Job ID = 14519933 SRX = SRX10101447 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 9112545 spots for SRR13712741/SRR13712741.sra Written 9112545 spots for SRR13712741/SRR13712741.sra Read 7604854 spots for SRR13712742/SRR13712742.sra Written 7604854 spots for SRR13712742/SRR13712742.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:18 16717399 reads; of these: 16717399 (100.00%) were unpaired; of these: 706061 (4.22%) aligned 0 times 13201387 (78.97%) aligned exactly 1 time 2809951 (16.81%) aligned >1 times 95.78% overall alignment rate Time searching: 00:02:18 Overall time: 00:02:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6213705 / 16011338 = 0.3881 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:09:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:09:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:09:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:09:15: 1000000 INFO @ Sat, 15 Jan 2022 18:09:22: 2000000 INFO @ Sat, 15 Jan 2022 18:09:28: 3000000 INFO @ Sat, 15 Jan 2022 18:09:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:09:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:09:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:09:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:09:41: 5000000 INFO @ Sat, 15 Jan 2022 18:09:45: 1000000 INFO @ Sat, 15 Jan 2022 18:09:48: 6000000 INFO @ Sat, 15 Jan 2022 18:09:51: 2000000 INFO @ Sat, 15 Jan 2022 18:09:54: 7000000 INFO @ Sat, 15 Jan 2022 18:09:57: 3000000 INFO @ Sat, 15 Jan 2022 18:10:00: 8000000 INFO @ Sat, 15 Jan 2022 18:10:03: 4000000 INFO @ Sat, 15 Jan 2022 18:10:06: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:10:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:10:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:10:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:10:09: 5000000 INFO @ Sat, 15 Jan 2022 18:10:11: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:10:11: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:10:11: #1 total tags in treatment: 9797633 INFO @ Sat, 15 Jan 2022 18:10:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:10:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:10:11: #1 tags after filtering in treatment: 9797633 INFO @ Sat, 15 Jan 2022 18:10:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:10:11: #1 finished! INFO @ Sat, 15 Jan 2022 18:10:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:10:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:10:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:10:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:10:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:10:15: 1000000 INFO @ Sat, 15 Jan 2022 18:10:15: 6000000 INFO @ Sat, 15 Jan 2022 18:10:21: 2000000 INFO @ Sat, 15 Jan 2022 18:10:21: 7000000 INFO @ Sat, 15 Jan 2022 18:10:27: 3000000 INFO @ Sat, 15 Jan 2022 18:10:27: 8000000 INFO @ Sat, 15 Jan 2022 18:10:32: 4000000 INFO @ Sat, 15 Jan 2022 18:10:33: 9000000 INFO @ Sat, 15 Jan 2022 18:10:37: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:10:37: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:10:37: #1 total tags in treatment: 9797633 INFO @ Sat, 15 Jan 2022 18:10:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:10:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:10:37: #1 tags after filtering in treatment: 9797633 INFO @ Sat, 15 Jan 2022 18:10:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:10:37: #1 finished! INFO @ Sat, 15 Jan 2022 18:10:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:10:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:10:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:10:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:10:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:10:38: 5000000 INFO @ Sat, 15 Jan 2022 18:10:43: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:10:49: 7000000 INFO @ Sat, 15 Jan 2022 18:10:54: 8000000 INFO @ Sat, 15 Jan 2022 18:10:59: 9000000 INFO @ Sat, 15 Jan 2022 18:11:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:11:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:11:03: #1 total tags in treatment: 9797633 INFO @ Sat, 15 Jan 2022 18:11:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:11:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:11:03: #1 tags after filtering in treatment: 9797633 INFO @ Sat, 15 Jan 2022 18:11:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:11:03: #1 finished! INFO @ Sat, 15 Jan 2022 18:11:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:11:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:11:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:11:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:11:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101447/SRX10101447.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling