Job ID = 14519981 SRX = SRX10101452 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10105299 spots for SRR13712759/SRR13712759.sra Written 10105299 spots for SRR13712759/SRR13712759.sra Read 10196684 spots for SRR13712760/SRR13712760.sra Written 10196684 spots for SRR13712760/SRR13712760.sra Read 14550471 spots for SRR13712761/SRR13712761.sra Written 14550471 spots for SRR13712761/SRR13712761.sra Read 4082779 spots for SRR13712762/SRR13712762.sra Written 4082779 spots for SRR13712762/SRR13712762.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:20 38935233 reads; of these: 38935233 (100.00%) were unpaired; of these: 1327165 (3.41%) aligned 0 times 29884181 (76.75%) aligned exactly 1 time 7723887 (19.84%) aligned >1 times 96.59% overall alignment rate Time searching: 00:08:20 Overall time: 00:08:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 20849049 / 37608068 = 0.5544 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:27:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:27:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:27:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:27:50: 1000000 INFO @ Sat, 15 Jan 2022 18:27:56: 2000000 INFO @ Sat, 15 Jan 2022 18:28:01: 3000000 INFO @ Sat, 15 Jan 2022 18:28:07: 4000000 INFO @ Sat, 15 Jan 2022 18:28:12: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:28:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:28:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:28:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:28:18: 6000000 INFO @ Sat, 15 Jan 2022 18:28:21: 1000000 INFO @ Sat, 15 Jan 2022 18:28:24: 7000000 INFO @ Sat, 15 Jan 2022 18:28:28: 2000000 INFO @ Sat, 15 Jan 2022 18:28:31: 8000000 INFO @ Sat, 15 Jan 2022 18:28:36: 3000000 INFO @ Sat, 15 Jan 2022 18:28:37: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:28:43: 4000000 INFO @ Sat, 15 Jan 2022 18:28:44: 10000000 INFO @ Sat, 15 Jan 2022 18:28:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:28:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:28:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:28:50: 5000000 INFO @ Sat, 15 Jan 2022 18:28:50: 11000000 INFO @ Sat, 15 Jan 2022 18:28:51: 1000000 INFO @ Sat, 15 Jan 2022 18:28:57: 12000000 INFO @ Sat, 15 Jan 2022 18:28:57: 2000000 INFO @ Sat, 15 Jan 2022 18:28:57: 6000000 INFO @ Sat, 15 Jan 2022 18:29:03: 13000000 INFO @ Sat, 15 Jan 2022 18:29:04: 3000000 INFO @ Sat, 15 Jan 2022 18:29:05: 7000000 INFO @ Sat, 15 Jan 2022 18:29:10: 14000000 INFO @ Sat, 15 Jan 2022 18:29:11: 4000000 INFO @ Sat, 15 Jan 2022 18:29:12: 8000000 INFO @ Sat, 15 Jan 2022 18:29:16: 15000000 INFO @ Sat, 15 Jan 2022 18:29:17: 5000000 INFO @ Sat, 15 Jan 2022 18:29:19: 9000000 INFO @ Sat, 15 Jan 2022 18:29:23: 16000000 INFO @ Sat, 15 Jan 2022 18:29:24: 6000000 INFO @ Sat, 15 Jan 2022 18:29:27: 10000000 INFO @ Sat, 15 Jan 2022 18:29:28: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:29:28: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:29:28: #1 total tags in treatment: 16759019 INFO @ Sat, 15 Jan 2022 18:29:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:29:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:29:28: #1 tags after filtering in treatment: 16759019 INFO @ Sat, 15 Jan 2022 18:29:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:29:28: #1 finished! INFO @ Sat, 15 Jan 2022 18:29:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:29:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:29:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:29:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:29:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:29:31: 7000000 INFO @ Sat, 15 Jan 2022 18:29:34: 11000000 INFO @ Sat, 15 Jan 2022 18:29:37: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:29:41: 12000000 INFO @ Sat, 15 Jan 2022 18:29:44: 9000000 INFO @ Sat, 15 Jan 2022 18:29:48: 13000000 INFO @ Sat, 15 Jan 2022 18:29:50: 10000000 INFO @ Sat, 15 Jan 2022 18:29:55: 14000000 INFO @ Sat, 15 Jan 2022 18:29:57: 11000000 INFO @ Sat, 15 Jan 2022 18:30:03: 15000000 INFO @ Sat, 15 Jan 2022 18:30:04: 12000000 INFO @ Sat, 15 Jan 2022 18:30:10: 16000000 INFO @ Sat, 15 Jan 2022 18:30:10: 13000000 INFO @ Sat, 15 Jan 2022 18:30:15: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:30:15: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:30:15: #1 total tags in treatment: 16759019 INFO @ Sat, 15 Jan 2022 18:30:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:30:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:30:15: #1 tags after filtering in treatment: 16759019 INFO @ Sat, 15 Jan 2022 18:30:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:30:15: #1 finished! INFO @ Sat, 15 Jan 2022 18:30:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:30:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:30:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:30:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:30:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:30:16: 14000000 INFO @ Sat, 15 Jan 2022 18:30:22: 15000000 INFO @ Sat, 15 Jan 2022 18:30:27: 16000000 INFO @ Sat, 15 Jan 2022 18:30:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:30:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:30:31: #1 total tags in treatment: 16759019 INFO @ Sat, 15 Jan 2022 18:30:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:30:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:30:32: #1 tags after filtering in treatment: 16759019 INFO @ Sat, 15 Jan 2022 18:30:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:30:32: #1 finished! INFO @ Sat, 15 Jan 2022 18:30:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:30:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:30:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:30:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:30:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101452/SRX10101452.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling