Job ID = 14519920 SRX = SRX10101438 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5987158 spots for SRR13712715/SRR13712715.sra Written 5987158 spots for SRR13712715/SRR13712715.sra Read 9800243 spots for SRR13712716/SRR13712716.sra Written 9800243 spots for SRR13712716/SRR13712716.sra Read 11952139 spots for SRR13712717/SRR13712717.sra Written 11952139 spots for SRR13712717/SRR13712717.sra Read 7889074 spots for SRR13712718/SRR13712718.sra Written 7889074 spots for SRR13712718/SRR13712718.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:26 35628614 reads; of these: 35628614 (100.00%) were unpaired; of these: 1402873 (3.94%) aligned 0 times 28796492 (80.82%) aligned exactly 1 time 5429249 (15.24%) aligned >1 times 96.06% overall alignment rate Time searching: 00:05:26 Overall time: 00:05:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18612121 / 34225741 = 0.5438 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:17:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:17:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:17:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:17:35: 1000000 INFO @ Sat, 15 Jan 2022 18:17:44: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:17:54: 3000000 INFO @ Sat, 15 Jan 2022 18:17:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:17:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:17:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:18:03: 4000000 INFO @ Sat, 15 Jan 2022 18:18:05: 1000000 INFO @ Sat, 15 Jan 2022 18:18:12: 5000000 INFO @ Sat, 15 Jan 2022 18:18:15: 2000000 INFO @ Sat, 15 Jan 2022 18:18:22: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:18:24: 3000000 INFO @ Sat, 15 Jan 2022 18:18:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:18:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:18:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:18:32: 7000000 INFO @ Sat, 15 Jan 2022 18:18:34: 4000000 INFO @ Sat, 15 Jan 2022 18:18:34: 1000000 INFO @ Sat, 15 Jan 2022 18:18:41: 8000000 INFO @ Sat, 15 Jan 2022 18:18:43: 2000000 INFO @ Sat, 15 Jan 2022 18:18:44: 5000000 INFO @ Sat, 15 Jan 2022 18:18:51: 3000000 INFO @ Sat, 15 Jan 2022 18:18:51: 9000000 INFO @ Sat, 15 Jan 2022 18:18:53: 6000000 INFO @ Sat, 15 Jan 2022 18:18:59: 4000000 INFO @ Sat, 15 Jan 2022 18:19:00: 10000000 INFO @ Sat, 15 Jan 2022 18:19:03: 7000000 INFO @ Sat, 15 Jan 2022 18:19:07: 5000000 INFO @ Sat, 15 Jan 2022 18:19:10: 11000000 INFO @ Sat, 15 Jan 2022 18:19:13: 8000000 INFO @ Sat, 15 Jan 2022 18:19:15: 6000000 INFO @ Sat, 15 Jan 2022 18:19:20: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:19:22: 9000000 INFO @ Sat, 15 Jan 2022 18:19:23: 7000000 INFO @ Sat, 15 Jan 2022 18:19:30: 13000000 INFO @ Sat, 15 Jan 2022 18:19:31: 8000000 INFO @ Sat, 15 Jan 2022 18:19:32: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:19:40: 14000000 INFO @ Sat, 15 Jan 2022 18:19:41: 9000000 INFO @ Sat, 15 Jan 2022 18:19:42: 11000000 INFO @ Sat, 15 Jan 2022 18:19:49: 10000000 INFO @ Sat, 15 Jan 2022 18:19:49: 15000000 INFO @ Sat, 15 Jan 2022 18:19:52: 12000000 INFO @ Sat, 15 Jan 2022 18:19:55: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:19:55: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:19:55: #1 total tags in treatment: 15613620 INFO @ Sat, 15 Jan 2022 18:19:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:19:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:19:55: #1 tags after filtering in treatment: 15613620 INFO @ Sat, 15 Jan 2022 18:19:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:19:55: #1 finished! INFO @ Sat, 15 Jan 2022 18:19:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:19:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:19:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:19:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:19:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:19:58: 11000000 INFO @ Sat, 15 Jan 2022 18:20:02: 13000000 INFO @ Sat, 15 Jan 2022 18:20:06: 12000000 INFO @ Sat, 15 Jan 2022 18:20:11: 14000000 INFO @ Sat, 15 Jan 2022 18:20:15: 13000000 INFO @ Sat, 15 Jan 2022 18:20:21: 15000000 INFO @ Sat, 15 Jan 2022 18:20:24: 14000000 INFO @ Sat, 15 Jan 2022 18:20:26: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:20:26: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:20:26: #1 total tags in treatment: 15613620 INFO @ Sat, 15 Jan 2022 18:20:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:20:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:20:27: #1 tags after filtering in treatment: 15613620 INFO @ Sat, 15 Jan 2022 18:20:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:20:27: #1 finished! INFO @ Sat, 15 Jan 2022 18:20:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:20:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:20:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:20:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:20:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:20:32: 15000000 INFO @ Sat, 15 Jan 2022 18:20:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:20:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:20:36: #1 total tags in treatment: 15613620 INFO @ Sat, 15 Jan 2022 18:20:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:20:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:20:37: #1 tags after filtering in treatment: 15613620 INFO @ Sat, 15 Jan 2022 18:20:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:20:37: #1 finished! INFO @ Sat, 15 Jan 2022 18:20:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:20:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:20:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:20:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:20:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101438/SRX10101438.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling