Job ID = 14519921 SRX = SRX10101439 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6179667 spots for SRR13712719/SRR13712719.sra Written 6179667 spots for SRR13712719/SRR13712719.sra Read 6834338 spots for SRR13712720/SRR13712720.sra Written 6834338 spots for SRR13712720/SRR13712720.sra Read 8392811 spots for SRR13712721/SRR13712721.sra Written 8392811 spots for SRR13712721/SRR13712721.sra Read 7112241 spots for SRR13712722/SRR13712722.sra Written 7112241 spots for SRR13712722/SRR13712722.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:49 28519057 reads; of these: 28519057 (100.00%) were unpaired; of these: 1500193 (5.26%) aligned 0 times 23267297 (81.59%) aligned exactly 1 time 3751567 (13.15%) aligned >1 times 94.74% overall alignment rate Time searching: 00:03:49 Overall time: 00:03:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12810790 / 27018864 = 0.4741 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:12:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:12:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:12:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:12:34: 1000000 INFO @ Sat, 15 Jan 2022 18:12:40: 2000000 INFO @ Sat, 15 Jan 2022 18:12:46: 3000000 INFO @ Sat, 15 Jan 2022 18:12:52: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:12:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:12:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:12:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:12:58: 5000000 INFO @ Sat, 15 Jan 2022 18:13:03: 1000000 INFO @ Sat, 15 Jan 2022 18:13:04: 6000000 INFO @ Sat, 15 Jan 2022 18:13:09: 2000000 INFO @ Sat, 15 Jan 2022 18:13:11: 7000000 INFO @ Sat, 15 Jan 2022 18:13:16: 3000000 INFO @ Sat, 15 Jan 2022 18:13:17: 8000000 INFO @ Sat, 15 Jan 2022 18:13:22: 4000000 INFO @ Sat, 15 Jan 2022 18:13:24: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:13:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:13:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:13:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:13:28: 5000000 INFO @ Sat, 15 Jan 2022 18:13:30: 10000000 INFO @ Sat, 15 Jan 2022 18:13:33: 1000000 INFO @ Sat, 15 Jan 2022 18:13:34: 6000000 INFO @ Sat, 15 Jan 2022 18:13:36: 11000000 INFO @ Sat, 15 Jan 2022 18:13:40: 2000000 INFO @ Sat, 15 Jan 2022 18:13:40: 7000000 INFO @ Sat, 15 Jan 2022 18:13:43: 12000000 INFO @ Sat, 15 Jan 2022 18:13:46: 3000000 INFO @ Sat, 15 Jan 2022 18:13:46: 8000000 INFO @ Sat, 15 Jan 2022 18:13:49: 13000000 INFO @ Sat, 15 Jan 2022 18:13:52: 4000000 INFO @ Sat, 15 Jan 2022 18:13:52: 9000000 INFO @ Sat, 15 Jan 2022 18:13:56: 14000000 INFO @ Sat, 15 Jan 2022 18:13:57: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:13:57: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:13:57: #1 total tags in treatment: 14208074 INFO @ Sat, 15 Jan 2022 18:13:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:13:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:13:57: #1 tags after filtering in treatment: 14208074 INFO @ Sat, 15 Jan 2022 18:13:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:13:57: #1 finished! INFO @ Sat, 15 Jan 2022 18:13:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:13:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:13:58: 5000000 INFO @ Sat, 15 Jan 2022 18:13:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:13:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:13:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:13:58: 10000000 INFO @ Sat, 15 Jan 2022 18:14:03: 6000000 INFO @ Sat, 15 Jan 2022 18:14:04: 11000000 INFO @ Sat, 15 Jan 2022 18:14:09: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:14:10: 12000000 INFO @ Sat, 15 Jan 2022 18:14:15: 8000000 INFO @ Sat, 15 Jan 2022 18:14:16: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:14:21: 9000000 INFO @ Sat, 15 Jan 2022 18:14:22: 14000000 INFO @ Sat, 15 Jan 2022 18:14:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:14:23: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:14:23: #1 total tags in treatment: 14208074 INFO @ Sat, 15 Jan 2022 18:14:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:14:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:14:23: #1 tags after filtering in treatment: 14208074 INFO @ Sat, 15 Jan 2022 18:14:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:14:23: #1 finished! INFO @ Sat, 15 Jan 2022 18:14:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:14:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:14:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:14:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:14:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:14:26: 10000000 INFO @ Sat, 15 Jan 2022 18:14:32: 11000000 INFO @ Sat, 15 Jan 2022 18:14:37: 12000000 INFO @ Sat, 15 Jan 2022 18:14:43: 13000000 INFO @ Sat, 15 Jan 2022 18:14:48: 14000000 INFO @ Sat, 15 Jan 2022 18:14:49: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:14:49: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:14:49: #1 total tags in treatment: 14208074 INFO @ Sat, 15 Jan 2022 18:14:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:14:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:14:50: #1 tags after filtering in treatment: 14208074 INFO @ Sat, 15 Jan 2022 18:14:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:14:50: #1 finished! INFO @ Sat, 15 Jan 2022 18:14:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:14:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:14:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:14:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:14:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101439/SRX10101439.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling