Job ID = 9161698 sra ファイルのダウンロード中... Completed: 3977342K bytes transferred in 32 seconds (1001202K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 72666074 spots for /home/okishinya/chipatlas/results/sacCer3/SRX081806/SRR306545.sra Written 72666074 spots total Written 21148026 spots for /home/okishinya/chipatlas/results/sacCer3/SRX081806/SRR629574.sra Written 21148026 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:10 93814100 reads; of these: 93814100 (100.00%) were unpaired; of these: 25541235 (27.23%) aligned 0 times 61368212 (65.41%) aligned exactly 1 time 6904653 (7.36%) aligned >1 times 72.77% overall alignment rate Time searching: 00:20:10 Overall time: 00:20:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 56598815 / 68272865 = 0.8290 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 07:01:54: # Command line: callpeak -t SRX081806.bam -f BAM -g 12100000 -n SRX081806.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX081806.05 # format = BAM # ChIP-seq file = ['SRX081806.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:01:54: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:01:54: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:01:54: # Command line: callpeak -t SRX081806.bam -f BAM -g 12100000 -n SRX081806.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX081806.20 # format = BAM # ChIP-seq file = ['SRX081806.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:01:54: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:01:54: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:01:54: # Command line: callpeak -t SRX081806.bam -f BAM -g 12100000 -n SRX081806.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX081806.10 # format = BAM # ChIP-seq file = ['SRX081806.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:01:54: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:01:54: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:02:03: 1000000 INFO @ Wed, 28 Jun 2017 07:02:03: 1000000 INFO @ Wed, 28 Jun 2017 07:02:03: 1000000 INFO @ Wed, 28 Jun 2017 07:02:12: 2000000 INFO @ Wed, 28 Jun 2017 07:02:12: 2000000 INFO @ Wed, 28 Jun 2017 07:02:12: 2000000 INFO @ Wed, 28 Jun 2017 07:02:22: 3000000 INFO @ Wed, 28 Jun 2017 07:02:22: 3000000 INFO @ Wed, 28 Jun 2017 07:02:22: 3000000 INFO @ Wed, 28 Jun 2017 07:02:30: 4000000 INFO @ Wed, 28 Jun 2017 07:02:30: 4000000 INFO @ Wed, 28 Jun 2017 07:02:30: 4000000 INFO @ Wed, 28 Jun 2017 07:02:38: 5000000 INFO @ Wed, 28 Jun 2017 07:02:38: 5000000 INFO @ Wed, 28 Jun 2017 07:02:38: 5000000 INFO @ Wed, 28 Jun 2017 07:02:46: 6000000 INFO @ Wed, 28 Jun 2017 07:02:46: 6000000 INFO @ Wed, 28 Jun 2017 07:02:47: 6000000 INFO @ Wed, 28 Jun 2017 07:02:54: 7000000 INFO @ Wed, 28 Jun 2017 07:02:54: 7000000 INFO @ Wed, 28 Jun 2017 07:02:55: 7000000 INFO @ Wed, 28 Jun 2017 07:03:02: 8000000 INFO @ Wed, 28 Jun 2017 07:03:03: 8000000 INFO @ Wed, 28 Jun 2017 07:03:03: 8000000 INFO @ Wed, 28 Jun 2017 07:03:10: 9000000 INFO @ Wed, 28 Jun 2017 07:03:11: 9000000 INFO @ Wed, 28 Jun 2017 07:03:11: 9000000 INFO @ Wed, 28 Jun 2017 07:03:18: 10000000 INFO @ Wed, 28 Jun 2017 07:03:19: 10000000 INFO @ Wed, 28 Jun 2017 07:03:19: 10000000 INFO @ Wed, 28 Jun 2017 07:03:26: 11000000 INFO @ Wed, 28 Jun 2017 07:03:27: 11000000 INFO @ Wed, 28 Jun 2017 07:03:27: 11000000 INFO @ Wed, 28 Jun 2017 07:03:31: #1 tag size is determined as 70 bps INFO @ Wed, 28 Jun 2017 07:03:31: #1 tag size = 70 INFO @ Wed, 28 Jun 2017 07:03:31: #1 total tags in treatment: 11674050 INFO @ Wed, 28 Jun 2017 07:03:31: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:03:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:03:31: #1 tags after filtering in treatment: 11674050 INFO @ Wed, 28 Jun 2017 07:03:31: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:03:31: #1 finished! INFO @ Wed, 28 Jun 2017 07:03:31: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:03:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:03:32: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:03:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:03:32: Process for pairing-model is terminated! cat: SRX081806.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX081806.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX081806.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX081806.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:03:32: #1 tag size is determined as 70 bps INFO @ Wed, 28 Jun 2017 07:03:32: #1 tag size = 70 INFO @ Wed, 28 Jun 2017 07:03:32: #1 total tags in treatment: 11674050 INFO @ Wed, 28 Jun 2017 07:03:32: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:03:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:03:33: #1 tags after filtering in treatment: 11674050 INFO @ Wed, 28 Jun 2017 07:03:33: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:03:33: #1 finished! INFO @ Wed, 28 Jun 2017 07:03:33: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:03:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:03:33: #1 tag size is determined as 70 bps INFO @ Wed, 28 Jun 2017 07:03:33: #1 tag size = 70 INFO @ Wed, 28 Jun 2017 07:03:33: #1 total tags in treatment: 11674050 INFO @ Wed, 28 Jun 2017 07:03:33: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:03:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:03:33: #1 tags after filtering in treatment: 11674050 INFO @ Wed, 28 Jun 2017 07:03:33: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:03:33: #1 finished! INFO @ Wed, 28 Jun 2017 07:03:33: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:03:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:03:33: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:03:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:03:33: Process for pairing-model is terminated! cat: SRX081806.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX081806.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX081806.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX081806.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:03:34: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:03:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:03:34: Process for pairing-model is terminated! cat: SRX081806.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX081806.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX081806.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX081806.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。