Job ID = 14519912 SRX = SRX10101434 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 16076053 spots for SRR13712699/SRR13712699.sra Written 16076053 spots for SRR13712699/SRR13712699.sra Read 18582964 spots for SRR13712700/SRR13712700.sra Written 18582964 spots for SRR13712700/SRR13712700.sra Read 7155106 spots for SRR13712701/SRR13712701.sra Written 7155106 spots for SRR13712701/SRR13712701.sra Read 11358477 spots for SRR13712702/SRR13712702.sra Written 11358477 spots for SRR13712702/SRR13712702.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:04 53172600 reads; of these: 53172600 (100.00%) were unpaired; of these: 4358806 (8.20%) aligned 0 times 40617069 (76.39%) aligned exactly 1 time 8196725 (15.42%) aligned >1 times 91.80% overall alignment rate Time searching: 00:08:04 Overall time: 00:08:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 29357010 / 48813794 = 0.6014 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:25:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:25:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:25:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:25:10: 1000000 INFO @ Sat, 15 Jan 2022 18:25:15: 2000000 INFO @ Sat, 15 Jan 2022 18:25:20: 3000000 INFO @ Sat, 15 Jan 2022 18:25:26: 4000000 INFO @ Sat, 15 Jan 2022 18:25:31: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:25:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:25:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:25:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:25:36: 6000000 INFO @ Sat, 15 Jan 2022 18:25:40: 1000000 INFO @ Sat, 15 Jan 2022 18:25:42: 7000000 INFO @ Sat, 15 Jan 2022 18:25:46: 2000000 INFO @ Sat, 15 Jan 2022 18:25:47: 8000000 INFO @ Sat, 15 Jan 2022 18:25:52: 3000000 INFO @ Sat, 15 Jan 2022 18:25:53: 9000000 INFO @ Sat, 15 Jan 2022 18:25:58: 10000000 INFO @ Sat, 15 Jan 2022 18:25:59: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:26:04: 11000000 INFO @ Sat, 15 Jan 2022 18:26:04: 5000000 INFO @ Sat, 15 Jan 2022 18:26:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:26:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:26:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:26:10: 12000000 INFO @ Sat, 15 Jan 2022 18:26:10: 6000000 INFO @ Sat, 15 Jan 2022 18:26:11: 1000000 INFO @ Sat, 15 Jan 2022 18:26:16: 13000000 INFO @ Sat, 15 Jan 2022 18:26:16: 7000000 INFO @ Sat, 15 Jan 2022 18:26:17: 2000000 INFO @ Sat, 15 Jan 2022 18:26:22: 14000000 INFO @ Sat, 15 Jan 2022 18:26:22: 8000000 INFO @ Sat, 15 Jan 2022 18:26:24: 3000000 INFO @ Sat, 15 Jan 2022 18:26:28: 15000000 INFO @ Sat, 15 Jan 2022 18:26:28: 9000000 INFO @ Sat, 15 Jan 2022 18:26:30: 4000000 INFO @ Sat, 15 Jan 2022 18:26:33: 16000000 INFO @ Sat, 15 Jan 2022 18:26:34: 10000000 INFO @ Sat, 15 Jan 2022 18:26:36: 5000000 INFO @ Sat, 15 Jan 2022 18:26:39: 17000000 INFO @ Sat, 15 Jan 2022 18:26:40: 11000000 INFO @ Sat, 15 Jan 2022 18:26:43: 6000000 INFO @ Sat, 15 Jan 2022 18:26:45: 18000000 INFO @ Sat, 15 Jan 2022 18:26:45: 12000000 INFO @ Sat, 15 Jan 2022 18:26:49: 7000000 INFO @ Sat, 15 Jan 2022 18:26:51: 19000000 INFO @ Sat, 15 Jan 2022 18:26:51: 13000000 INFO @ Sat, 15 Jan 2022 18:26:54: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:26:54: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:26:54: #1 total tags in treatment: 19456784 INFO @ Sat, 15 Jan 2022 18:26:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:26:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:26:54: #1 tags after filtering in treatment: 19456784 INFO @ Sat, 15 Jan 2022 18:26:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:26:54: #1 finished! INFO @ Sat, 15 Jan 2022 18:26:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:26:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:26:55: 8000000 INFO @ Sat, 15 Jan 2022 18:26:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:26:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:26:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:26:57: 14000000 INFO @ Sat, 15 Jan 2022 18:27:02: 9000000 INFO @ Sat, 15 Jan 2022 18:27:02: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:27:08: 16000000 INFO @ Sat, 15 Jan 2022 18:27:08: 10000000 INFO @ Sat, 15 Jan 2022 18:27:13: 17000000 INFO @ Sat, 15 Jan 2022 18:27:14: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:27:19: 18000000 INFO @ Sat, 15 Jan 2022 18:27:21: 12000000 INFO @ Sat, 15 Jan 2022 18:27:25: 19000000 INFO @ Sat, 15 Jan 2022 18:27:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:27:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:27:27: #1 total tags in treatment: 19456784 INFO @ Sat, 15 Jan 2022 18:27:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:27:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:27:27: 13000000 INFO @ Sat, 15 Jan 2022 18:27:28: #1 tags after filtering in treatment: 19456784 INFO @ Sat, 15 Jan 2022 18:27:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:27:28: #1 finished! INFO @ Sat, 15 Jan 2022 18:27:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:27:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:27:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:27:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:27:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:27:34: 14000000 INFO @ Sat, 15 Jan 2022 18:27:40: 15000000 INFO @ Sat, 15 Jan 2022 18:27:47: 16000000 INFO @ Sat, 15 Jan 2022 18:27:53: 17000000 INFO @ Sat, 15 Jan 2022 18:28:00: 18000000 INFO @ Sat, 15 Jan 2022 18:28:06: 19000000 INFO @ Sat, 15 Jan 2022 18:28:10: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:28:10: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:28:10: #1 total tags in treatment: 19456784 INFO @ Sat, 15 Jan 2022 18:28:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:28:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:28:10: #1 tags after filtering in treatment: 19456784 INFO @ Sat, 15 Jan 2022 18:28:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:28:10: #1 finished! INFO @ Sat, 15 Jan 2022 18:28:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:28:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:28:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:28:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:28:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101434/SRX10101434.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling