Job ID = 14519934 SRX = SRX10101448 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10076054 spots for SRR13712743/SRR13712743.sra Written 10076054 spots for SRR13712743/SRR13712743.sra Read 7194294 spots for SRR13712744/SRR13712744.sra Written 7194294 spots for SRR13712744/SRR13712744.sra Read 14381255 spots for SRR13712745/SRR13712745.sra Written 14381255 spots for SRR13712745/SRR13712745.sra Read 20386470 spots for SRR13712746/SRR13712746.sra Written 20386470 spots for SRR13712746/SRR13712746.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:32 52038073 reads; of these: 52038073 (100.00%) were unpaired; of these: 1823966 (3.51%) aligned 0 times 41096282 (78.97%) aligned exactly 1 time 9117825 (17.52%) aligned >1 times 96.49% overall alignment rate Time searching: 00:07:32 Overall time: 00:07:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 30978542 / 50214107 = 0.6169 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:23:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:23:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:23:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:23:43: 1000000 INFO @ Sat, 15 Jan 2022 18:23:48: 2000000 INFO @ Sat, 15 Jan 2022 18:23:53: 3000000 INFO @ Sat, 15 Jan 2022 18:23:57: 4000000 INFO @ Sat, 15 Jan 2022 18:24:02: 5000000 INFO @ Sat, 15 Jan 2022 18:24:07: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:24:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:24:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:24:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:24:11: 7000000 INFO @ Sat, 15 Jan 2022 18:24:14: 1000000 INFO @ Sat, 15 Jan 2022 18:24:16: 8000000 INFO @ Sat, 15 Jan 2022 18:24:19: 2000000 INFO @ Sat, 15 Jan 2022 18:24:21: 9000000 INFO @ Sat, 15 Jan 2022 18:24:24: 3000000 INFO @ Sat, 15 Jan 2022 18:24:26: 10000000 INFO @ Sat, 15 Jan 2022 18:24:29: 4000000 INFO @ Sat, 15 Jan 2022 18:24:31: 11000000 INFO @ Sat, 15 Jan 2022 18:24:33: 5000000 INFO @ Sat, 15 Jan 2022 18:24:36: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:24:38: 6000000 INFO @ Sat, 15 Jan 2022 18:24:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:24:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:24:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:24:41: 13000000 INFO @ Sat, 15 Jan 2022 18:24:43: 7000000 INFO @ Sat, 15 Jan 2022 18:24:44: 1000000 INFO @ Sat, 15 Jan 2022 18:24:46: 14000000 INFO @ Sat, 15 Jan 2022 18:24:48: 8000000 INFO @ Sat, 15 Jan 2022 18:24:49: 2000000 INFO @ Sat, 15 Jan 2022 18:24:51: 15000000 INFO @ Sat, 15 Jan 2022 18:24:53: 9000000 INFO @ Sat, 15 Jan 2022 18:24:54: 3000000 INFO @ Sat, 15 Jan 2022 18:24:56: 16000000 INFO @ Sat, 15 Jan 2022 18:24:58: 10000000 INFO @ Sat, 15 Jan 2022 18:24:59: 4000000 INFO @ Sat, 15 Jan 2022 18:25:01: 17000000 INFO @ Sat, 15 Jan 2022 18:25:03: 11000000 INFO @ Sat, 15 Jan 2022 18:25:04: 5000000 INFO @ Sat, 15 Jan 2022 18:25:06: 18000000 INFO @ Sat, 15 Jan 2022 18:25:08: 12000000 INFO @ Sat, 15 Jan 2022 18:25:08: 6000000 INFO @ Sat, 15 Jan 2022 18:25:11: 19000000 INFO @ Sat, 15 Jan 2022 18:25:12: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:25:12: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:25:12: #1 total tags in treatment: 19235565 INFO @ Sat, 15 Jan 2022 18:25:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:25:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:25:12: #1 tags after filtering in treatment: 19235565 INFO @ Sat, 15 Jan 2022 18:25:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:25:12: #1 finished! INFO @ Sat, 15 Jan 2022 18:25:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:25:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:25:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:25:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:25:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 18:25:13: 13000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:25:14: 7000000 INFO @ Sat, 15 Jan 2022 18:25:18: 14000000 INFO @ Sat, 15 Jan 2022 18:25:19: 8000000 INFO @ Sat, 15 Jan 2022 18:25:23: 15000000 INFO @ Sat, 15 Jan 2022 18:25:24: 9000000 INFO @ Sat, 15 Jan 2022 18:25:28: 16000000 INFO @ Sat, 15 Jan 2022 18:25:29: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:25:33: 17000000 INFO @ Sat, 15 Jan 2022 18:25:33: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:25:38: 18000000 INFO @ Sat, 15 Jan 2022 18:25:38: 12000000 INFO @ Sat, 15 Jan 2022 18:25:43: 19000000 INFO @ Sat, 15 Jan 2022 18:25:43: 13000000 INFO @ Sat, 15 Jan 2022 18:25:44: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:25:44: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:25:44: #1 total tags in treatment: 19235565 INFO @ Sat, 15 Jan 2022 18:25:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:25:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:25:44: #1 tags after filtering in treatment: 19235565 INFO @ Sat, 15 Jan 2022 18:25:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:25:44: #1 finished! INFO @ Sat, 15 Jan 2022 18:25:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:25:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:25:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:25:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:25:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:25:48: 14000000 INFO @ Sat, 15 Jan 2022 18:25:54: 15000000 INFO @ Sat, 15 Jan 2022 18:25:58: 16000000 INFO @ Sat, 15 Jan 2022 18:26:03: 17000000 INFO @ Sat, 15 Jan 2022 18:26:07: 18000000 INFO @ Sat, 15 Jan 2022 18:26:12: 19000000 INFO @ Sat, 15 Jan 2022 18:26:13: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:26:13: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:26:13: #1 total tags in treatment: 19235565 INFO @ Sat, 15 Jan 2022 18:26:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:26:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:26:14: #1 tags after filtering in treatment: 19235565 INFO @ Sat, 15 Jan 2022 18:26:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:26:14: #1 finished! INFO @ Sat, 15 Jan 2022 18:26:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:26:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:26:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:26:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:26:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101448/SRX10101448.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling