Job ID = 14519969 SRX = SRX10101450 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 9813719 spots for SRR13712751/SRR13712751.sra Written 9813719 spots for SRR13712751/SRR13712751.sra Read 9774579 spots for SRR13712752/SRR13712752.sra Written 9774579 spots for SRR13712752/SRR13712752.sra Read 13379802 spots for SRR13712753/SRR13712753.sra Written 13379802 spots for SRR13712753/SRR13712753.sra Read 11178440 spots for SRR13712754/SRR13712754.sra Written 11178440 spots for SRR13712754/SRR13712754.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:00 44146540 reads; of these: 44146540 (100.00%) were unpaired; of these: 1496598 (3.39%) aligned 0 times 34094969 (77.23%) aligned exactly 1 time 8554973 (19.38%) aligned >1 times 96.61% overall alignment rate Time searching: 00:06:00 Overall time: 00:06:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 25019399 / 42649942 = 0.5866 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:26:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:26:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:26:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:26:32: 1000000 INFO @ Sat, 15 Jan 2022 18:26:36: 2000000 INFO @ Sat, 15 Jan 2022 18:26:41: 3000000 INFO @ Sat, 15 Jan 2022 18:26:45: 4000000 INFO @ Sat, 15 Jan 2022 18:26:50: 5000000 INFO @ Sat, 15 Jan 2022 18:26:54: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:26:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:26:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:26:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:26:59: 7000000 INFO @ Sat, 15 Jan 2022 18:27:02: 1000000 INFO @ Sat, 15 Jan 2022 18:27:03: 8000000 INFO @ Sat, 15 Jan 2022 18:27:06: 2000000 INFO @ Sat, 15 Jan 2022 18:27:08: 9000000 INFO @ Sat, 15 Jan 2022 18:27:11: 3000000 INFO @ Sat, 15 Jan 2022 18:27:13: 10000000 INFO @ Sat, 15 Jan 2022 18:27:16: 4000000 INFO @ Sat, 15 Jan 2022 18:27:17: 11000000 INFO @ Sat, 15 Jan 2022 18:27:20: 5000000 INFO @ Sat, 15 Jan 2022 18:27:22: 12000000 BedGraph に変換中... INFO @ Sat, 15 Jan 2022 18:27:25: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:27:27: 13000000 INFO @ Sat, 15 Jan 2022 18:27:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:27:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:27:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:27:30: 7000000 INFO @ Sat, 15 Jan 2022 18:27:31: 14000000 INFO @ Sat, 15 Jan 2022 18:27:32: 1000000 INFO @ Sat, 15 Jan 2022 18:27:35: 8000000 INFO @ Sat, 15 Jan 2022 18:27:36: 15000000 INFO @ Sat, 15 Jan 2022 18:27:38: 2000000 INFO @ Sat, 15 Jan 2022 18:27:39: 9000000 INFO @ Sat, 15 Jan 2022 18:27:41: 16000000 INFO @ Sat, 15 Jan 2022 18:27:43: 3000000 INFO @ Sat, 15 Jan 2022 18:27:44: 10000000 INFO @ Sat, 15 Jan 2022 18:27:46: 17000000 INFO @ Sat, 15 Jan 2022 18:27:49: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:27:49: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:27:49: #1 total tags in treatment: 17630543 INFO @ Sat, 15 Jan 2022 18:27:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:27:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:27:49: 11000000 INFO @ Sat, 15 Jan 2022 18:27:49: 4000000 INFO @ Sat, 15 Jan 2022 18:27:49: #1 tags after filtering in treatment: 17630543 INFO @ Sat, 15 Jan 2022 18:27:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:27:49: #1 finished! INFO @ Sat, 15 Jan 2022 18:27:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:27:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:27:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:27:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:27:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:27:54: 12000000 INFO @ Sat, 15 Jan 2022 18:27:54: 5000000 INFO @ Sat, 15 Jan 2022 18:27:58: 13000000 INFO @ Sat, 15 Jan 2022 18:28:00: 6000000 INFO @ Sat, 15 Jan 2022 18:28:03: 14000000 INFO @ Sat, 15 Jan 2022 18:28:05: 7000000 INFO @ Sat, 15 Jan 2022 18:28:08: 15000000 INFO @ Sat, 15 Jan 2022 18:28:11: 8000000 INFO @ Sat, 15 Jan 2022 18:28:13: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:28:16: 9000000 INFO @ Sat, 15 Jan 2022 18:28:17: 17000000 INFO @ Sat, 15 Jan 2022 18:28:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:28:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:28:20: #1 total tags in treatment: 17630543 INFO @ Sat, 15 Jan 2022 18:28:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:28:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:28:21: #1 tags after filtering in treatment: 17630543 INFO @ Sat, 15 Jan 2022 18:28:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:28:21: #1 finished! INFO @ Sat, 15 Jan 2022 18:28:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:28:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:28:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:28:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:28:22: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 18:28:22: 10000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:28:27: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:28:32: 12000000 INFO @ Sat, 15 Jan 2022 18:28:38: 13000000 INFO @ Sat, 15 Jan 2022 18:28:43: 14000000 INFO @ Sat, 15 Jan 2022 18:28:49: 15000000 INFO @ Sat, 15 Jan 2022 18:28:54: 16000000 INFO @ Sat, 15 Jan 2022 18:28:59: 17000000 INFO @ Sat, 15 Jan 2022 18:29:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:29:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:29:03: #1 total tags in treatment: 17630543 INFO @ Sat, 15 Jan 2022 18:29:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:29:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:29:03: #1 tags after filtering in treatment: 17630543 INFO @ Sat, 15 Jan 2022 18:29:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:29:03: #1 finished! INFO @ Sat, 15 Jan 2022 18:29:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:29:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:29:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:29:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:29:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101450/SRX10101450.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling