Job ID = 14519914 SRX = SRX10101436 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13317022 spots for SRR13712707/SRR13712707.sra Written 13317022 spots for SRR13712707/SRR13712707.sra Read 15982495 spots for SRR13712708/SRR13712708.sra Written 15982495 spots for SRR13712708/SRR13712708.sra Read 11804734 spots for SRR13712709/SRR13712709.sra Written 11804734 spots for SRR13712709/SRR13712709.sra Read 14715464 spots for SRR13712710/SRR13712710.sra Written 14715464 spots for SRR13712710/SRR13712710.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:19 55819715 reads; of these: 55819715 (100.00%) were unpaired; of these: 3510537 (6.29%) aligned 0 times 42467448 (76.08%) aligned exactly 1 time 9841730 (17.63%) aligned >1 times 93.71% overall alignment rate Time searching: 00:10:19 Overall time: 00:10:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 32988375 / 52309178 = 0.6306 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:38:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:38:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:38:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:38:29: 1000000 INFO @ Sat, 15 Jan 2022 18:38:39: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:38:48: 3000000 INFO @ Sat, 15 Jan 2022 18:38:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:38:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:38:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:38:57: 4000000 INFO @ Sat, 15 Jan 2022 18:39:00: 1000000 INFO @ Sat, 15 Jan 2022 18:39:06: 5000000 INFO @ Sat, 15 Jan 2022 18:39:09: 2000000 INFO @ Sat, 15 Jan 2022 18:39:15: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:39:18: 3000000 INFO @ Sat, 15 Jan 2022 18:39:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:39:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:39:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:39:24: 7000000 INFO @ Sat, 15 Jan 2022 18:39:26: 4000000 INFO @ Sat, 15 Jan 2022 18:39:28: 1000000 INFO @ Sat, 15 Jan 2022 18:39:33: 8000000 INFO @ Sat, 15 Jan 2022 18:39:34: 5000000 INFO @ Sat, 15 Jan 2022 18:39:36: 2000000 INFO @ Sat, 15 Jan 2022 18:39:42: 6000000 INFO @ Sat, 15 Jan 2022 18:39:42: 9000000 INFO @ Sat, 15 Jan 2022 18:39:44: 3000000 INFO @ Sat, 15 Jan 2022 18:39:50: 7000000 INFO @ Sat, 15 Jan 2022 18:39:51: 10000000 INFO @ Sat, 15 Jan 2022 18:39:52: 4000000 INFO @ Sat, 15 Jan 2022 18:39:58: 8000000 INFO @ Sat, 15 Jan 2022 18:40:00: 5000000 INFO @ Sat, 15 Jan 2022 18:40:00: 11000000 INFO @ Sat, 15 Jan 2022 18:40:06: 9000000 INFO @ Sat, 15 Jan 2022 18:40:08: 6000000 INFO @ Sat, 15 Jan 2022 18:40:09: 12000000 INFO @ Sat, 15 Jan 2022 18:40:14: 10000000 INFO @ Sat, 15 Jan 2022 18:40:15: 7000000 INFO @ Sat, 15 Jan 2022 18:40:18: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:40:22: 11000000 INFO @ Sat, 15 Jan 2022 18:40:23: 8000000 INFO @ Sat, 15 Jan 2022 18:40:27: 14000000 INFO @ Sat, 15 Jan 2022 18:40:29: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:40:31: 9000000 INFO @ Sat, 15 Jan 2022 18:40:36: 15000000 INFO @ Sat, 15 Jan 2022 18:40:37: 13000000 INFO @ Sat, 15 Jan 2022 18:40:39: 10000000 INFO @ Sat, 15 Jan 2022 18:40:44: 16000000 INFO @ Sat, 15 Jan 2022 18:40:45: 14000000 INFO @ Sat, 15 Jan 2022 18:40:47: 11000000 INFO @ Sat, 15 Jan 2022 18:40:53: 15000000 INFO @ Sat, 15 Jan 2022 18:40:53: 17000000 INFO @ Sat, 15 Jan 2022 18:40:55: 12000000 INFO @ Sat, 15 Jan 2022 18:41:01: 16000000 INFO @ Sat, 15 Jan 2022 18:41:02: 18000000 INFO @ Sat, 15 Jan 2022 18:41:03: 13000000 INFO @ Sat, 15 Jan 2022 18:41:09: 17000000 INFO @ Sat, 15 Jan 2022 18:41:10: 14000000 INFO @ Sat, 15 Jan 2022 18:41:11: 19000000 INFO @ Sat, 15 Jan 2022 18:41:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:41:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:41:14: #1 total tags in treatment: 19320803 INFO @ Sat, 15 Jan 2022 18:41:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:41:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:41:14: #1 tags after filtering in treatment: 19320803 INFO @ Sat, 15 Jan 2022 18:41:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:41:14: #1 finished! INFO @ Sat, 15 Jan 2022 18:41:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:41:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:41:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:41:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:41:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:41:16: 18000000 INFO @ Sat, 15 Jan 2022 18:41:18: 15000000 INFO @ Sat, 15 Jan 2022 18:41:24: 19000000 INFO @ Sat, 15 Jan 2022 18:41:26: 16000000 INFO @ Sat, 15 Jan 2022 18:41:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:41:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:41:27: #1 total tags in treatment: 19320803 INFO @ Sat, 15 Jan 2022 18:41:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:41:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:41:27: #1 tags after filtering in treatment: 19320803 INFO @ Sat, 15 Jan 2022 18:41:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:41:27: #1 finished! INFO @ Sat, 15 Jan 2022 18:41:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:41:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:41:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:41:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:41:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:41:34: 17000000 INFO @ Sat, 15 Jan 2022 18:41:41: 18000000 INFO @ Sat, 15 Jan 2022 18:41:49: 19000000 INFO @ Sat, 15 Jan 2022 18:41:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:41:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:41:51: #1 total tags in treatment: 19320803 INFO @ Sat, 15 Jan 2022 18:41:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:41:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:41:52: #1 tags after filtering in treatment: 19320803 INFO @ Sat, 15 Jan 2022 18:41:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:41:52: #1 finished! INFO @ Sat, 15 Jan 2022 18:41:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:41:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:41:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:41:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:41:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101436/SRX10101436.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling