Job ID = 9161680 sra ファイルのダウンロード中... Completed: 324948K bytes transferred in 6 seconds (440296K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 13033233 spots for /home/okishinya/chipatlas/results/sacCer3/SRX065609/SRR217312.sra Written 13033233 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:14 13033233 reads; of these: 13033233 (100.00%) were unpaired; of these: 4370594 (33.53%) aligned 0 times 1556795 (11.94%) aligned exactly 1 time 7105844 (54.52%) aligned >1 times 66.47% overall alignment rate Time searching: 00:01:14 Overall time: 00:01:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 7989200 / 8662639 = 0.9223 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:20:42: # Command line: callpeak -t SRX065609.bam -f BAM -g 12100000 -n SRX065609.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX065609.10 # format = BAM # ChIP-seq file = ['SRX065609.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:20:42: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:20:42: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:20:42: # Command line: callpeak -t SRX065609.bam -f BAM -g 12100000 -n SRX065609.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX065609.20 # format = BAM # ChIP-seq file = ['SRX065609.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:20:42: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:20:42: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:20:42: # Command line: callpeak -t SRX065609.bam -f BAM -g 12100000 -n SRX065609.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX065609.05 # format = BAM # ChIP-seq file = ['SRX065609.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:20:42: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:20:42: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:20:46: #1 tag size is determined as 36 bps INFO @ Wed, 28 Jun 2017 04:20:46: #1 tag size = 36 INFO @ Wed, 28 Jun 2017 04:20:46: #1 total tags in treatment: 673439 INFO @ Wed, 28 Jun 2017 04:20:46: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:20:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:20:46: #1 tags after filtering in treatment: 673439 INFO @ Wed, 28 Jun 2017 04:20:46: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:20:46: #1 finished! INFO @ Wed, 28 Jun 2017 04:20:46: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:20:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:20:46: #2 number of paired peaks: 65 WARNING @ Wed, 28 Jun 2017 04:20:46: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:20:46: Process for pairing-model is terminated! cat: SRX065609.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Wed, 28 Jun 2017 04:20:46: #1 tag size is determined as 36 bps INFO @ Wed, 28 Jun 2017 04:20:46: #1 tag size = 36 INFO @ Wed, 28 Jun 2017 04:20:46: #1 total tags in treatment: 673439 INFO @ Wed, 28 Jun 2017 04:20:46: #1 tag size is determined as 36 bps INFO @ Wed, 28 Jun 2017 04:20:46: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:20:46: #1 tag size = 36 INFO @ Wed, 28 Jun 2017 04:20:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:20:46: #1 total tags in treatment: 673439 INFO @ Wed, 28 Jun 2017 04:20:46: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:20:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX065609.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065609.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065609.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:20:46: #1 tags after filtering in treatment: 673439 INFO @ Wed, 28 Jun 2017 04:20:46: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:20:46: #1 finished! INFO @ Wed, 28 Jun 2017 04:20:46: #1 tags after filtering in treatment: 673439 INFO @ Wed, 28 Jun 2017 04:20:46: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:20:46: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:20:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:20:46: #1 finished! INFO @ Wed, 28 Jun 2017 04:20:46: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:20:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:20:46: #2 number of paired peaks: 65 WARNING @ Wed, 28 Jun 2017 04:20:46: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:20:46: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 04:20:46: #2 number of paired peaks: 65 WARNING @ Wed, 28 Jun 2017 04:20:46: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:20:46: Process for pairing-model is terminated! cat: SRX065609.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX065609.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX065609.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065609.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065609.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling rm: cannot remove `SRX065609.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065609.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065609.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。