Job ID = 14519904 SRX = SRX10101432 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 9847765 spots for SRR13712691/SRR13712691.sra Written 9847765 spots for SRR13712691/SRR13712691.sra Read 19076744 spots for SRR13712692/SRR13712692.sra Written 19076744 spots for SRR13712692/SRR13712692.sra Read 9214048 spots for SRR13712693/SRR13712693.sra Written 9214048 spots for SRR13712693/SRR13712693.sra Read 12410133 spots for SRR13712694/SRR13712694.sra Written 12410133 spots for SRR13712694/SRR13712694.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:17 50548690 reads; of these: 50548690 (100.00%) were unpaired; of these: 3414875 (6.76%) aligned 0 times 38162767 (75.50%) aligned exactly 1 time 8971048 (17.75%) aligned >1 times 93.24% overall alignment rate Time searching: 00:10:17 Overall time: 00:10:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 28268668 / 47133815 = 0.5998 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:29:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:29:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:29:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:29:29: 1000000 INFO @ Sat, 15 Jan 2022 18:29:37: 2000000 INFO @ Sat, 15 Jan 2022 18:29:45: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:29:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:29:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:29:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:29:53: 4000000 INFO @ Sat, 15 Jan 2022 18:29:58: 1000000 INFO @ Sat, 15 Jan 2022 18:30:03: 5000000 INFO @ Sat, 15 Jan 2022 18:30:07: 2000000 INFO @ Sat, 15 Jan 2022 18:30:12: 6000000 INFO @ Sat, 15 Jan 2022 18:30:15: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:30:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:30:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:30:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:30:20: 7000000 INFO @ Sat, 15 Jan 2022 18:30:24: 4000000 INFO @ Sat, 15 Jan 2022 18:30:30: 1000000 INFO @ Sat, 15 Jan 2022 18:30:30: 8000000 INFO @ Sat, 15 Jan 2022 18:30:34: 5000000 INFO @ Sat, 15 Jan 2022 18:30:39: 2000000 INFO @ Sat, 15 Jan 2022 18:30:40: 9000000 INFO @ Sat, 15 Jan 2022 18:30:43: 6000000 INFO @ Sat, 15 Jan 2022 18:30:49: 3000000 INFO @ Sat, 15 Jan 2022 18:30:49: 10000000 INFO @ Sat, 15 Jan 2022 18:30:52: 7000000 INFO @ Sat, 15 Jan 2022 18:30:58: 4000000 INFO @ Sat, 15 Jan 2022 18:30:58: 11000000 INFO @ Sat, 15 Jan 2022 18:31:01: 8000000 INFO @ Sat, 15 Jan 2022 18:31:06: 5000000 INFO @ Sat, 15 Jan 2022 18:31:07: 12000000 INFO @ Sat, 15 Jan 2022 18:31:10: 9000000 INFO @ Sat, 15 Jan 2022 18:31:15: 6000000 INFO @ Sat, 15 Jan 2022 18:31:15: 13000000 INFO @ Sat, 15 Jan 2022 18:31:19: 10000000 INFO @ Sat, 15 Jan 2022 18:31:24: 14000000 INFO @ Sat, 15 Jan 2022 18:31:24: 7000000 INFO @ Sat, 15 Jan 2022 18:31:28: 11000000 INFO @ Sat, 15 Jan 2022 18:31:34: 15000000 INFO @ Sat, 15 Jan 2022 18:31:34: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:31:38: 12000000 INFO @ Sat, 15 Jan 2022 18:31:44: 9000000 INFO @ Sat, 15 Jan 2022 18:31:44: 16000000 INFO @ Sat, 15 Jan 2022 18:31:47: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:31:53: 10000000 INFO @ Sat, 15 Jan 2022 18:31:53: 17000000 INFO @ Sat, 15 Jan 2022 18:31:56: 14000000 INFO @ Sat, 15 Jan 2022 18:32:03: 11000000 INFO @ Sat, 15 Jan 2022 18:32:03: 18000000 INFO @ Sat, 15 Jan 2022 18:32:06: 15000000 INFO @ Sat, 15 Jan 2022 18:32:11: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:32:11: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:32:11: #1 total tags in treatment: 18865147 INFO @ Sat, 15 Jan 2022 18:32:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:32:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:32:12: #1 tags after filtering in treatment: 18865147 INFO @ Sat, 15 Jan 2022 18:32:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:32:12: #1 finished! INFO @ Sat, 15 Jan 2022 18:32:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:32:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:32:12: 12000000 INFO @ Sat, 15 Jan 2022 18:32:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:32:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:32:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:32:15: 16000000 INFO @ Sat, 15 Jan 2022 18:32:21: 13000000 INFO @ Sat, 15 Jan 2022 18:32:23: 17000000 INFO @ Sat, 15 Jan 2022 18:32:29: 14000000 INFO @ Sat, 15 Jan 2022 18:32:32: 18000000 INFO @ Sat, 15 Jan 2022 18:32:38: 15000000 INFO @ Sat, 15 Jan 2022 18:32:39: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:32:39: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:32:39: #1 total tags in treatment: 18865147 INFO @ Sat, 15 Jan 2022 18:32:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:32:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:32:40: #1 tags after filtering in treatment: 18865147 INFO @ Sat, 15 Jan 2022 18:32:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:32:40: #1 finished! INFO @ Sat, 15 Jan 2022 18:32:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:32:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:32:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:32:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:32:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:32:46: 16000000 INFO @ Sat, 15 Jan 2022 18:32:54: 17000000 INFO @ Sat, 15 Jan 2022 18:33:03: 18000000 INFO @ Sat, 15 Jan 2022 18:33:10: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:33:10: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:33:10: #1 total tags in treatment: 18865147 INFO @ Sat, 15 Jan 2022 18:33:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:33:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:33:10: #1 tags after filtering in treatment: 18865147 INFO @ Sat, 15 Jan 2022 18:33:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:33:10: #1 finished! INFO @ Sat, 15 Jan 2022 18:33:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:33:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:33:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:33:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:33:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101432/SRX10101432.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling