Job ID = 14520000 SRX = SRX10101469 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8736279 spots for SRR13712763/SRR13712763.sra Written 8736279 spots for SRR13712763/SRR13712763.sra Read 10968118 spots for SRR13712764/SRR13712764.sra Written 10968118 spots for SRR13712764/SRR13712764.sra Read 14047089 spots for SRR13712765/SRR13712765.sra Written 14047089 spots for SRR13712765/SRR13712765.sra Read 5088616 spots for SRR13712766/SRR13712766.sra Written 5088616 spots for SRR13712766/SRR13712766.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:12 38840102 reads; of these: 38840102 (100.00%) were unpaired; of these: 1325294 (3.41%) aligned 0 times 29641633 (76.32%) aligned exactly 1 time 7873175 (20.27%) aligned >1 times 96.59% overall alignment rate Time searching: 00:06:12 Overall time: 00:06:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 20838512 / 37514808 = 0.5555 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:31:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:31:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:31:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:31:09: 1000000 INFO @ Sat, 15 Jan 2022 18:31:14: 2000000 INFO @ Sat, 15 Jan 2022 18:31:19: 3000000 INFO @ Sat, 15 Jan 2022 18:31:24: 4000000 INFO @ Sat, 15 Jan 2022 18:31:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:31:33: 6000000 INFO @ Sat, 15 Jan 2022 18:31:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:31:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:31:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:31:39: 7000000 INFO @ Sat, 15 Jan 2022 18:31:39: 1000000 INFO @ Sat, 15 Jan 2022 18:31:44: 8000000 INFO @ Sat, 15 Jan 2022 18:31:45: 2000000 INFO @ Sat, 15 Jan 2022 18:31:51: 9000000 INFO @ Sat, 15 Jan 2022 18:31:51: 3000000 INFO @ Sat, 15 Jan 2022 18:31:57: 10000000 INFO @ Sat, 15 Jan 2022 18:31:58: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:32:03: 11000000 INFO @ Sat, 15 Jan 2022 18:32:04: 5000000 INFO @ Sat, 15 Jan 2022 18:32:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:32:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:32:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:32:09: 1000000 INFO @ Sat, 15 Jan 2022 18:32:10: 12000000 INFO @ Sat, 15 Jan 2022 18:32:10: 6000000 INFO @ Sat, 15 Jan 2022 18:32:14: 2000000 INFO @ Sat, 15 Jan 2022 18:32:16: 13000000 INFO @ Sat, 15 Jan 2022 18:32:16: 7000000 INFO @ Sat, 15 Jan 2022 18:32:19: 3000000 INFO @ Sat, 15 Jan 2022 18:32:22: 14000000 INFO @ Sat, 15 Jan 2022 18:32:22: 8000000 INFO @ Sat, 15 Jan 2022 18:32:24: 4000000 INFO @ Sat, 15 Jan 2022 18:32:28: 15000000 INFO @ Sat, 15 Jan 2022 18:32:29: 9000000 INFO @ Sat, 15 Jan 2022 18:32:30: 5000000 INFO @ Sat, 15 Jan 2022 18:32:34: 16000000 INFO @ Sat, 15 Jan 2022 18:32:34: 10000000 INFO @ Sat, 15 Jan 2022 18:32:36: 6000000 INFO @ Sat, 15 Jan 2022 18:32:38: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:32:38: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:32:38: #1 total tags in treatment: 16676296 INFO @ Sat, 15 Jan 2022 18:32:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:32:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:32:38: #1 tags after filtering in treatment: 16676296 INFO @ Sat, 15 Jan 2022 18:32:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:32:38: #1 finished! INFO @ Sat, 15 Jan 2022 18:32:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:32:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:32:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:32:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:32:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:32:40: 11000000 INFO @ Sat, 15 Jan 2022 18:32:41: 7000000 INFO @ Sat, 15 Jan 2022 18:32:45: 12000000 INFO @ Sat, 15 Jan 2022 18:32:47: 8000000 INFO @ Sat, 15 Jan 2022 18:32:51: 13000000 INFO @ Sat, 15 Jan 2022 18:32:52: 9000000 INFO @ Sat, 15 Jan 2022 18:32:57: 14000000 INFO @ Sat, 15 Jan 2022 18:32:57: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:33:02: 11000000 INFO @ Sat, 15 Jan 2022 18:33:03: 15000000 INFO @ Sat, 15 Jan 2022 18:33:07: 12000000 INFO @ Sat, 15 Jan 2022 18:33:10: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:33:12: 13000000 INFO @ Sat, 15 Jan 2022 18:33:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:33:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:33:14: #1 total tags in treatment: 16676296 INFO @ Sat, 15 Jan 2022 18:33:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:33:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:33:14: #1 tags after filtering in treatment: 16676296 INFO @ Sat, 15 Jan 2022 18:33:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:33:14: #1 finished! INFO @ Sat, 15 Jan 2022 18:33:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:33:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:33:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:33:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:33:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:33:17: 14000000 INFO @ Sat, 15 Jan 2022 18:33:22: 15000000 INFO @ Sat, 15 Jan 2022 18:33:28: 16000000 INFO @ Sat, 15 Jan 2022 18:33:32: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:33:32: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:33:32: #1 total tags in treatment: 16676296 INFO @ Sat, 15 Jan 2022 18:33:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:33:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:33:32: #1 tags after filtering in treatment: 16676296 INFO @ Sat, 15 Jan 2022 18:33:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:33:32: #1 finished! INFO @ Sat, 15 Jan 2022 18:33:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:33:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:33:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:33:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:33:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101469/SRX10101469.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling