Job ID = 14519931 SRX = SRX10101446 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7114662 spots for SRR13712739/SRR13712739.sra Written 7114662 spots for SRR13712739/SRR13712739.sra Read 7892054 spots for SRR13712740/SRR13712740.sra Written 7892054 spots for SRR13712740/SRR13712740.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:50 15006716 reads; of these: 15006716 (100.00%) were unpaired; of these: 1651496 (11.01%) aligned 0 times 10683748 (71.19%) aligned exactly 1 time 2671472 (17.80%) aligned >1 times 88.99% overall alignment rate Time searching: 00:01:50 Overall time: 00:01:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5242793 / 13355220 = 0.3926 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:07:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:07:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:07:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:08:05: 1000000 INFO @ Sat, 15 Jan 2022 18:08:10: 2000000 INFO @ Sat, 15 Jan 2022 18:08:16: 3000000 INFO @ Sat, 15 Jan 2022 18:08:21: 4000000 INFO @ Sat, 15 Jan 2022 18:08:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:08:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:08:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:08:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:08:33: 6000000 INFO @ Sat, 15 Jan 2022 18:08:36: 1000000 INFO @ Sat, 15 Jan 2022 18:08:39: 7000000 INFO @ Sat, 15 Jan 2022 18:08:43: 2000000 INFO @ Sat, 15 Jan 2022 18:08:46: 8000000 INFO @ Sat, 15 Jan 2022 18:08:47: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:08:47: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:08:47: #1 total tags in treatment: 8112427 INFO @ Sat, 15 Jan 2022 18:08:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:08:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:08:47: #1 tags after filtering in treatment: 8112427 INFO @ Sat, 15 Jan 2022 18:08:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:08:47: #1 finished! INFO @ Sat, 15 Jan 2022 18:08:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:08:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:08:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:08:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:08:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:08:49: 3000000 INFO @ Sat, 15 Jan 2022 18:08:55: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:08:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:08:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:08:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:09:00: 5000000 INFO @ Sat, 15 Jan 2022 18:09:06: 1000000 INFO @ Sat, 15 Jan 2022 18:09:07: 6000000 INFO @ Sat, 15 Jan 2022 18:09:13: 2000000 INFO @ Sat, 15 Jan 2022 18:09:14: 7000000 INFO @ Sat, 15 Jan 2022 18:09:19: 3000000 INFO @ Sat, 15 Jan 2022 18:09:20: 8000000 INFO @ Sat, 15 Jan 2022 18:09:21: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:09:21: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:09:21: #1 total tags in treatment: 8112427 INFO @ Sat, 15 Jan 2022 18:09:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:09:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:09:21: #1 tags after filtering in treatment: 8112427 INFO @ Sat, 15 Jan 2022 18:09:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:09:21: #1 finished! INFO @ Sat, 15 Jan 2022 18:09:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:09:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:09:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:09:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:09:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:09:25: 4000000 INFO @ Sat, 15 Jan 2022 18:09:31: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:09:36: 6000000 INFO @ Sat, 15 Jan 2022 18:09:42: 7000000 INFO @ Sat, 15 Jan 2022 18:09:47: 8000000 INFO @ Sat, 15 Jan 2022 18:09:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:09:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:09:48: #1 total tags in treatment: 8112427 INFO @ Sat, 15 Jan 2022 18:09:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:09:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:09:48: #1 tags after filtering in treatment: 8112427 INFO @ Sat, 15 Jan 2022 18:09:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:09:48: #1 finished! INFO @ Sat, 15 Jan 2022 18:09:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:09:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:09:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:09:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:09:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101446/SRX10101446.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling