Job ID = 2009657 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,202,068 reads read : 11,202,068 reads written : 11,202,068 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:35 11202068 reads; of these: 11202068 (100.00%) were unpaired; of these: 468794 (4.18%) aligned 0 times 9678450 (86.40%) aligned exactly 1 time 1054824 (9.42%) aligned >1 times 95.82% overall alignment rate Time searching: 00:01:35 Overall time: 00:01:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 4708660 / 10733274 = 0.4387 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:37:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:37:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:37:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:37:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:37:18: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:37:18: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:37:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:37:19: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:37:19: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:37:24: 1000000 INFO @ Fri, 05 Jul 2019 19:37:26: 1000000 INFO @ Fri, 05 Jul 2019 19:37:27: 1000000 INFO @ Fri, 05 Jul 2019 19:37:31: 2000000 INFO @ Fri, 05 Jul 2019 19:37:34: 2000000 INFO @ Fri, 05 Jul 2019 19:37:35: 2000000 INFO @ Fri, 05 Jul 2019 19:37:38: 3000000 INFO @ Fri, 05 Jul 2019 19:37:42: 3000000 INFO @ Fri, 05 Jul 2019 19:37:43: 3000000 INFO @ Fri, 05 Jul 2019 19:37:45: 4000000 INFO @ Fri, 05 Jul 2019 19:37:49: 4000000 INFO @ Fri, 05 Jul 2019 19:37:50: 4000000 INFO @ Fri, 05 Jul 2019 19:37:52: 5000000 INFO @ Fri, 05 Jul 2019 19:37:57: 5000000 INFO @ Fri, 05 Jul 2019 19:37:58: 5000000 INFO @ Fri, 05 Jul 2019 19:37:59: 6000000 INFO @ Fri, 05 Jul 2019 19:37:59: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:37:59: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:37:59: #1 total tags in treatment: 6024614 INFO @ Fri, 05 Jul 2019 19:37:59: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:59: #1 tags after filtering in treatment: 6024614 INFO @ Fri, 05 Jul 2019 19:37:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:59: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:59: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:38:00: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:38:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:38:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:38:04: 6000000 INFO @ Fri, 05 Jul 2019 19:38:04: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:38:04: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:38:04: #1 total tags in treatment: 6024614 INFO @ Fri, 05 Jul 2019 19:38:04: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:38:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:38:05: #1 tags after filtering in treatment: 6024614 INFO @ Fri, 05 Jul 2019 19:38:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:38:05: #1 finished! INFO @ Fri, 05 Jul 2019 19:38:05: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:38:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:38:05: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:38:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:38:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 41 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:38:06: 6000000 INFO @ Fri, 05 Jul 2019 19:38:06: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:38:06: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:38:06: #1 total tags in treatment: 6024614 INFO @ Fri, 05 Jul 2019 19:38:06: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:38:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:38:06: #1 tags after filtering in treatment: 6024614 INFO @ Fri, 05 Jul 2019 19:38:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:38:06: #1 finished! INFO @ Fri, 05 Jul 2019 19:38:06: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:38:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:38:06: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:38:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:38:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100281/SRX100281.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。