Job ID = 14519854 SRX = SRX10101427 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 9251838 spots for SRR13712671/SRR13712671.sra Written 9251838 spots for SRR13712671/SRR13712671.sra Read 9779847 spots for SRR13712672/SRR13712672.sra Written 9779847 spots for SRR13712672/SRR13712672.sra Read 6753016 spots for SRR13712673/SRR13712673.sra Written 6753016 spots for SRR13712673/SRR13712673.sra Read 7880493 spots for SRR13712674/SRR13712674.sra Written 7880493 spots for SRR13712674/SRR13712674.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:36 33665194 reads; of these: 33665194 (100.00%) were unpaired; of these: 3556827 (10.57%) aligned 0 times 25043470 (74.39%) aligned exactly 1 time 5064897 (15.04%) aligned >1 times 89.43% overall alignment rate Time searching: 00:05:36 Overall time: 00:05:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15249164 / 30108367 = 0.5065 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:09:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:09:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:09:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:09:16: 1000000 INFO @ Sat, 15 Jan 2022 18:09:22: 2000000 INFO @ Sat, 15 Jan 2022 18:09:27: 3000000 INFO @ Sat, 15 Jan 2022 18:09:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:09:39: 5000000 INFO @ Sat, 15 Jan 2022 18:09:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:09:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:09:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:09:45: 6000000 INFO @ Sat, 15 Jan 2022 18:09:46: 1000000 INFO @ Sat, 15 Jan 2022 18:09:51: 7000000 INFO @ Sat, 15 Jan 2022 18:09:53: 2000000 INFO @ Sat, 15 Jan 2022 18:09:58: 8000000 INFO @ Sat, 15 Jan 2022 18:09:59: 3000000 INFO @ Sat, 15 Jan 2022 18:10:04: 9000000 INFO @ Sat, 15 Jan 2022 18:10:05: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:10:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:10:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:10:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:10:10: 10000000 INFO @ Sat, 15 Jan 2022 18:10:11: 5000000 INFO @ Sat, 15 Jan 2022 18:10:17: 1000000 INFO @ Sat, 15 Jan 2022 18:10:17: 11000000 INFO @ Sat, 15 Jan 2022 18:10:18: 6000000 INFO @ Sat, 15 Jan 2022 18:10:23: 2000000 INFO @ Sat, 15 Jan 2022 18:10:24: 12000000 INFO @ Sat, 15 Jan 2022 18:10:25: 7000000 INFO @ Sat, 15 Jan 2022 18:10:30: 3000000 INFO @ Sat, 15 Jan 2022 18:10:30: 13000000 INFO @ Sat, 15 Jan 2022 18:10:31: 8000000 INFO @ Sat, 15 Jan 2022 18:10:36: 4000000 INFO @ Sat, 15 Jan 2022 18:10:37: 9000000 INFO @ Sat, 15 Jan 2022 18:10:37: 14000000 INFO @ Sat, 15 Jan 2022 18:10:43: 5000000 INFO @ Sat, 15 Jan 2022 18:10:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:10:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:10:43: #1 total tags in treatment: 14859203 INFO @ Sat, 15 Jan 2022 18:10:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:10:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:10:43: #1 tags after filtering in treatment: 14859203 INFO @ Sat, 15 Jan 2022 18:10:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:10:43: #1 finished! INFO @ Sat, 15 Jan 2022 18:10:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:10:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:10:44: 10000000 INFO @ Sat, 15 Jan 2022 18:10:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:10:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:10:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:10:49: 6000000 INFO @ Sat, 15 Jan 2022 18:10:49: 11000000 INFO @ Sat, 15 Jan 2022 18:10:55: 12000000 INFO @ Sat, 15 Jan 2022 18:10:56: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:11:01: 13000000 INFO @ Sat, 15 Jan 2022 18:11:02: 8000000 INFO @ Sat, 15 Jan 2022 18:11:07: 14000000 INFO @ Sat, 15 Jan 2022 18:11:09: 9000000 INFO @ Sat, 15 Jan 2022 18:11:13: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:11:13: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:11:13: #1 total tags in treatment: 14859203 INFO @ Sat, 15 Jan 2022 18:11:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:11:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:11:13: #1 tags after filtering in treatment: 14859203 INFO @ Sat, 15 Jan 2022 18:11:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:11:13: #1 finished! INFO @ Sat, 15 Jan 2022 18:11:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:11:13: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:11:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:11:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:11:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:11:15: 10000000 INFO @ Sat, 15 Jan 2022 18:11:22: 11000000 INFO @ Sat, 15 Jan 2022 18:11:28: 12000000 INFO @ Sat, 15 Jan 2022 18:11:35: 13000000 INFO @ Sat, 15 Jan 2022 18:11:41: 14000000 INFO @ Sat, 15 Jan 2022 18:11:47: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:11:47: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:11:47: #1 total tags in treatment: 14859203 INFO @ Sat, 15 Jan 2022 18:11:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:11:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:11:47: #1 tags after filtering in treatment: 14859203 INFO @ Sat, 15 Jan 2022 18:11:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:11:47: #1 finished! INFO @ Sat, 15 Jan 2022 18:11:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:11:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:11:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:11:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:11:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101427/SRX10101427.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling