Job ID = 14520092 SRX = SRX10101474 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8825628 spots for SRR13712783/SRR13712783.sra Written 8825628 spots for SRR13712783/SRR13712783.sra Read 9305928 spots for SRR13712784/SRR13712784.sra Written 9305928 spots for SRR13712784/SRR13712784.sra Read 8854205 spots for SRR13712785/SRR13712785.sra Written 8854205 spots for SRR13712785/SRR13712785.sra Read 17496886 spots for SRR13712786/SRR13712786.sra Written 17496886 spots for SRR13712786/SRR13712786.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:44 44482647 reads; of these: 44482647 (100.00%) were unpaired; of these: 2030614 (4.56%) aligned 0 times 34539358 (77.65%) aligned exactly 1 time 7912675 (17.79%) aligned >1 times 95.44% overall alignment rate Time searching: 00:05:44 Overall time: 00:05:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 25515457 / 42452033 = 0.6010 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:38:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:38:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:38:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:38:59: 1000000 INFO @ Sat, 15 Jan 2022 18:39:04: 2000000 INFO @ Sat, 15 Jan 2022 18:39:10: 3000000 INFO @ Sat, 15 Jan 2022 18:39:15: 4000000 INFO @ Sat, 15 Jan 2022 18:39:21: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:39:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:39:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:39:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:39:26: 6000000 INFO @ Sat, 15 Jan 2022 18:39:29: 1000000 INFO @ Sat, 15 Jan 2022 18:39:32: 7000000 INFO @ Sat, 15 Jan 2022 18:39:35: 2000000 INFO @ Sat, 15 Jan 2022 18:39:37: 8000000 INFO @ Sat, 15 Jan 2022 18:39:40: 3000000 INFO @ Sat, 15 Jan 2022 18:39:43: 9000000 INFO @ Sat, 15 Jan 2022 18:39:46: 4000000 INFO @ Sat, 15 Jan 2022 18:39:48: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:39:52: 5000000 INFO @ Sat, 15 Jan 2022 18:39:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:39:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:39:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:39:54: 11000000 INFO @ Sat, 15 Jan 2022 18:39:58: 6000000 INFO @ Sat, 15 Jan 2022 18:39:58: 1000000 INFO @ Sat, 15 Jan 2022 18:39:59: 12000000 INFO @ Sat, 15 Jan 2022 18:40:03: 2000000 INFO @ Sat, 15 Jan 2022 18:40:03: 7000000 INFO @ Sat, 15 Jan 2022 18:40:05: 13000000 INFO @ Sat, 15 Jan 2022 18:40:08: 3000000 INFO @ Sat, 15 Jan 2022 18:40:09: 8000000 INFO @ Sat, 15 Jan 2022 18:40:10: 14000000 INFO @ Sat, 15 Jan 2022 18:40:13: 4000000 INFO @ Sat, 15 Jan 2022 18:40:14: 9000000 INFO @ Sat, 15 Jan 2022 18:40:16: 15000000 INFO @ Sat, 15 Jan 2022 18:40:18: 5000000 INFO @ Sat, 15 Jan 2022 18:40:20: 10000000 INFO @ Sat, 15 Jan 2022 18:40:21: 16000000 INFO @ Sat, 15 Jan 2022 18:40:23: 6000000 INFO @ Sat, 15 Jan 2022 18:40:25: 11000000 INFO @ Sat, 15 Jan 2022 18:40:26: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:40:26: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:40:26: #1 total tags in treatment: 16936576 INFO @ Sat, 15 Jan 2022 18:40:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:40:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:40:27: #1 tags after filtering in treatment: 16936576 INFO @ Sat, 15 Jan 2022 18:40:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:40:27: #1 finished! INFO @ Sat, 15 Jan 2022 18:40:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:40:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:40:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:40:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:40:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:40:28: 7000000 INFO @ Sat, 15 Jan 2022 18:40:31: 12000000 INFO @ Sat, 15 Jan 2022 18:40:33: 8000000 INFO @ Sat, 15 Jan 2022 18:40:36: 13000000 INFO @ Sat, 15 Jan 2022 18:40:38: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:40:42: 14000000 INFO @ Sat, 15 Jan 2022 18:40:43: 10000000 INFO @ Sat, 15 Jan 2022 18:40:47: 15000000 INFO @ Sat, 15 Jan 2022 18:40:48: 11000000 INFO @ Sat, 15 Jan 2022 18:40:53: 12000000 INFO @ Sat, 15 Jan 2022 18:40:53: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:40:58: 13000000 INFO @ Sat, 15 Jan 2022 18:40:58: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:40:58: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:40:58: #1 total tags in treatment: 16936576 INFO @ Sat, 15 Jan 2022 18:40:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:40:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:40:58: #1 tags after filtering in treatment: 16936576 INFO @ Sat, 15 Jan 2022 18:40:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:40:58: #1 finished! INFO @ Sat, 15 Jan 2022 18:40:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:40:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:40:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:40:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:40:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:41:02: 14000000 INFO @ Sat, 15 Jan 2022 18:41:07: 15000000 INFO @ Sat, 15 Jan 2022 18:41:12: 16000000 INFO @ Sat, 15 Jan 2022 18:41:16: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:41:16: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:41:16: #1 total tags in treatment: 16936576 INFO @ Sat, 15 Jan 2022 18:41:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:41:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:41:17: #1 tags after filtering in treatment: 16936576 INFO @ Sat, 15 Jan 2022 18:41:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:41:17: #1 finished! INFO @ Sat, 15 Jan 2022 18:41:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:41:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:41:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:41:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:41:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101474/SRX10101474.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling