Job ID = 14519903 SRX = SRX10101431 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7584379 spots for SRR13712687/SRR13712687.sra Written 7584379 spots for SRR13712687/SRR13712687.sra Read 10969337 spots for SRR13712688/SRR13712688.sra Written 10969337 spots for SRR13712688/SRR13712688.sra Read 8249499 spots for SRR13712689/SRR13712689.sra Written 8249499 spots for SRR13712689/SRR13712689.sra Read 10623213 spots for SRR13712690/SRR13712690.sra Written 10623213 spots for SRR13712690/SRR13712690.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:16 37426428 reads; of these: 37426428 (100.00%) were unpaired; of these: 1664354 (4.45%) aligned 0 times 28868186 (77.13%) aligned exactly 1 time 6893888 (18.42%) aligned >1 times 95.55% overall alignment rate Time searching: 00:07:16 Overall time: 00:07:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 19187828 / 35762074 = 0.5365 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:19:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:19:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:19:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:19:30: 1000000 INFO @ Sat, 15 Jan 2022 18:19:38: 2000000 INFO @ Sat, 15 Jan 2022 18:19:47: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:19:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:19:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:19:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:19:55: 4000000 INFO @ Sat, 15 Jan 2022 18:19:59: 1000000 INFO @ Sat, 15 Jan 2022 18:20:03: 5000000 INFO @ Sat, 15 Jan 2022 18:20:08: 2000000 INFO @ Sat, 15 Jan 2022 18:20:12: 6000000 INFO @ Sat, 15 Jan 2022 18:20:17: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:20:21: 7000000 INFO @ Sat, 15 Jan 2022 18:20:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:20:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:20:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:20:25: 4000000 INFO @ Sat, 15 Jan 2022 18:20:29: 8000000 INFO @ Sat, 15 Jan 2022 18:20:29: 1000000 INFO @ Sat, 15 Jan 2022 18:20:34: 5000000 INFO @ Sat, 15 Jan 2022 18:20:37: 9000000 INFO @ Sat, 15 Jan 2022 18:20:38: 2000000 INFO @ Sat, 15 Jan 2022 18:20:42: 6000000 INFO @ Sat, 15 Jan 2022 18:20:46: 10000000 INFO @ Sat, 15 Jan 2022 18:20:46: 3000000 INFO @ Sat, 15 Jan 2022 18:20:51: 7000000 INFO @ Sat, 15 Jan 2022 18:20:54: 11000000 INFO @ Sat, 15 Jan 2022 18:20:55: 4000000 INFO @ Sat, 15 Jan 2022 18:20:59: 8000000 INFO @ Sat, 15 Jan 2022 18:21:03: 12000000 INFO @ Sat, 15 Jan 2022 18:21:04: 5000000 INFO @ Sat, 15 Jan 2022 18:21:08: 9000000 INFO @ Sat, 15 Jan 2022 18:21:11: 13000000 INFO @ Sat, 15 Jan 2022 18:21:12: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:21:16: 10000000 INFO @ Sat, 15 Jan 2022 18:21:20: 14000000 INFO @ Sat, 15 Jan 2022 18:21:21: 7000000 INFO @ Sat, 15 Jan 2022 18:21:25: 11000000 INFO @ Sat, 15 Jan 2022 18:21:28: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:21:30: 8000000 INFO @ Sat, 15 Jan 2022 18:21:33: 12000000 INFO @ Sat, 15 Jan 2022 18:21:37: 16000000 INFO @ Sat, 15 Jan 2022 18:21:38: 9000000 INFO @ Sat, 15 Jan 2022 18:21:41: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:21:41: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:21:41: #1 total tags in treatment: 16574246 INFO @ Sat, 15 Jan 2022 18:21:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:21:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:21:42: #1 tags after filtering in treatment: 16574246 INFO @ Sat, 15 Jan 2022 18:21:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:21:42: #1 finished! INFO @ Sat, 15 Jan 2022 18:21:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:21:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:21:42: 13000000 INFO @ Sat, 15 Jan 2022 18:21:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:21:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:21:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:21:47: 10000000 INFO @ Sat, 15 Jan 2022 18:21:50: 14000000 INFO @ Sat, 15 Jan 2022 18:21:55: 11000000 INFO @ Sat, 15 Jan 2022 18:21:59: 15000000 INFO @ Sat, 15 Jan 2022 18:22:04: 12000000 INFO @ Sat, 15 Jan 2022 18:22:07: 16000000 INFO @ Sat, 15 Jan 2022 18:22:12: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:22:12: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:22:12: #1 total tags in treatment: 16574246 INFO @ Sat, 15 Jan 2022 18:22:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:22:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:22:12: 13000000 INFO @ Sat, 15 Jan 2022 18:22:12: #1 tags after filtering in treatment: 16574246 INFO @ Sat, 15 Jan 2022 18:22:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:22:12: #1 finished! INFO @ Sat, 15 Jan 2022 18:22:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:22:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:22:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:22:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:22:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:22:20: 14000000 INFO @ Sat, 15 Jan 2022 18:22:29: 15000000 INFO @ Sat, 15 Jan 2022 18:22:37: 16000000 INFO @ Sat, 15 Jan 2022 18:22:42: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:22:42: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:22:42: #1 total tags in treatment: 16574246 INFO @ Sat, 15 Jan 2022 18:22:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:22:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:22:42: #1 tags after filtering in treatment: 16574246 INFO @ Sat, 15 Jan 2022 18:22:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:22:42: #1 finished! INFO @ Sat, 15 Jan 2022 18:22:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:22:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:22:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:22:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:22:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101431/SRX10101431.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling