Job ID = 14519911 SRX = SRX10101433 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 19962241 spots for SRR13712695/SRR13712695.sra Written 19962241 spots for SRR13712695/SRR13712695.sra Read 11509133 spots for SRR13712696/SRR13712696.sra Written 11509133 spots for SRR13712696/SRR13712696.sra Read 6607606 spots for SRR13712697/SRR13712697.sra Written 6607606 spots for SRR13712697/SRR13712697.sra Read 21342474 spots for SRR13712698/SRR13712698.sra Written 21342474 spots for SRR13712698/SRR13712698.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:45 59421454 reads; of these: 59421454 (100.00%) were unpaired; of these: 4091027 (6.88%) aligned 0 times 45326452 (76.28%) aligned exactly 1 time 10003975 (16.84%) aligned >1 times 93.12% overall alignment rate Time searching: 00:12:45 Overall time: 00:12:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 35235245 / 55330427 = 0.6368 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:39:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:39:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:39:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:39:45: 1000000 INFO @ Sat, 15 Jan 2022 18:39:55: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:40:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:40:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:40:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:40:06: 3000000 INFO @ Sat, 15 Jan 2022 18:40:13: 1000000 INFO @ Sat, 15 Jan 2022 18:40:16: 4000000 INFO @ Sat, 15 Jan 2022 18:40:23: 2000000 INFO @ Sat, 15 Jan 2022 18:40:27: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:40:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:40:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:40:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:40:35: 3000000 INFO @ Sat, 15 Jan 2022 18:40:39: 6000000 INFO @ Sat, 15 Jan 2022 18:40:45: 1000000 INFO @ Sat, 15 Jan 2022 18:40:46: 4000000 INFO @ Sat, 15 Jan 2022 18:40:49: 7000000 INFO @ Sat, 15 Jan 2022 18:41:09: 2000000 INFO @ Sat, 15 Jan 2022 18:41:10: 5000000 INFO @ Sat, 15 Jan 2022 18:41:13: 8000000 INFO @ Sat, 15 Jan 2022 18:41:20: 6000000 INFO @ Sat, 15 Jan 2022 18:41:21: 3000000 INFO @ Sat, 15 Jan 2022 18:41:23: 9000000 INFO @ Sat, 15 Jan 2022 18:41:31: 7000000 INFO @ Sat, 15 Jan 2022 18:41:33: 4000000 INFO @ Sat, 15 Jan 2022 18:41:34: 10000000 INFO @ Sat, 15 Jan 2022 18:41:41: 8000000 INFO @ Sat, 15 Jan 2022 18:41:46: 11000000 INFO @ Sat, 15 Jan 2022 18:41:46: 5000000 INFO @ Sat, 15 Jan 2022 18:41:57: 9000000 INFO @ Sat, 15 Jan 2022 18:41:59: 12000000 INFO @ Sat, 15 Jan 2022 18:42:00: 6000000 INFO @ Sat, 15 Jan 2022 18:42:07: 10000000 INFO @ Sat, 15 Jan 2022 18:42:09: 13000000 INFO @ Sat, 15 Jan 2022 18:42:11: 7000000 INFO @ Sat, 15 Jan 2022 18:42:17: 11000000 INFO @ Sat, 15 Jan 2022 18:42:18: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:42:23: 8000000 INFO @ Sat, 15 Jan 2022 18:42:27: 12000000 INFO @ Sat, 15 Jan 2022 18:42:28: 15000000 INFO @ Sat, 15 Jan 2022 18:42:34: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:42:37: 13000000 INFO @ Sat, 15 Jan 2022 18:42:38: 16000000 INFO @ Sat, 15 Jan 2022 18:42:44: 10000000 INFO @ Sat, 15 Jan 2022 18:42:48: 14000000 INFO @ Sat, 15 Jan 2022 18:42:48: 17000000 INFO @ Sat, 15 Jan 2022 18:42:55: 11000000 INFO @ Sat, 15 Jan 2022 18:42:58: 15000000 INFO @ Sat, 15 Jan 2022 18:42:58: 18000000 INFO @ Sat, 15 Jan 2022 18:43:06: 12000000 INFO @ Sat, 15 Jan 2022 18:43:08: 19000000 INFO @ Sat, 15 Jan 2022 18:43:08: 16000000 INFO @ Sat, 15 Jan 2022 18:43:17: 13000000 INFO @ Sat, 15 Jan 2022 18:43:18: 20000000 INFO @ Sat, 15 Jan 2022 18:43:18: 17000000 INFO @ Sat, 15 Jan 2022 18:43:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:43:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:43:19: #1 total tags in treatment: 20095182 INFO @ Sat, 15 Jan 2022 18:43:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:43:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:43:19: #1 tags after filtering in treatment: 20095182 INFO @ Sat, 15 Jan 2022 18:43:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:43:19: #1 finished! INFO @ Sat, 15 Jan 2022 18:43:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:43:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:43:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:43:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:43:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:43:27: 14000000 INFO @ Sat, 15 Jan 2022 18:43:29: 18000000 INFO @ Sat, 15 Jan 2022 18:43:38: 15000000 INFO @ Sat, 15 Jan 2022 18:43:39: 19000000 INFO @ Sat, 15 Jan 2022 18:43:48: 16000000 INFO @ Sat, 15 Jan 2022 18:43:49: 20000000 INFO @ Sat, 15 Jan 2022 18:43:50: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:43:50: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:43:50: #1 total tags in treatment: 20095182 INFO @ Sat, 15 Jan 2022 18:43:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:43:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:43:51: #1 tags after filtering in treatment: 20095182 INFO @ Sat, 15 Jan 2022 18:43:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:43:51: #1 finished! INFO @ Sat, 15 Jan 2022 18:43:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:43:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:43:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:43:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:43:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:43:58: 17000000 INFO @ Sat, 15 Jan 2022 18:44:07: 18000000 INFO @ Sat, 15 Jan 2022 18:44:17: 19000000 INFO @ Sat, 15 Jan 2022 18:44:27: 20000000 INFO @ Sat, 15 Jan 2022 18:44:28: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:44:28: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:44:28: #1 total tags in treatment: 20095182 INFO @ Sat, 15 Jan 2022 18:44:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:44:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:44:29: #1 tags after filtering in treatment: 20095182 INFO @ Sat, 15 Jan 2022 18:44:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:44:29: #1 finished! INFO @ Sat, 15 Jan 2022 18:44:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:44:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:44:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:44:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:44:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101433/SRX10101433.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling