Job ID = 2009663 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,323,652 reads read : 3,323,652 reads written : 3,323,652 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:29 3323652 reads; of these: 3323652 (100.00%) were unpaired; of these: 147591 (4.44%) aligned 0 times 2604948 (78.38%) aligned exactly 1 time 571113 (17.18%) aligned >1 times 95.56% overall alignment rate Time searching: 00:00:29 Overall time: 00:00:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 959783 / 3176061 = 0.3022 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:28:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:28:59: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:28:59: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:29:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:29:00: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:29:00: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:29:06: 1000000 INFO @ Fri, 05 Jul 2019 19:29:10: 1000000 INFO @ Fri, 05 Jul 2019 19:29:12: 2000000 INFO @ Fri, 05 Jul 2019 19:29:14: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:29:14: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:29:14: #1 total tags in treatment: 2216278 INFO @ Fri, 05 Jul 2019 19:29:14: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:29:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:29:14: #1 tags after filtering in treatment: 2216278 INFO @ Fri, 05 Jul 2019 19:29:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:29:14: #1 finished! INFO @ Fri, 05 Jul 2019 19:29:14: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:29:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:29:14: #2 number of paired peaks: 25 WARNING @ Fri, 05 Jul 2019 19:29:14: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:29:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:29:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:29:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:29:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:29:20: 2000000 INFO @ Fri, 05 Jul 2019 19:29:21: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:29:21: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:29:21: #1 total tags in treatment: 2216278 INFO @ Fri, 05 Jul 2019 19:29:21: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:29:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:29:21: #1 tags after filtering in treatment: 2216278 INFO @ Fri, 05 Jul 2019 19:29:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:29:21: #1 finished! INFO @ Fri, 05 Jul 2019 19:29:21: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:29:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:29:22: #2 number of paired peaks: 25 WARNING @ Fri, 05 Jul 2019 19:29:22: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:29:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:29:25: 1000000 INFO @ Fri, 05 Jul 2019 19:29:33: 2000000 INFO @ Fri, 05 Jul 2019 19:29:35: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:29:35: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:29:35: #1 total tags in treatment: 2216278 INFO @ Fri, 05 Jul 2019 19:29:35: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:29:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:29:35: #1 tags after filtering in treatment: 2216278 INFO @ Fri, 05 Jul 2019 19:29:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:29:35: #1 finished! INFO @ Fri, 05 Jul 2019 19:29:35: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:29:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:29:35: #2 number of paired peaks: 25 WARNING @ Fri, 05 Jul 2019 19:29:35: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:29:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100286/SRX100286.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。