Job ID = 9161702 sra ファイルのダウンロード中... Completed: 133674K bytes transferred in 4 seconds (223268K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 3788281 spots for /home/okishinya/chipatlas/results/sacCer3/SRX092427/SRR332213.sra Written 3788281 spots total Written 3884358 spots for /home/okishinya/chipatlas/results/sacCer3/SRX092427/SRR332214.sra Written 3884358 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:09 7672639 reads; of these: 7672639 (100.00%) were unpaired; of these: 914927 (11.92%) aligned 0 times 5078146 (66.19%) aligned exactly 1 time 1679566 (21.89%) aligned >1 times 88.08% overall alignment rate Time searching: 00:01:09 Overall time: 00:01:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2292430 / 6757712 = 0.3392 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:22:55: # Command line: callpeak -t SRX092427.bam -f BAM -g 12100000 -n SRX092427.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX092427.20 # format = BAM # ChIP-seq file = ['SRX092427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:22:55: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:22:55: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:22:55: # Command line: callpeak -t SRX092427.bam -f BAM -g 12100000 -n SRX092427.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX092427.10 # format = BAM # ChIP-seq file = ['SRX092427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:22:55: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:22:55: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:22:55: # Command line: callpeak -t SRX092427.bam -f BAM -g 12100000 -n SRX092427.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX092427.05 # format = BAM # ChIP-seq file = ['SRX092427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:22:55: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:22:55: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:23:02: 1000000 INFO @ Wed, 28 Jun 2017 04:23:02: 1000000 INFO @ Wed, 28 Jun 2017 04:23:02: 1000000 INFO @ Wed, 28 Jun 2017 04:23:08: 2000000 INFO @ Wed, 28 Jun 2017 04:23:09: 2000000 INFO @ Wed, 28 Jun 2017 04:23:09: 2000000 INFO @ Wed, 28 Jun 2017 04:23:15: 3000000 INFO @ Wed, 28 Jun 2017 04:23:16: 3000000 INFO @ Wed, 28 Jun 2017 04:23:16: 3000000 INFO @ Wed, 28 Jun 2017 04:23:22: 4000000 INFO @ Wed, 28 Jun 2017 04:23:24: 4000000 INFO @ Wed, 28 Jun 2017 04:23:24: 4000000 INFO @ Wed, 28 Jun 2017 04:23:25: #1 tag size is determined as 32 bps INFO @ Wed, 28 Jun 2017 04:23:25: #1 tag size = 32 INFO @ Wed, 28 Jun 2017 04:23:25: #1 total tags in treatment: 4465282 INFO @ Wed, 28 Jun 2017 04:23:25: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:23:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:23:25: #1 tags after filtering in treatment: 4465282 INFO @ Wed, 28 Jun 2017 04:23:25: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:23:25: #1 finished! INFO @ Wed, 28 Jun 2017 04:23:25: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:23:25: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:23:25: #2 number of paired peaks: 29 WARNING @ Wed, 28 Jun 2017 04:23:25: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:23:25: Process for pairing-model is terminated! cat: SRX092427.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX092427.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX092427.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX092427.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:23:27: #1 tag size is determined as 32 bps INFO @ Wed, 28 Jun 2017 04:23:27: #1 tag size = 32 INFO @ Wed, 28 Jun 2017 04:23:27: #1 total tags in treatment: 4465282 INFO @ Wed, 28 Jun 2017 04:23:27: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:23:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:23:27: #1 tag size is determined as 32 bps INFO @ Wed, 28 Jun 2017 04:23:27: #1 tag size = 32 INFO @ Wed, 28 Jun 2017 04:23:27: #1 total tags in treatment: 4465282 INFO @ Wed, 28 Jun 2017 04:23:27: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:23:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:23:27: #1 tags after filtering in treatment: 4465282 INFO @ Wed, 28 Jun 2017 04:23:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:23:27: #1 finished! INFO @ Wed, 28 Jun 2017 04:23:27: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:23:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:23:27: #1 tags after filtering in treatment: 4465282 INFO @ Wed, 28 Jun 2017 04:23:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:23:27: #1 finished! INFO @ Wed, 28 Jun 2017 04:23:27: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:23:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:23:27: #2 number of paired peaks: 29 WARNING @ Wed, 28 Jun 2017 04:23:27: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:23:27: Process for pairing-model is terminated! cat: SRX092427.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Wed, 28 Jun 2017 04:23:27: #2 number of paired peaks: 29 WARNING @ Wed, 28 Jun 2017 04:23:27: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:23:27: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX092427.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX092427.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX092427.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX092427.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX092427.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX092427.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX092427.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。