Job ID = 14520088 SRX = SRX10101470 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6225241 spots for SRR13712767/SRR13712767.sra Written 6225241 spots for SRR13712767/SRR13712767.sra Read 7774083 spots for SRR13712768/SRR13712768.sra Written 7774083 spots for SRR13712768/SRR13712768.sra Read 7604594 spots for SRR13712769/SRR13712769.sra Written 7604594 spots for SRR13712769/SRR13712769.sra Read 11791668 spots for SRR13712770/SRR13712770.sra Written 11791668 spots for SRR13712770/SRR13712770.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:02 33395586 reads; of these: 33395586 (100.00%) were unpaired; of these: 1880077 (5.63%) aligned 0 times 26112193 (78.19%) aligned exactly 1 time 5403316 (16.18%) aligned >1 times 94.37% overall alignment rate Time searching: 00:05:02 Overall time: 00:05:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 16783968 / 31515509 = 0.5326 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:34:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:34:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:34:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:34:52: 1000000 INFO @ Sat, 15 Jan 2022 18:34:57: 2000000 INFO @ Sat, 15 Jan 2022 18:35:02: 3000000 INFO @ Sat, 15 Jan 2022 18:35:07: 4000000 INFO @ Sat, 15 Jan 2022 18:35:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:35:16: 6000000 INFO @ Sat, 15 Jan 2022 18:35:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:35:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:35:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:35:21: 7000000 INFO @ Sat, 15 Jan 2022 18:35:22: 1000000 INFO @ Sat, 15 Jan 2022 18:35:27: 8000000 INFO @ Sat, 15 Jan 2022 18:35:27: 2000000 INFO @ Sat, 15 Jan 2022 18:35:32: 9000000 INFO @ Sat, 15 Jan 2022 18:35:33: 3000000 INFO @ Sat, 15 Jan 2022 18:35:37: 10000000 INFO @ Sat, 15 Jan 2022 18:35:38: 4000000 INFO @ Sat, 15 Jan 2022 18:35:42: 11000000 INFO @ Sat, 15 Jan 2022 18:35:43: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:35:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:35:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:35:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:35:48: 12000000 INFO @ Sat, 15 Jan 2022 18:35:49: 6000000 INFO @ Sat, 15 Jan 2022 18:35:53: 1000000 INFO @ Sat, 15 Jan 2022 18:35:53: 13000000 INFO @ Sat, 15 Jan 2022 18:35:54: 7000000 INFO @ Sat, 15 Jan 2022 18:35:59: 14000000 INFO @ Sat, 15 Jan 2022 18:35:59: 2000000 INFO @ Sat, 15 Jan 2022 18:36:00: 8000000 INFO @ Sat, 15 Jan 2022 18:36:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:36:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:36:03: #1 total tags in treatment: 14731541 INFO @ Sat, 15 Jan 2022 18:36:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:36:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:36:03: #1 tags after filtering in treatment: 14731541 INFO @ Sat, 15 Jan 2022 18:36:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:36:03: #1 finished! INFO @ Sat, 15 Jan 2022 18:36:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:36:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:36:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:36:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:36:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:36:06: 9000000 INFO @ Sat, 15 Jan 2022 18:36:06: 3000000 INFO @ Sat, 15 Jan 2022 18:36:11: 10000000 INFO @ Sat, 15 Jan 2022 18:36:12: 4000000 INFO @ Sat, 15 Jan 2022 18:36:17: 11000000 INFO @ Sat, 15 Jan 2022 18:36:18: 5000000 INFO @ Sat, 15 Jan 2022 18:36:22: 12000000 INFO @ Sat, 15 Jan 2022 18:36:24: 6000000 INFO @ Sat, 15 Jan 2022 18:36:28: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:36:30: 7000000 INFO @ Sat, 15 Jan 2022 18:36:33: 14000000 INFO @ Sat, 15 Jan 2022 18:36:36: 8000000 INFO @ Sat, 15 Jan 2022 18:36:38: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:36:38: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:36:38: #1 total tags in treatment: 14731541 INFO @ Sat, 15 Jan 2022 18:36:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:36:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:36:38: #1 tags after filtering in treatment: 14731541 INFO @ Sat, 15 Jan 2022 18:36:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:36:38: #1 finished! INFO @ Sat, 15 Jan 2022 18:36:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:36:38: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:36:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:36:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:36:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:36:43: 9000000 INFO @ Sat, 15 Jan 2022 18:36:49: 10000000 INFO @ Sat, 15 Jan 2022 18:36:54: 11000000 INFO @ Sat, 15 Jan 2022 18:37:00: 12000000 INFO @ Sat, 15 Jan 2022 18:37:06: 13000000 INFO @ Sat, 15 Jan 2022 18:37:12: 14000000 INFO @ Sat, 15 Jan 2022 18:37:16: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:37:16: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:37:16: #1 total tags in treatment: 14731541 INFO @ Sat, 15 Jan 2022 18:37:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:37:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:37:16: #1 tags after filtering in treatment: 14731541 INFO @ Sat, 15 Jan 2022 18:37:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:37:16: #1 finished! INFO @ Sat, 15 Jan 2022 18:37:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:37:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:37:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:37:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:37:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101470/SRX10101470.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling