Job ID = 14519926 SRX = SRX10101441 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 9903424 spots for SRR13712727/SRR13712727.sra Written 9903424 spots for SRR13712727/SRR13712727.sra Read 10038765 spots for SRR13712728/SRR13712728.sra Written 10038765 spots for SRR13712728/SRR13712728.sra Read 14887883 spots for SRR13712729/SRR13712729.sra Written 14887883 spots for SRR13712729/SRR13712729.sra Read 10555845 spots for SRR13712730/SRR13712730.sra Written 10555845 spots for SRR13712730/SRR13712730.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:28 45385917 reads; of these: 45385917 (100.00%) were unpaired; of these: 3389565 (7.47%) aligned 0 times 34151968 (75.25%) aligned exactly 1 time 7844384 (17.28%) aligned >1 times 92.53% overall alignment rate Time searching: 00:05:28 Overall time: 00:05:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 24049665 / 41996352 = 0.5727 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:18:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:18:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:18:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:18:13: 1000000 INFO @ Sat, 15 Jan 2022 18:18:19: 2000000 INFO @ Sat, 15 Jan 2022 18:18:25: 3000000 INFO @ Sat, 15 Jan 2022 18:18:31: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:18:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:18:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:18:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:18:37: 5000000 INFO @ Sat, 15 Jan 2022 18:18:43: 1000000 INFO @ Sat, 15 Jan 2022 18:18:44: 6000000 INFO @ Sat, 15 Jan 2022 18:18:49: 2000000 INFO @ Sat, 15 Jan 2022 18:18:51: 7000000 INFO @ Sat, 15 Jan 2022 18:18:55: 3000000 INFO @ Sat, 15 Jan 2022 18:18:58: 8000000 INFO @ Sat, 15 Jan 2022 18:19:02: 4000000 INFO @ Sat, 15 Jan 2022 18:19:04: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:19:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:19:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:19:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:19:08: 5000000 INFO @ Sat, 15 Jan 2022 18:19:11: 10000000 INFO @ Sat, 15 Jan 2022 18:19:13: 1000000 INFO @ Sat, 15 Jan 2022 18:19:14: 6000000 INFO @ Sat, 15 Jan 2022 18:19:18: 11000000 INFO @ Sat, 15 Jan 2022 18:19:19: 2000000 INFO @ Sat, 15 Jan 2022 18:19:20: 7000000 INFO @ Sat, 15 Jan 2022 18:19:25: 12000000 INFO @ Sat, 15 Jan 2022 18:19:25: 3000000 INFO @ Sat, 15 Jan 2022 18:19:27: 8000000 INFO @ Sat, 15 Jan 2022 18:19:31: 4000000 INFO @ Sat, 15 Jan 2022 18:19:31: 13000000 INFO @ Sat, 15 Jan 2022 18:19:33: 9000000 INFO @ Sat, 15 Jan 2022 18:19:38: 5000000 INFO @ Sat, 15 Jan 2022 18:19:38: 14000000 INFO @ Sat, 15 Jan 2022 18:19:39: 10000000 INFO @ Sat, 15 Jan 2022 18:19:44: 6000000 INFO @ Sat, 15 Jan 2022 18:19:45: 15000000 INFO @ Sat, 15 Jan 2022 18:19:45: 11000000 INFO @ Sat, 15 Jan 2022 18:19:50: 7000000 INFO @ Sat, 15 Jan 2022 18:19:52: 12000000 INFO @ Sat, 15 Jan 2022 18:19:52: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:19:56: 8000000 INFO @ Sat, 15 Jan 2022 18:19:58: 13000000 INFO @ Sat, 15 Jan 2022 18:19:59: 17000000 INFO @ Sat, 15 Jan 2022 18:20:02: 9000000 INFO @ Sat, 15 Jan 2022 18:20:04: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:20:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:20:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:20:06: #1 total tags in treatment: 17946687 INFO @ Sat, 15 Jan 2022 18:20:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:20:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:20:06: #1 tags after filtering in treatment: 17946687 INFO @ Sat, 15 Jan 2022 18:20:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:20:06: #1 finished! INFO @ Sat, 15 Jan 2022 18:20:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:20:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:20:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:20:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:20:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:20:09: 10000000 INFO @ Sat, 15 Jan 2022 18:20:10: 15000000 INFO @ Sat, 15 Jan 2022 18:20:15: 11000000 INFO @ Sat, 15 Jan 2022 18:20:16: 16000000 INFO @ Sat, 15 Jan 2022 18:20:21: 12000000 INFO @ Sat, 15 Jan 2022 18:20:22: 17000000 INFO @ Sat, 15 Jan 2022 18:20:27: 13000000 INFO @ Sat, 15 Jan 2022 18:20:28: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:20:28: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:20:28: #1 total tags in treatment: 17946687 INFO @ Sat, 15 Jan 2022 18:20:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:20:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:20:28: #1 tags after filtering in treatment: 17946687 INFO @ Sat, 15 Jan 2022 18:20:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:20:28: #1 finished! INFO @ Sat, 15 Jan 2022 18:20:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:20:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:20:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:20:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:20:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:20:33: 14000000 INFO @ Sat, 15 Jan 2022 18:20:38: 15000000 INFO @ Sat, 15 Jan 2022 18:20:44: 16000000 INFO @ Sat, 15 Jan 2022 18:20:49: 17000000 INFO @ Sat, 15 Jan 2022 18:20:55: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:20:55: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:20:55: #1 total tags in treatment: 17946687 INFO @ Sat, 15 Jan 2022 18:20:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:20:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:20:55: #1 tags after filtering in treatment: 17946687 INFO @ Sat, 15 Jan 2022 18:20:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:20:55: #1 finished! INFO @ Sat, 15 Jan 2022 18:20:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:20:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:20:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:20:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:20:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101441/SRX10101441.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling