Job ID = 14519927 SRX = SRX10101442 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7605432 spots for SRR13712731/SRR13712731.sra Written 7605432 spots for SRR13712731/SRR13712731.sra Read 9604553 spots for SRR13712732/SRR13712732.sra Written 9604553 spots for SRR13712732/SRR13712732.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:50 17209985 reads; of these: 17209985 (100.00%) were unpaired; of these: 708090 (4.11%) aligned 0 times 14469399 (84.08%) aligned exactly 1 time 2032496 (11.81%) aligned >1 times 95.89% overall alignment rate Time searching: 00:02:51 Overall time: 00:02:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5973316 / 16501895 = 0.3620 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:10:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:10:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:10:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:10:57: 1000000 INFO @ Sat, 15 Jan 2022 18:11:04: 2000000 INFO @ Sat, 15 Jan 2022 18:11:11: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:11:18: 4000000 INFO @ Sat, 15 Jan 2022 18:11:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:11:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:11:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:11:26: 5000000 INFO @ Sat, 15 Jan 2022 18:11:28: 1000000 INFO @ Sat, 15 Jan 2022 18:11:33: 6000000 INFO @ Sat, 15 Jan 2022 18:11:35: 2000000 INFO @ Sat, 15 Jan 2022 18:11:40: 7000000 INFO @ Sat, 15 Jan 2022 18:11:42: 3000000 INFO @ Sat, 15 Jan 2022 18:11:47: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:11:49: 4000000 INFO @ Sat, 15 Jan 2022 18:11:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:11:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:11:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:11:54: 9000000 INFO @ Sat, 15 Jan 2022 18:11:56: 5000000 INFO @ Sat, 15 Jan 2022 18:11:58: 1000000 INFO @ Sat, 15 Jan 2022 18:12:01: 10000000 INFO @ Sat, 15 Jan 2022 18:12:03: 6000000 INFO @ Sat, 15 Jan 2022 18:12:05: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:12:05: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:12:05: #1 total tags in treatment: 10528579 INFO @ Sat, 15 Jan 2022 18:12:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:12:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:12:05: 2000000 INFO @ Sat, 15 Jan 2022 18:12:05: #1 tags after filtering in treatment: 10528579 INFO @ Sat, 15 Jan 2022 18:12:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:12:05: #1 finished! INFO @ Sat, 15 Jan 2022 18:12:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:12:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:12:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:12:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:12:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:12:11: 7000000 INFO @ Sat, 15 Jan 2022 18:12:12: 3000000 INFO @ Sat, 15 Jan 2022 18:12:18: 8000000 INFO @ Sat, 15 Jan 2022 18:12:19: 4000000 INFO @ Sat, 15 Jan 2022 18:12:25: 9000000 INFO @ Sat, 15 Jan 2022 18:12:26: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:12:32: 10000000 INFO @ Sat, 15 Jan 2022 18:12:33: 6000000 INFO @ Sat, 15 Jan 2022 18:12:35: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:12:35: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:12:35: #1 total tags in treatment: 10528579 INFO @ Sat, 15 Jan 2022 18:12:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:12:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:12:36: #1 tags after filtering in treatment: 10528579 INFO @ Sat, 15 Jan 2022 18:12:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:12:36: #1 finished! INFO @ Sat, 15 Jan 2022 18:12:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:12:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:12:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:12:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:12:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:12:40: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:12:47: 8000000 INFO @ Sat, 15 Jan 2022 18:12:54: 9000000 INFO @ Sat, 15 Jan 2022 18:13:01: 10000000 INFO @ Sat, 15 Jan 2022 18:13:05: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:13:05: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:13:05: #1 total tags in treatment: 10528579 INFO @ Sat, 15 Jan 2022 18:13:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:13:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:13:05: #1 tags after filtering in treatment: 10528579 INFO @ Sat, 15 Jan 2022 18:13:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:13:05: #1 finished! INFO @ Sat, 15 Jan 2022 18:13:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:13:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:13:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:13:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:13:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101442/SRX10101442.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling