Job ID = 14519977 SRX = SRX10101451 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8480148 spots for SRR13712755/SRR13712755.sra Written 8480148 spots for SRR13712755/SRR13712755.sra Read 9036132 spots for SRR13712756/SRR13712756.sra Written 9036132 spots for SRR13712756/SRR13712756.sra Read 13007722 spots for SRR13712757/SRR13712757.sra Written 13007722 spots for SRR13712757/SRR13712757.sra Read 5011658 spots for SRR13712758/SRR13712758.sra Written 5011658 spots for SRR13712758/SRR13712758.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:01 35535660 reads; of these: 35535660 (100.00%) were unpaired; of these: 1522981 (4.29%) aligned 0 times 27041977 (76.10%) aligned exactly 1 time 6970702 (19.62%) aligned >1 times 95.71% overall alignment rate Time searching: 00:05:01 Overall time: 00:05:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18168244 / 34012679 = 0.5342 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:23:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:23:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:23:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:23:38: 1000000 INFO @ Sat, 15 Jan 2022 18:23:44: 2000000 INFO @ Sat, 15 Jan 2022 18:23:49: 3000000 INFO @ Sat, 15 Jan 2022 18:23:54: 4000000 INFO @ Sat, 15 Jan 2022 18:24:00: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:24:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:24:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:24:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:24:06: 6000000 INFO @ Sat, 15 Jan 2022 18:24:09: 1000000 INFO @ Sat, 15 Jan 2022 18:24:12: 7000000 INFO @ Sat, 15 Jan 2022 18:24:16: 2000000 INFO @ Sat, 15 Jan 2022 18:24:19: 8000000 INFO @ Sat, 15 Jan 2022 18:24:24: 3000000 INFO @ Sat, 15 Jan 2022 18:24:25: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:24:31: 4000000 INFO @ Sat, 15 Jan 2022 18:24:32: 10000000 INFO @ Sat, 15 Jan 2022 18:24:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:24:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:24:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:24:38: 5000000 INFO @ Sat, 15 Jan 2022 18:24:39: 11000000 INFO @ Sat, 15 Jan 2022 18:24:40: 1000000 INFO @ Sat, 15 Jan 2022 18:24:45: 6000000 INFO @ Sat, 15 Jan 2022 18:24:45: 12000000 INFO @ Sat, 15 Jan 2022 18:24:47: 2000000 INFO @ Sat, 15 Jan 2022 18:24:52: 13000000 INFO @ Sat, 15 Jan 2022 18:24:53: 7000000 INFO @ Sat, 15 Jan 2022 18:24:55: 3000000 INFO @ Sat, 15 Jan 2022 18:24:59: 14000000 INFO @ Sat, 15 Jan 2022 18:25:00: 8000000 INFO @ Sat, 15 Jan 2022 18:25:02: 4000000 INFO @ Sat, 15 Jan 2022 18:25:06: 15000000 INFO @ Sat, 15 Jan 2022 18:25:07: 9000000 INFO @ Sat, 15 Jan 2022 18:25:10: 5000000 INFO @ Sat, 15 Jan 2022 18:25:12: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:25:12: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:25:12: #1 total tags in treatment: 15844435 INFO @ Sat, 15 Jan 2022 18:25:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:25:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:25:12: #1 tags after filtering in treatment: 15844435 INFO @ Sat, 15 Jan 2022 18:25:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:25:12: #1 finished! INFO @ Sat, 15 Jan 2022 18:25:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:25:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:25:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:25:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:25:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:25:15: 10000000 INFO @ Sat, 15 Jan 2022 18:25:17: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:25:22: 11000000 INFO @ Sat, 15 Jan 2022 18:25:25: 7000000 INFO @ Sat, 15 Jan 2022 18:25:29: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:25:32: 8000000 INFO @ Sat, 15 Jan 2022 18:25:37: 13000000 INFO @ Sat, 15 Jan 2022 18:25:40: 9000000 INFO @ Sat, 15 Jan 2022 18:25:44: 14000000 INFO @ Sat, 15 Jan 2022 18:25:47: 10000000 INFO @ Sat, 15 Jan 2022 18:25:51: 15000000 INFO @ Sat, 15 Jan 2022 18:25:55: 11000000 INFO @ Sat, 15 Jan 2022 18:25:57: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:25:57: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:25:57: #1 total tags in treatment: 15844435 INFO @ Sat, 15 Jan 2022 18:25:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:25:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:25:57: #1 tags after filtering in treatment: 15844435 INFO @ Sat, 15 Jan 2022 18:25:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:25:57: #1 finished! INFO @ Sat, 15 Jan 2022 18:25:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:25:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:25:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:25:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:25:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:26:02: 12000000 INFO @ Sat, 15 Jan 2022 18:26:09: 13000000 INFO @ Sat, 15 Jan 2022 18:26:16: 14000000 INFO @ Sat, 15 Jan 2022 18:26:22: 15000000 INFO @ Sat, 15 Jan 2022 18:26:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:26:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:26:27: #1 total tags in treatment: 15844435 INFO @ Sat, 15 Jan 2022 18:26:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:26:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:26:28: #1 tags after filtering in treatment: 15844435 INFO @ Sat, 15 Jan 2022 18:26:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:26:28: #1 finished! INFO @ Sat, 15 Jan 2022 18:26:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:26:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:26:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:26:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:26:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101451/SRX10101451.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling