Job ID = 14519925 SRX = SRX10101440 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10260025 spots for SRR13712723/SRR13712723.sra Written 10260025 spots for SRR13712723/SRR13712723.sra Read 6291795 spots for SRR13712724/SRR13712724.sra Written 6291795 spots for SRR13712724/SRR13712724.sra Read 5192449 spots for SRR13712725/SRR13712725.sra Written 5192449 spots for SRR13712725/SRR13712725.sra Read 7010211 spots for SRR13712726/SRR13712726.sra Written 7010211 spots for SRR13712726/SRR13712726.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:47 28754480 reads; of these: 28754480 (100.00%) were unpaired; of these: 1647329 (5.73%) aligned 0 times 23241717 (80.83%) aligned exactly 1 time 3865434 (13.44%) aligned >1 times 94.27% overall alignment rate Time searching: 00:03:47 Overall time: 00:03:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12811366 / 27107151 = 0.4726 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:12:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:12:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:12:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:12:36: 1000000 INFO @ Sat, 15 Jan 2022 18:12:41: 2000000 INFO @ Sat, 15 Jan 2022 18:12:46: 3000000 INFO @ Sat, 15 Jan 2022 18:12:51: 4000000 INFO @ Sat, 15 Jan 2022 18:12:56: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:13:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:13:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:13:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:13:02: 6000000 INFO @ Sat, 15 Jan 2022 18:13:05: 1000000 INFO @ Sat, 15 Jan 2022 18:13:08: 7000000 INFO @ Sat, 15 Jan 2022 18:13:11: 2000000 INFO @ Sat, 15 Jan 2022 18:13:14: 8000000 INFO @ Sat, 15 Jan 2022 18:13:17: 3000000 INFO @ Sat, 15 Jan 2022 18:13:20: 9000000 INFO @ Sat, 15 Jan 2022 18:13:23: 4000000 INFO @ Sat, 15 Jan 2022 18:13:26: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:13:29: 5000000 INFO @ Sat, 15 Jan 2022 18:13:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:13:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:13:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:13:32: 11000000 INFO @ Sat, 15 Jan 2022 18:13:35: 6000000 INFO @ Sat, 15 Jan 2022 18:13:36: 1000000 INFO @ Sat, 15 Jan 2022 18:13:39: 12000000 INFO @ Sat, 15 Jan 2022 18:13:41: 7000000 INFO @ Sat, 15 Jan 2022 18:13:43: 2000000 INFO @ Sat, 15 Jan 2022 18:13:45: 13000000 INFO @ Sat, 15 Jan 2022 18:13:47: 8000000 INFO @ Sat, 15 Jan 2022 18:13:50: 3000000 INFO @ Sat, 15 Jan 2022 18:13:51: 14000000 INFO @ Sat, 15 Jan 2022 18:13:53: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:13:53: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:13:53: #1 total tags in treatment: 14295785 INFO @ Sat, 15 Jan 2022 18:13:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:13:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:13:53: 9000000 INFO @ Sat, 15 Jan 2022 18:13:53: #1 tags after filtering in treatment: 14295785 INFO @ Sat, 15 Jan 2022 18:13:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:13:53: #1 finished! INFO @ Sat, 15 Jan 2022 18:13:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:13:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:13:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:13:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:13:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:13:56: 4000000 INFO @ Sat, 15 Jan 2022 18:14:00: 10000000 INFO @ Sat, 15 Jan 2022 18:14:03: 5000000 INFO @ Sat, 15 Jan 2022 18:14:07: 11000000 INFO @ Sat, 15 Jan 2022 18:14:09: 6000000 INFO @ Sat, 15 Jan 2022 18:14:12: 12000000 INFO @ Sat, 15 Jan 2022 18:14:15: 7000000 INFO @ Sat, 15 Jan 2022 18:14:18: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:14:21: 8000000 INFO @ Sat, 15 Jan 2022 18:14:24: 14000000 INFO @ Sat, 15 Jan 2022 18:14:26: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:14:26: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:14:26: #1 total tags in treatment: 14295785 INFO @ Sat, 15 Jan 2022 18:14:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:14:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:14:26: #1 tags after filtering in treatment: 14295785 INFO @ Sat, 15 Jan 2022 18:14:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:14:26: #1 finished! INFO @ Sat, 15 Jan 2022 18:14:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:14:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:14:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:14:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:14:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:14:28: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:14:33: 10000000 INFO @ Sat, 15 Jan 2022 18:14:38: 11000000 INFO @ Sat, 15 Jan 2022 18:14:44: 12000000 INFO @ Sat, 15 Jan 2022 18:14:49: 13000000 INFO @ Sat, 15 Jan 2022 18:14:55: 14000000 INFO @ Sat, 15 Jan 2022 18:14:57: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:14:57: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:14:57: #1 total tags in treatment: 14295785 INFO @ Sat, 15 Jan 2022 18:14:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:14:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:14:57: #1 tags after filtering in treatment: 14295785 INFO @ Sat, 15 Jan 2022 18:14:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:14:57: #1 finished! INFO @ Sat, 15 Jan 2022 18:14:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:14:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:14:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:14:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:14:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101440/SRX10101440.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling