Job ID = 2009658 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,701,212 reads read : 3,701,212 reads written : 3,701,212 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:35 3701212 reads; of these: 3701212 (100.00%) were unpaired; of these: 203199 (5.49%) aligned 0 times 3000169 (81.06%) aligned exactly 1 time 497844 (13.45%) aligned >1 times 94.51% overall alignment rate Time searching: 00:00:35 Overall time: 00:00:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 902465 / 3498013 = 0.2580 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:25:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:25:42: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:25:42: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:25:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:25:43: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:25:43: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:25:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:25:46: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:25:46: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:25:50: 1000000 INFO @ Fri, 05 Jul 2019 19:25:52: 1000000 INFO @ Fri, 05 Jul 2019 19:25:54: 1000000 INFO @ Fri, 05 Jul 2019 19:25:58: 2000000 INFO @ Fri, 05 Jul 2019 19:25:59: 2000000 INFO @ Fri, 05 Jul 2019 19:26:03: 2000000 INFO @ Fri, 05 Jul 2019 19:26:03: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:26:03: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:26:03: #1 total tags in treatment: 2595548 INFO @ Fri, 05 Jul 2019 19:26:03: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:26:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:26:03: #1 tags after filtering in treatment: 2595548 INFO @ Fri, 05 Jul 2019 19:26:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:26:03: #1 finished! INFO @ Fri, 05 Jul 2019 19:26:03: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:26:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:26:03: #2 number of paired peaks: 29 WARNING @ Fri, 05 Jul 2019 19:26:03: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:26:03: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:26:04: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:26:04: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:26:04: #1 total tags in treatment: 2595548 INFO @ Fri, 05 Jul 2019 19:26:04: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:26:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:26:04: #1 tags after filtering in treatment: 2595548 INFO @ Fri, 05 Jul 2019 19:26:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:26:04: #1 finished! INFO @ Fri, 05 Jul 2019 19:26:04: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:26:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:26:04: #2 number of paired peaks: 29 WARNING @ Fri, 05 Jul 2019 19:26:04: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:26:04: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:26:08: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:26:08: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:26:08: #1 total tags in treatment: 2595548 INFO @ Fri, 05 Jul 2019 19:26:08: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:26:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:26:08: #1 tags after filtering in treatment: 2595548 INFO @ Fri, 05 Jul 2019 19:26:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:26:08: #1 finished! INFO @ Fri, 05 Jul 2019 19:26:08: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:26:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:26:08: #2 number of paired peaks: 29 WARNING @ Fri, 05 Jul 2019 19:26:08: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:26:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.10_*.xls’: No such file or directory rm: rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.20_peaks.narrowPeak’cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.05_model.r’: No such file or directory : No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.10_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.05_*.xls’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100282/SRX100282.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。