Job ID = 14520089 SRX = SRX10101471 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7413939 spots for SRR13712771/SRR13712771.sra Written 7413939 spots for SRR13712771/SRR13712771.sra Read 10777498 spots for SRR13712772/SRR13712772.sra Written 10777498 spots for SRR13712772/SRR13712772.sra Read 10320133 spots for SRR13712773/SRR13712773.sra Written 10320133 spots for SRR13712773/SRR13712773.sra Read 11617479 spots for SRR13712774/SRR13712774.sra Written 11617479 spots for SRR13712774/SRR13712774.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:10 40129049 reads; of these: 40129049 (100.00%) were unpaired; of these: 1886413 (4.70%) aligned 0 times 31970065 (79.67%) aligned exactly 1 time 6272571 (15.63%) aligned >1 times 95.30% overall alignment rate Time searching: 00:06:10 Overall time: 00:06:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 22026140 / 38242636 = 0.5760 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:37:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:37:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:37:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:37:37: 1000000 INFO @ Sat, 15 Jan 2022 18:37:43: 2000000 INFO @ Sat, 15 Jan 2022 18:37:48: 3000000 INFO @ Sat, 15 Jan 2022 18:37:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:37:59: 5000000 INFO @ Sat, 15 Jan 2022 18:38:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:38:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:38:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:38:05: 6000000 INFO @ Sat, 15 Jan 2022 18:38:08: 1000000 INFO @ Sat, 15 Jan 2022 18:38:11: 7000000 INFO @ Sat, 15 Jan 2022 18:38:14: 2000000 INFO @ Sat, 15 Jan 2022 18:38:17: 8000000 INFO @ Sat, 15 Jan 2022 18:38:20: 3000000 INFO @ Sat, 15 Jan 2022 18:38:23: 9000000 INFO @ Sat, 15 Jan 2022 18:38:26: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:38:29: 10000000 INFO @ Sat, 15 Jan 2022 18:38:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:38:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:38:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:38:32: 5000000 INFO @ Sat, 15 Jan 2022 18:38:35: 11000000 INFO @ Sat, 15 Jan 2022 18:38:38: 1000000 INFO @ Sat, 15 Jan 2022 18:38:38: 6000000 INFO @ Sat, 15 Jan 2022 18:38:42: 12000000 INFO @ Sat, 15 Jan 2022 18:38:44: 2000000 INFO @ Sat, 15 Jan 2022 18:38:45: 7000000 INFO @ Sat, 15 Jan 2022 18:38:48: 13000000 INFO @ Sat, 15 Jan 2022 18:38:51: 3000000 INFO @ Sat, 15 Jan 2022 18:38:51: 8000000 INFO @ Sat, 15 Jan 2022 18:38:55: 14000000 INFO @ Sat, 15 Jan 2022 18:38:58: 4000000 INFO @ Sat, 15 Jan 2022 18:38:58: 9000000 INFO @ Sat, 15 Jan 2022 18:39:01: 15000000 INFO @ Sat, 15 Jan 2022 18:39:04: 5000000 INFO @ Sat, 15 Jan 2022 18:39:05: 10000000 INFO @ Sat, 15 Jan 2022 18:39:08: 16000000 INFO @ Sat, 15 Jan 2022 18:39:09: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:39:09: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:39:09: #1 total tags in treatment: 16216496 INFO @ Sat, 15 Jan 2022 18:39:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:39:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:39:09: #1 tags after filtering in treatment: 16216496 INFO @ Sat, 15 Jan 2022 18:39:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:39:09: #1 finished! INFO @ Sat, 15 Jan 2022 18:39:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:39:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:39:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:39:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:39:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Sat, 15 Jan 2022 18:39:10: 6000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:39:11: 11000000 INFO @ Sat, 15 Jan 2022 18:39:17: 7000000 INFO @ Sat, 15 Jan 2022 18:39:17: 12000000 INFO @ Sat, 15 Jan 2022 18:39:23: 8000000 INFO @ Sat, 15 Jan 2022 18:39:23: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:39:29: 9000000 INFO @ Sat, 15 Jan 2022 18:39:29: 14000000 INFO @ Sat, 15 Jan 2022 18:39:35: 10000000 INFO @ Sat, 15 Jan 2022 18:39:35: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:39:41: 11000000 INFO @ Sat, 15 Jan 2022 18:39:41: 16000000 INFO @ Sat, 15 Jan 2022 18:39:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:39:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:39:43: #1 total tags in treatment: 16216496 INFO @ Sat, 15 Jan 2022 18:39:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:39:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:39:43: #1 tags after filtering in treatment: 16216496 INFO @ Sat, 15 Jan 2022 18:39:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:39:43: #1 finished! INFO @ Sat, 15 Jan 2022 18:39:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:39:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:39:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:39:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:39:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:39:47: 12000000 INFO @ Sat, 15 Jan 2022 18:39:52: 13000000 INFO @ Sat, 15 Jan 2022 18:39:58: 14000000 INFO @ Sat, 15 Jan 2022 18:40:03: 15000000 INFO @ Sat, 15 Jan 2022 18:40:09: 16000000 INFO @ Sat, 15 Jan 2022 18:40:10: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:40:10: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:40:10: #1 total tags in treatment: 16216496 INFO @ Sat, 15 Jan 2022 18:40:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:40:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:40:10: #1 tags after filtering in treatment: 16216496 INFO @ Sat, 15 Jan 2022 18:40:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:40:10: #1 finished! INFO @ Sat, 15 Jan 2022 18:40:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:40:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:40:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:40:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:40:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101471/SRX10101471.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling