022 18:42:31: 12000000 INFO @ Sat, 15 Jan 2022 18:42:31: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:42:37: 9000000 INFO @ Sat, 15 Jan 2022 18:42:37: 13000000 INFO @ Sat, 15 Jan 2022 18:42:42: 10000000 INFO @ Sat, 15 Jan 2022 18:42:43: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:42:47: 11000000 INFO @ Sat, 15 Jan 2022 18:42:49: 15000000 INFO @ Sat, 15 Jan 2022 18:42:53: 12000000 INFO @ Sat, 15 Jan 2022 18:42:55: 16000000 INFO @ Sat, 15 Jan 2022 18:42:58: 13000000 INFO @ Sat, 15 Jan 2022 18:43:01: 17000000 INFO @ Sat, 15 Jan 2022 18:43:03: 14000000 INFO @ Sat, 15 Jan 2022 18:43:08: 18000000 INFO @ Sat, 15 Jan 2022 18:43:08: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:43:08: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:43:08: #1 total tags in treatment: 18048030 INFO @ Sat, 15 Jan 2022 18:43:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:43:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:43:08: #1 tags after filtering in treatment: 18048030 INFO @ Sat, 15 Jan 2022 18:43:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:43:08: #1 finished! INFO @ Sat, 15 Jan 2022 18:43:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:43:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:43:09: 15000000 INFO @ Sat, 15 Jan 2022 18:43:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:43:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:43:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:43:14: 16000000 INFO @ Sat, 15 Jan 2022 18:43:19: 17000000 INFO @ Sat, 15 Jan 2022 18:43:24: 18000000 INFO @ Sat, 15 Jan 2022 18:43:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:43:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:43:24: #1 total tags in treatment: 18048030 INFO @ Sat, 15 Jan 2022 18:43:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:43:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:43:24: #1 tags after filtering in treatment: 18048030 INFO @ Sat, 15 Jan 2022 18:43:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:43:24: #1 finished! INFO @ Sat, 15 Jan 2022 18:43:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:43:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:43:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:43:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:43:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101475/SRX10101475.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling