Job ID = 14519936 SRX = SRX10101449 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8579471 spots for SRR13712747/SRR13712747.sra Written 8579471 spots for SRR13712747/SRR13712747.sra Read 10119973 spots for SRR13712748/SRR13712748.sra Written 10119973 spots for SRR13712748/SRR13712748.sra Read 12581414 spots for SRR13712749/SRR13712749.sra Written 12581414 spots for SRR13712749/SRR13712749.sra Read 16549180 spots for SRR13712750/SRR13712750.sra Written 16549180 spots for SRR13712750/SRR13712750.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:09 47830038 reads; of these: 47830038 (100.00%) were unpaired; of these: 1691723 (3.54%) aligned 0 times 37200246 (77.78%) aligned exactly 1 time 8938069 (18.69%) aligned >1 times 96.46% overall alignment rate Time searching: 00:06:09 Overall time: 00:06:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 27725163 / 46138315 = 0.6009 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:21:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:21:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:21:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:22:00: 1000000 INFO @ Sat, 15 Jan 2022 18:22:05: 2000000 INFO @ Sat, 15 Jan 2022 18:22:11: 3000000 INFO @ Sat, 15 Jan 2022 18:22:16: 4000000 INFO @ Sat, 15 Jan 2022 18:22:22: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:22:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:22:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:22:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:22:27: 6000000 INFO @ Sat, 15 Jan 2022 18:22:30: 1000000 INFO @ Sat, 15 Jan 2022 18:22:33: 7000000 INFO @ Sat, 15 Jan 2022 18:22:36: 2000000 INFO @ Sat, 15 Jan 2022 18:22:39: 8000000 INFO @ Sat, 15 Jan 2022 18:22:42: 3000000 INFO @ Sat, 15 Jan 2022 18:22:45: 9000000 INFO @ Sat, 15 Jan 2022 18:22:49: 4000000 INFO @ Sat, 15 Jan 2022 18:22:52: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:22:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:22:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:22:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:22:55: 5000000 INFO @ Sat, 15 Jan 2022 18:22:58: 11000000 INFO @ Sat, 15 Jan 2022 18:23:01: 1000000 INFO @ Sat, 15 Jan 2022 18:23:02: 6000000 INFO @ Sat, 15 Jan 2022 18:23:05: 12000000 INFO @ Sat, 15 Jan 2022 18:23:08: 7000000 INFO @ Sat, 15 Jan 2022 18:23:09: 2000000 INFO @ Sat, 15 Jan 2022 18:23:11: 13000000 INFO @ Sat, 15 Jan 2022 18:23:15: 8000000 INFO @ Sat, 15 Jan 2022 18:23:16: 3000000 INFO @ Sat, 15 Jan 2022 18:23:18: 14000000 INFO @ Sat, 15 Jan 2022 18:23:22: 9000000 INFO @ Sat, 15 Jan 2022 18:23:23: 4000000 INFO @ Sat, 15 Jan 2022 18:23:24: 15000000 INFO @ Sat, 15 Jan 2022 18:23:28: 10000000 INFO @ Sat, 15 Jan 2022 18:23:30: 5000000 INFO @ Sat, 15 Jan 2022 18:23:31: 16000000 INFO @ Sat, 15 Jan 2022 18:23:35: 11000000 INFO @ Sat, 15 Jan 2022 18:23:37: 6000000 INFO @ Sat, 15 Jan 2022 18:23:38: 17000000 INFO @ Sat, 15 Jan 2022 18:23:41: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:23:44: 7000000 INFO @ Sat, 15 Jan 2022 18:23:44: 18000000 INFO @ Sat, 15 Jan 2022 18:23:47: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:23:47: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:23:47: #1 total tags in treatment: 18413152 INFO @ Sat, 15 Jan 2022 18:23:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:23:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:23:47: #1 tags after filtering in treatment: 18413152 INFO @ Sat, 15 Jan 2022 18:23:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:23:47: #1 finished! INFO @ Sat, 15 Jan 2022 18:23:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:23:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:23:48: 13000000 INFO @ Sat, 15 Jan 2022 18:23:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:23:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:23:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:23:51: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:23:55: 14000000 INFO @ Sat, 15 Jan 2022 18:23:58: 9000000 INFO @ Sat, 15 Jan 2022 18:24:01: 15000000 INFO @ Sat, 15 Jan 2022 18:24:06: 10000000 INFO @ Sat, 15 Jan 2022 18:24:08: 16000000 INFO @ Sat, 15 Jan 2022 18:24:13: 11000000 INFO @ Sat, 15 Jan 2022 18:24:15: 17000000 INFO @ Sat, 15 Jan 2022 18:24:20: 12000000 INFO @ Sat, 15 Jan 2022 18:24:21: 18000000 INFO @ Sat, 15 Jan 2022 18:24:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:24:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:24:24: #1 total tags in treatment: 18413152 INFO @ Sat, 15 Jan 2022 18:24:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:24:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:24:24: #1 tags after filtering in treatment: 18413152 INFO @ Sat, 15 Jan 2022 18:24:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:24:24: #1 finished! INFO @ Sat, 15 Jan 2022 18:24:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:24:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:24:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:24:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:24:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:24:27: 13000000 INFO @ Sat, 15 Jan 2022 18:24:33: 14000000 INFO @ Sat, 15 Jan 2022 18:24:39: 15000000 INFO @ Sat, 15 Jan 2022 18:24:46: 16000000 INFO @ Sat, 15 Jan 2022 18:24:52: 17000000 INFO @ Sat, 15 Jan 2022 18:24:59: 18000000 INFO @ Sat, 15 Jan 2022 18:25:01: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:25:01: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:25:01: #1 total tags in treatment: 18413152 INFO @ Sat, 15 Jan 2022 18:25:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:25:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:25:01: #1 tags after filtering in treatment: 18413152 INFO @ Sat, 15 Jan 2022 18:25:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:25:01: #1 finished! INFO @ Sat, 15 Jan 2022 18:25:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:25:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:25:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:25:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:25:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101449/SRX10101449.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling