Job ID = 14519849 SRX = SRX10101424 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5816563 spots for SRR13712659/SRR13712659.sra Written 5816563 spots for SRR13712659/SRR13712659.sra Read 8077546 spots for SRR13712660/SRR13712660.sra Written 8077546 spots for SRR13712660/SRR13712660.sra Read 3191270 spots for SRR13712661/SRR13712661.sra Written 3191270 spots for SRR13712661/SRR13712661.sra Read 3926096 spots for SRR13712662/SRR13712662.sra Written 3926096 spots for SRR13712662/SRR13712662.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:49 21011475 reads; of these: 21011475 (100.00%) were unpaired; of these: 2363831 (11.25%) aligned 0 times 15694529 (74.70%) aligned exactly 1 time 2953115 (14.05%) aligned >1 times 88.75% overall alignment rate Time searching: 00:02:50 Overall time: 00:02:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10559250 / 18647644 = 0.5663 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:00:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:00:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:00:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:00:52: 1000000 INFO @ Sat, 15 Jan 2022 18:00:58: 2000000 INFO @ Sat, 15 Jan 2022 18:01:03: 3000000 INFO @ Sat, 15 Jan 2022 18:01:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:01:15: 5000000 INFO @ Sat, 15 Jan 2022 18:01:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:01:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:01:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:01:21: 6000000 INFO @ Sat, 15 Jan 2022 18:01:22: 1000000 INFO @ Sat, 15 Jan 2022 18:01:28: 7000000 INFO @ Sat, 15 Jan 2022 18:01:29: 2000000 INFO @ Sat, 15 Jan 2022 18:01:34: 8000000 INFO @ Sat, 15 Jan 2022 18:01:35: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:01:35: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:01:35: #1 total tags in treatment: 8088394 INFO @ Sat, 15 Jan 2022 18:01:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:01:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:01:35: #1 tags after filtering in treatment: 8088394 INFO @ Sat, 15 Jan 2022 18:01:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:01:35: #1 finished! INFO @ Sat, 15 Jan 2022 18:01:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:01:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:01:35: 3000000 INFO @ Sat, 15 Jan 2022 18:01:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:01:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:01:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:01:41: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:01:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:01:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:01:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:01:47: 5000000 INFO @ Sat, 15 Jan 2022 18:01:52: 1000000 INFO @ Sat, 15 Jan 2022 18:01:54: 6000000 INFO @ Sat, 15 Jan 2022 18:01:59: 2000000 INFO @ Sat, 15 Jan 2022 18:02:00: 7000000 INFO @ Sat, 15 Jan 2022 18:02:05: 3000000 INFO @ Sat, 15 Jan 2022 18:02:07: 8000000 INFO @ Sat, 15 Jan 2022 18:02:07: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:02:07: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:02:07: #1 total tags in treatment: 8088394 INFO @ Sat, 15 Jan 2022 18:02:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:02:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:02:07: #1 tags after filtering in treatment: 8088394 INFO @ Sat, 15 Jan 2022 18:02:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:02:07: #1 finished! INFO @ Sat, 15 Jan 2022 18:02:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:02:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:02:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:02:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:02:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:02:12: 4000000 INFO @ Sat, 15 Jan 2022 18:02:17: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:02:23: 6000000 INFO @ Sat, 15 Jan 2022 18:02:28: 7000000 INFO @ Sat, 15 Jan 2022 18:02:34: 8000000 INFO @ Sat, 15 Jan 2022 18:02:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:02:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:02:34: #1 total tags in treatment: 8088394 INFO @ Sat, 15 Jan 2022 18:02:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:02:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:02:34: #1 tags after filtering in treatment: 8088394 INFO @ Sat, 15 Jan 2022 18:02:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:02:34: #1 finished! INFO @ Sat, 15 Jan 2022 18:02:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:02:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:02:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:02:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:02:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101424/SRX10101424.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling