Job ID = 14519858 SRX = SRX10101429 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7964809 spots for SRR13712679/SRR13712679.sra Written 7964809 spots for SRR13712679/SRR13712679.sra Read 7487858 spots for SRR13712680/SRR13712680.sra Written 7487858 spots for SRR13712680/SRR13712680.sra Read 9413151 spots for SRR13712681/SRR13712681.sra Written 9413151 spots for SRR13712681/SRR13712681.sra Read 9727867 spots for SRR13712682/SRR13712682.sra Written 9727867 spots for SRR13712682/SRR13712682.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:48 34593685 reads; of these: 34593685 (100.00%) were unpaired; of these: 3563658 (10.30%) aligned 0 times 25319000 (73.19%) aligned exactly 1 time 5711027 (16.51%) aligned >1 times 89.70% overall alignment rate Time searching: 00:04:48 Overall time: 00:04:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 16849466 / 31030027 = 0.5430 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:08:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:08:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:08:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:08:14: 1000000 INFO @ Sat, 15 Jan 2022 18:08:19: 2000000 INFO @ Sat, 15 Jan 2022 18:08:24: 3000000 INFO @ Sat, 15 Jan 2022 18:08:29: 4000000 INFO @ Sat, 15 Jan 2022 18:08:34: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:08:38: 6000000 INFO @ Sat, 15 Jan 2022 18:08:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:08:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:08:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:08:44: 7000000 INFO @ Sat, 15 Jan 2022 18:08:45: 1000000 INFO @ Sat, 15 Jan 2022 18:08:49: 8000000 INFO @ Sat, 15 Jan 2022 18:08:50: 2000000 INFO @ Sat, 15 Jan 2022 18:08:55: 9000000 INFO @ Sat, 15 Jan 2022 18:08:55: 3000000 INFO @ Sat, 15 Jan 2022 18:09:00: 10000000 INFO @ Sat, 15 Jan 2022 18:09:01: 4000000 INFO @ Sat, 15 Jan 2022 18:09:05: 11000000 INFO @ Sat, 15 Jan 2022 18:09:06: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:09:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:09:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:09:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:09:11: 12000000 INFO @ Sat, 15 Jan 2022 18:09:12: 6000000 INFO @ Sat, 15 Jan 2022 18:09:15: 1000000 INFO @ Sat, 15 Jan 2022 18:09:17: 13000000 INFO @ Sat, 15 Jan 2022 18:09:17: 7000000 INFO @ Sat, 15 Jan 2022 18:09:21: 2000000 INFO @ Sat, 15 Jan 2022 18:09:23: 14000000 INFO @ Sat, 15 Jan 2022 18:09:23: 8000000 INFO @ Sat, 15 Jan 2022 18:09:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:09:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:09:24: #1 total tags in treatment: 14180561 INFO @ Sat, 15 Jan 2022 18:09:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:09:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:09:24: #1 tags after filtering in treatment: 14180561 INFO @ Sat, 15 Jan 2022 18:09:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:09:24: #1 finished! INFO @ Sat, 15 Jan 2022 18:09:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:09:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:09:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:09:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:09:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:09:27: 3000000 INFO @ Sat, 15 Jan 2022 18:09:28: 9000000 INFO @ Sat, 15 Jan 2022 18:09:32: 4000000 INFO @ Sat, 15 Jan 2022 18:09:34: 10000000 INFO @ Sat, 15 Jan 2022 18:09:37: 5000000 INFO @ Sat, 15 Jan 2022 18:09:39: 11000000 INFO @ Sat, 15 Jan 2022 18:09:43: 6000000 INFO @ Sat, 15 Jan 2022 18:09:44: 12000000 INFO @ Sat, 15 Jan 2022 18:09:48: 7000000 INFO @ Sat, 15 Jan 2022 18:09:50: 13000000 INFO @ Sat, 15 Jan 2022 18:09:54: 8000000 INFO @ Sat, 15 Jan 2022 18:09:55: 14000000 INFO @ Sat, 15 Jan 2022 18:09:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:09:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:09:56: #1 total tags in treatment: 14180561 INFO @ Sat, 15 Jan 2022 18:09:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:09:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:09:56: #1 tags after filtering in treatment: 14180561 INFO @ Sat, 15 Jan 2022 18:09:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:09:56: #1 finished! INFO @ Sat, 15 Jan 2022 18:09:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:09:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:09:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:09:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:09:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:09:59: 9000000 INFO @ Sat, 15 Jan 2022 18:10:03: 10000000 INFO @ Sat, 15 Jan 2022 18:10:08: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:10:13: 12000000 INFO @ Sat, 15 Jan 2022 18:10:18: 13000000 INFO @ Sat, 15 Jan 2022 18:10:23: 14000000 INFO @ Sat, 15 Jan 2022 18:10:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:10:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:10:24: #1 total tags in treatment: 14180561 INFO @ Sat, 15 Jan 2022 18:10:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:10:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:10:24: #1 tags after filtering in treatment: 14180561 INFO @ Sat, 15 Jan 2022 18:10:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:10:24: #1 finished! INFO @ Sat, 15 Jan 2022 18:10:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:10:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:10:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:10:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:10:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101429/SRX10101429.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling