Job ID = 14519913 SRX = SRX10101435 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 12610373 spots for SRR13712703/SRR13712703.sra Written 12610373 spots for SRR13712703/SRR13712703.sra Read 18334253 spots for SRR13712704/SRR13712704.sra Written 18334253 spots for SRR13712704/SRR13712704.sra Read 14603953 spots for SRR13712705/SRR13712705.sra Written 14603953 spots for SRR13712705/SRR13712705.sra Read 18799052 spots for SRR13712706/SRR13712706.sra Written 18799052 spots for SRR13712706/SRR13712706.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:49 64347631 reads; of these: 64347631 (100.00%) were unpaired; of these: 5963063 (9.27%) aligned 0 times 46484950 (72.24%) aligned exactly 1 time 11899618 (18.49%) aligned >1 times 90.73% overall alignment rate Time searching: 00:07:49 Overall time: 00:07:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 38344756 / 58384568 = 0.6568 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:24:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:24:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:24:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:24:17: 1000000 INFO @ Sat, 15 Jan 2022 18:24:23: 2000000 INFO @ Sat, 15 Jan 2022 18:24:29: 3000000 INFO @ Sat, 15 Jan 2022 18:24:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:24:41: 5000000 INFO @ Sat, 15 Jan 2022 18:24:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:24:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:24:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:24:47: 6000000 INFO @ Sat, 15 Jan 2022 18:24:49: 1000000 INFO @ Sat, 15 Jan 2022 18:24:53: 7000000 INFO @ Sat, 15 Jan 2022 18:24:55: 2000000 INFO @ Sat, 15 Jan 2022 18:24:59: 8000000 INFO @ Sat, 15 Jan 2022 18:25:01: 3000000 INFO @ Sat, 15 Jan 2022 18:25:05: 9000000 INFO @ Sat, 15 Jan 2022 18:25:06: 4000000 INFO @ Sat, 15 Jan 2022 18:25:10: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:25:12: 5000000 INFO @ Sat, 15 Jan 2022 18:25:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:25:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:25:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:25:15: 11000000 INFO @ Sat, 15 Jan 2022 18:25:17: 6000000 INFO @ Sat, 15 Jan 2022 18:25:18: 1000000 INFO @ Sat, 15 Jan 2022 18:25:20: 12000000 INFO @ Sat, 15 Jan 2022 18:25:22: 7000000 INFO @ Sat, 15 Jan 2022 18:25:24: 2000000 INFO @ Sat, 15 Jan 2022 18:25:25: 13000000 INFO @ Sat, 15 Jan 2022 18:25:27: 8000000 INFO @ Sat, 15 Jan 2022 18:25:29: 3000000 INFO @ Sat, 15 Jan 2022 18:25:30: 14000000 INFO @ Sat, 15 Jan 2022 18:25:32: 9000000 INFO @ Sat, 15 Jan 2022 18:25:34: 4000000 INFO @ Sat, 15 Jan 2022 18:25:35: 15000000 INFO @ Sat, 15 Jan 2022 18:25:37: 10000000 INFO @ Sat, 15 Jan 2022 18:25:39: 5000000 INFO @ Sat, 15 Jan 2022 18:25:40: 16000000 INFO @ Sat, 15 Jan 2022 18:25:43: 11000000 INFO @ Sat, 15 Jan 2022 18:25:44: 6000000 INFO @ Sat, 15 Jan 2022 18:25:45: 17000000 INFO @ Sat, 15 Jan 2022 18:25:48: 12000000 INFO @ Sat, 15 Jan 2022 18:25:49: 7000000 INFO @ Sat, 15 Jan 2022 18:25:50: 18000000 INFO @ Sat, 15 Jan 2022 18:25:53: 13000000 INFO @ Sat, 15 Jan 2022 18:25:54: 8000000 INFO @ Sat, 15 Jan 2022 18:25:55: 19000000 INFO @ Sat, 15 Jan 2022 18:25:58: 14000000 INFO @ Sat, 15 Jan 2022 18:25:59: 9000000 INFO @ Sat, 15 Jan 2022 18:26:00: 20000000 INFO @ Sat, 15 Jan 2022 18:26:01: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:26:01: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:26:01: #1 total tags in treatment: 20039812 INFO @ Sat, 15 Jan 2022 18:26:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:26:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:26:01: #1 tags after filtering in treatment: 20039812 INFO @ Sat, 15 Jan 2022 18:26:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:26:01: #1 finished! INFO @ Sat, 15 Jan 2022 18:26:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:26:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:26:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:26:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:26:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 18:26:03: 15000000 pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:26:04: 10000000 INFO @ Sat, 15 Jan 2022 18:26:08: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:26:09: 11000000 INFO @ Sat, 15 Jan 2022 18:26:13: 17000000 INFO @ Sat, 15 Jan 2022 18:26:14: 12000000 INFO @ Sat, 15 Jan 2022 18:26:18: 18000000 INFO @ Sat, 15 Jan 2022 18:26:19: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:26:23: 19000000 INFO @ Sat, 15 Jan 2022 18:26:24: 14000000 INFO @ Sat, 15 Jan 2022 18:26:28: 20000000 INFO @ Sat, 15 Jan 2022 18:26:29: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:26:29: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:26:29: #1 total tags in treatment: 20039812 INFO @ Sat, 15 Jan 2022 18:26:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:26:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:26:29: #1 tags after filtering in treatment: 20039812 INFO @ Sat, 15 Jan 2022 18:26:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:26:29: #1 finished! INFO @ Sat, 15 Jan 2022 18:26:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:26:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:26:29: 15000000 INFO @ Sat, 15 Jan 2022 18:26:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:26:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:26:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:26:34: 16000000 INFO @ Sat, 15 Jan 2022 18:26:39: 17000000 INFO @ Sat, 15 Jan 2022 18:26:44: 18000000 INFO @ Sat, 15 Jan 2022 18:26:49: 19000000 INFO @ Sat, 15 Jan 2022 18:26:54: 20000000 INFO @ Sat, 15 Jan 2022 18:26:54: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:26:54: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:26:54: #1 total tags in treatment: 20039812 INFO @ Sat, 15 Jan 2022 18:26:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:26:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:26:54: #1 tags after filtering in treatment: 20039812 INFO @ Sat, 15 Jan 2022 18:26:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:26:54: #1 finished! INFO @ Sat, 15 Jan 2022 18:26:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:26:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:26:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:26:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:26:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101435/SRX10101435.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling