Job ID = 14519848 SRX = SRX10101423 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7225961 spots for SRR13712655/SRR13712655.sra Written 7225961 spots for SRR13712655/SRR13712655.sra Read 6148091 spots for SRR13712656/SRR13712656.sra Written 6148091 spots for SRR13712656/SRR13712656.sra Read 2342636 spots for SRR13712657/SRR13712657.sra Written 2342636 spots for SRR13712657/SRR13712657.sra Read 2620150 spots for SRR13712658/SRR13712658.sra Written 2620150 spots for SRR13712658/SRR13712658.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:07 18336838 reads; of these: 18336838 (100.00%) were unpaired; of these: 2334897 (12.73%) aligned 0 times 13253598 (72.28%) aligned exactly 1 time 2748343 (14.99%) aligned >1 times 87.27% overall alignment rate Time searching: 00:04:07 Overall time: 00:04:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8258789 / 16001941 = 0.5161 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:02:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:02:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:02:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:02:18: 1000000 INFO @ Sat, 15 Jan 2022 18:02:27: 2000000 INFO @ Sat, 15 Jan 2022 18:02:34: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:02:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:02:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:02:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:02:42: 4000000 INFO @ Sat, 15 Jan 2022 18:02:50: 1000000 INFO @ Sat, 15 Jan 2022 18:02:50: 5000000 INFO @ Sat, 15 Jan 2022 18:02:58: 6000000 INFO @ Sat, 15 Jan 2022 18:03:00: 2000000 INFO @ Sat, 15 Jan 2022 18:03:07: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:03:10: 3000000 INFO @ Sat, 15 Jan 2022 18:03:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:03:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:03:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:03:13: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:03:13: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:03:13: #1 total tags in treatment: 7743152 INFO @ Sat, 15 Jan 2022 18:03:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:03:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:03:13: #1 tags after filtering in treatment: 7743152 INFO @ Sat, 15 Jan 2022 18:03:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:03:13: #1 finished! INFO @ Sat, 15 Jan 2022 18:03:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:03:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:03:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:03:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:03:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:03:21: 4000000 INFO @ Sat, 15 Jan 2022 18:03:21: 1000000 INFO @ Sat, 15 Jan 2022 18:03:31: 5000000 INFO @ Sat, 15 Jan 2022 18:03:31: 2000000 INFO @ Sat, 15 Jan 2022 18:03:42: 6000000 INFO @ Sat, 15 Jan 2022 18:03:42: 3000000 INFO @ Sat, 15 Jan 2022 18:03:52: 7000000 INFO @ Sat, 15 Jan 2022 18:03:52: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:04:00: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:04:00: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:04:00: #1 total tags in treatment: 7743152 INFO @ Sat, 15 Jan 2022 18:04:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:04:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:04:00: #1 tags after filtering in treatment: 7743152 INFO @ Sat, 15 Jan 2022 18:04:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:04:00: #1 finished! INFO @ Sat, 15 Jan 2022 18:04:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:04:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:04:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:04:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:04:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:04:03: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:04:12: 6000000 INFO @ Sat, 15 Jan 2022 18:04:22: 7000000 INFO @ Sat, 15 Jan 2022 18:04:29: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:04:29: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:04:29: #1 total tags in treatment: 7743152 INFO @ Sat, 15 Jan 2022 18:04:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:04:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:04:29: #1 tags after filtering in treatment: 7743152 INFO @ Sat, 15 Jan 2022 18:04:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:04:29: #1 finished! INFO @ Sat, 15 Jan 2022 18:04:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:04:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:04:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:04:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:04:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10101423/SRX10101423.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling