Job ID = 14519917 SRX = SRX10101437 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13426457 spots for SRR13712711/SRR13712711.sra Written 13426457 spots for SRR13712711/SRR13712711.sra Read 13421731 spots for SRR13712712/SRR13712712.sra Written 13421731 spots for SRR13712712/SRR13712712.sra Read 13730745 spots for SRR13712713/SRR13712713.sra Written 13730745 spots for SRR13712713/SRR13712713.sra Read 14878848 spots for SRR13712714/SRR13712714.sra Written 14878848 spots for SRR13712714/SRR13712714.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:33 55457781 reads; of these: 55457781 (100.00%) were unpaired; of these: 2878995 (5.19%) aligned 0 times 43309073 (78.09%) aligned exactly 1 time 9269713 (16.71%) aligned >1 times 94.81% overall alignment rate Time searching: 00:06:33 Overall time: 00:06:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 33066210 / 52578786 = 0.6289 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:19:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:19:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:19:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:19:58: 1000000 INFO @ Sat, 15 Jan 2022 18:20:03: 2000000 INFO @ Sat, 15 Jan 2022 18:20:08: 3000000 INFO @ Sat, 15 Jan 2022 18:20:13: 4000000 INFO @ Sat, 15 Jan 2022 18:20:18: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:20:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:20:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:20:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:20:23: 6000000 INFO @ Sat, 15 Jan 2022 18:20:28: 1000000 INFO @ Sat, 15 Jan 2022 18:20:29: 7000000 INFO @ Sat, 15 Jan 2022 18:20:34: 2000000 INFO @ Sat, 15 Jan 2022 18:20:35: 8000000 INFO @ Sat, 15 Jan 2022 18:20:40: 3000000 INFO @ Sat, 15 Jan 2022 18:20:41: 9000000 INFO @ Sat, 15 Jan 2022 18:20:46: 4000000 INFO @ Sat, 15 Jan 2022 18:20:46: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:20:51: 5000000 INFO @ Sat, 15 Jan 2022 18:20:52: 11000000 INFO @ Sat, 15 Jan 2022 18:20:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:20:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:20:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:20:57: 6000000 INFO @ Sat, 15 Jan 2022 18:20:58: 12000000 INFO @ Sat, 15 Jan 2022 18:20:59: 1000000 INFO @ Sat, 15 Jan 2022 18:21:03: 7000000 INFO @ Sat, 15 Jan 2022 18:21:04: 13000000 INFO @ Sat, 15 Jan 2022 18:21:05: 2000000 INFO @ Sat, 15 Jan 2022 18:21:09: 8000000 INFO @ Sat, 15 Jan 2022 18:21:09: 14000000 INFO @ Sat, 15 Jan 2022 18:21:11: 3000000 INFO @ Sat, 15 Jan 2022 18:21:15: 9000000 INFO @ Sat, 15 Jan 2022 18:21:15: 15000000 INFO @ Sat, 15 Jan 2022 18:21:17: 4000000 INFO @ Sat, 15 Jan 2022 18:21:21: 10000000 INFO @ Sat, 15 Jan 2022 18:21:21: 16000000 INFO @ Sat, 15 Jan 2022 18:21:23: 5000000 INFO @ Sat, 15 Jan 2022 18:21:26: 11000000 INFO @ Sat, 15 Jan 2022 18:21:27: 17000000 INFO @ Sat, 15 Jan 2022 18:21:29: 6000000 INFO @ Sat, 15 Jan 2022 18:21:32: 12000000 INFO @ Sat, 15 Jan 2022 18:21:33: 18000000 INFO @ Sat, 15 Jan 2022 18:21:35: 7000000 INFO @ Sat, 15 Jan 2022 18:21:38: 13000000 INFO @ Sat, 15 Jan 2022 18:21:38: 19000000 INFO @ Sat, 15 Jan 2022 18:21:41: 8000000 INFO @ Sat, 15 Jan 2022 18:21:41: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:21:41: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:21:41: #1 total tags in treatment: 19512576 INFO @ Sat, 15 Jan 2022 18:21:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:21:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:21:42: #1 tags after filtering in treatment: 19512576 INFO @ Sat, 15 Jan 2022 18:21:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:21:42: #1 finished! INFO @ Sat, 15 Jan 2022 18:21:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:21:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:21:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:21:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:21:43: Process for pairing-model is terminated! BedGraph に変換しました。 BigWig に変換中... cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:21:44: 14000000 INFO @ Sat, 15 Jan 2022 18:21:47: 9000000 INFO @ Sat, 15 Jan 2022 18:21:50: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:21:53: 10000000 INFO @ Sat, 15 Jan 2022 18:21:55: 16000000 INFO @ Sat, 15 Jan 2022 18:21:59: 11000000 INFO @ Sat, 15 Jan 2022 18:22:01: 17000000 INFO @ Sat, 15 Jan 2022 18:22:05: 12000000 INFO @ Sat, 15 Jan 2022 18:22:07: 18000000 INFO @ Sat, 15 Jan 2022 18:22:10: 13000000 INFO @ Sat, 15 Jan 2022 18:22:13: 19000000 INFO @ Sat, 15 Jan 2022 18:22:16: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:22:16: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:22:16: #1 total tags in treatment: 19512576 INFO @ Sat, 15 Jan 2022 18:22:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:22:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:22:16: #1 tags after filtering in treatment: 19512576 INFO @ Sat, 15 Jan 2022 18:22:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:22:16: #1 finished! INFO @ Sat, 15 Jan 2022 18:22:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:22:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:22:16: 14000000 INFO @ Sat, 15 Jan 2022 18:22:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:22:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:22:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:22:22: 15000000 INFO @ Sat, 15 Jan 2022 18:22:27: 16000000 INFO @ Sat, 15 Jan 2022 18:22:33: 17000000 INFO @ Sat, 15 Jan 2022 18:22:38: 18000000 INFO @ Sat, 15 Jan 2022 18:22:43: 19000000 INFO @ Sat, 15 Jan 2022 18:22:46: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:22:46: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:22:46: #1 total tags in treatment: 19512576 INFO @ Sat, 15 Jan 2022 18:22:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:22:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:22:46: #1 tags after filtering in treatment: 19512576 INFO @ Sat, 15 Jan 2022 18:22:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:22:46: #1 finished! INFO @ Sat, 15 Jan 2022 18:22:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:22:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:22:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:22:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:22:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101437/SRX10101437.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling