Job ID = 9161700 sra ファイルのダウンロード中... Completed: 46624K bytes transferred in 3 seconds (104963K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 1220867 spots for /home/okishinya/chipatlas/results/sacCer3/SRX092425/SRR332210.sra Written 1220867 spots total Written 1953477 spots for /home/okishinya/chipatlas/results/sacCer3/SRX092425/SRR332209.sra Written 1953477 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:20 3174344 reads; of these: 3174344 (100.00%) were unpaired; of these: 883669 (27.84%) aligned 0 times 1678364 (52.87%) aligned exactly 1 time 612311 (19.29%) aligned >1 times 72.16% overall alignment rate Time searching: 00:00:20 Overall time: 00:00:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 589617 / 2290675 = 0.2574 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:20:49: # Command line: callpeak -t SRX092425.bam -f BAM -g 12100000 -n SRX092425.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX092425.05 # format = BAM # ChIP-seq file = ['SRX092425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:20:49: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:20:49: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:20:49: # Command line: callpeak -t SRX092425.bam -f BAM -g 12100000 -n SRX092425.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX092425.20 # format = BAM # ChIP-seq file = ['SRX092425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:20:49: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:20:49: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:20:49: # Command line: callpeak -t SRX092425.bam -f BAM -g 12100000 -n SRX092425.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX092425.10 # format = BAM # ChIP-seq file = ['SRX092425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:20:49: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:20:49: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:20:55: 1000000 INFO @ Wed, 28 Jun 2017 04:20:55: 1000000 INFO @ Wed, 28 Jun 2017 04:20:55: 1000000 INFO @ Wed, 28 Jun 2017 04:20:59: #1 tag size is determined as 32 bps INFO @ Wed, 28 Jun 2017 04:20:59: #1 tag size = 32 INFO @ Wed, 28 Jun 2017 04:20:59: #1 total tags in treatment: 1701058 INFO @ Wed, 28 Jun 2017 04:20:59: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:20:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:20:59: #1 tag size is determined as 32 bps INFO @ Wed, 28 Jun 2017 04:20:59: #1 tag size = 32 INFO @ Wed, 28 Jun 2017 04:20:59: #1 total tags in treatment: 1701058 INFO @ Wed, 28 Jun 2017 04:20:59: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:20:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:20:59: #1 tags after filtering in treatment: 1701058 INFO @ Wed, 28 Jun 2017 04:20:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:20:59: #1 finished! INFO @ Wed, 28 Jun 2017 04:20:59: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:20:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:20:59: #1 tag size is determined as 32 bps INFO @ Wed, 28 Jun 2017 04:20:59: #1 tags after filtering in treatment: 1701058 INFO @ Wed, 28 Jun 2017 04:20:59: #1 tag size = 32 INFO @ Wed, 28 Jun 2017 04:20:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:20:59: #1 total tags in treatment: 1701058 INFO @ Wed, 28 Jun 2017 04:20:59: #1 finished! INFO @ Wed, 28 Jun 2017 04:20:59: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:20:59: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:20:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:20:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:20:59: #1 tags after filtering in treatment: 1701058 INFO @ Wed, 28 Jun 2017 04:20:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:20:59: #1 finished! INFO @ Wed, 28 Jun 2017 04:20:59: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:20:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:20:59: #2 number of paired peaks: 51 WARNING @ Wed, 28 Jun 2017 04:20:59: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:20:59: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 04:20:59: #2 number of paired peaks: 51 WARNING @ Wed, 28 Jun 2017 04:20:59: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:20:59: Process for pairing-model is terminated! cat: SRX092425.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX092425.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX092425.05_model.r'rm: cannot remove `SRX092425.20_model.r': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX092425.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX092425.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX092425.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX092425.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:20:59: #2 number of paired peaks: 51 WARNING @ Wed, 28 Jun 2017 04:20:59: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:20:59: Process for pairing-model is terminated! cat: SRX092425.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX092425.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX092425.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX092425.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。