Job ID = 2009656 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 1,649,666 reads read : 1,649,666 reads written : 1,649,666 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:34 1649666 reads; of these: 1649666 (100.00%) were unpaired; of these: 112364 (6.81%) aligned 0 times 1257883 (76.25%) aligned exactly 1 time 279419 (16.94%) aligned >1 times 93.19% overall alignment rate Time searching: 00:00:34 Overall time: 00:00:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 369961 / 1537302 = 0.2407 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:19:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:19:41: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:19:41: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:19:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:19:43: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:19:43: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:19:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:19:43: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:19:43: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:19:51: 1000000 INFO @ Fri, 05 Jul 2019 19:19:52: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:19:52: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:19:52: #1 total tags in treatment: 1167341 INFO @ Fri, 05 Jul 2019 19:19:52: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:19:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:19:52: #1 tags after filtering in treatment: 1167341 INFO @ Fri, 05 Jul 2019 19:19:52: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:19:52: #1 finished! INFO @ Fri, 05 Jul 2019 19:19:52: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:19:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:19:52: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 19:19:52: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:19:52: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:19:52: 1000000 INFO @ Fri, 05 Jul 2019 19:19:52: 1000000 INFO @ Fri, 05 Jul 2019 19:19:53: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:19:53: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:19:53: #1 total tags in treatment: 1167341 INFO @ Fri, 05 Jul 2019 19:19:53: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:19:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:19:53: #1 tags after filtering in treatment: 1167341 INFO @ Fri, 05 Jul 2019 19:19:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:19:53: #1 finished! INFO @ Fri, 05 Jul 2019 19:19:53: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:19:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:19:53: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 19:19:53: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:19:53: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:19:54: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 19:19:54: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 19:19:54: #1 total tags in treatment: 1167341 INFO @ Fri, 05 Jul 2019 19:19:54: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:19:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:19:54: #1 tags after filtering in treatment: 1167341 INFO @ Fri, 05 Jul 2019 19:19:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:19:54: #1 finished! INFO @ Fri, 05 Jul 2019 19:19:54: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:19:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:19:54: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 19:19:54: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:19:54: Process for pairing-model is terminated! BedGraph に変換しました。 cut: /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.20_peaks.narrowPeak: No such file or directory BigWig に変換中... pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX100280/SRX100280.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling