Job ID = 9161678 sra ファイルのダウンロード中... Completed: 161994K bytes transferred in 4 seconds (289653K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 3200152 spots for /home/okishinya/chipatlas/results/sacCer3/SRX065605/SRR217307.sra Written 3200152 spots total Written 3382082 spots for /home/okishinya/chipatlas/results/sacCer3/SRX065605/SRR217308.sra Written 3382082 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:52 6582234 reads; of these: 6582234 (100.00%) were unpaired; of these: 43169 (0.66%) aligned 0 times 6411396 (97.40%) aligned exactly 1 time 127669 (1.94%) aligned >1 times 99.34% overall alignment rate Time searching: 00:00:52 Overall time: 00:00:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3011498 / 6539065 = 0.4605 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:19:22: # Command line: callpeak -t SRX065605.bam -f BAM -g 12100000 -n SRX065605.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX065605.10 # format = BAM # ChIP-seq file = ['SRX065605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:19:22: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:19:22: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:19:22: # Command line: callpeak -t SRX065605.bam -f BAM -g 12100000 -n SRX065605.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX065605.20 # format = BAM # ChIP-seq file = ['SRX065605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:19:22: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:19:22: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:19:22: # Command line: callpeak -t SRX065605.bam -f BAM -g 12100000 -n SRX065605.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX065605.05 # format = BAM # ChIP-seq file = ['SRX065605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:19:22: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:19:22: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:19:27: 1000000 INFO @ Wed, 28 Jun 2017 04:19:28: 1000000 INFO @ Wed, 28 Jun 2017 04:19:28: 1000000 INFO @ Wed, 28 Jun 2017 04:19:33: 2000000 INFO @ Wed, 28 Jun 2017 04:19:35: 2000000 INFO @ Wed, 28 Jun 2017 04:19:35: 2000000 INFO @ Wed, 28 Jun 2017 04:19:38: 3000000 INFO @ Wed, 28 Jun 2017 04:19:41: 3000000 INFO @ Wed, 28 Jun 2017 04:19:41: 3000000 INFO @ Wed, 28 Jun 2017 04:19:41: #1 tag size is determined as 36 bps INFO @ Wed, 28 Jun 2017 04:19:41: #1 tag size = 36 INFO @ Wed, 28 Jun 2017 04:19:41: #1 total tags in treatment: 3527567 INFO @ Wed, 28 Jun 2017 04:19:41: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:19:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:19:41: #1 tags after filtering in treatment: 3527567 INFO @ Wed, 28 Jun 2017 04:19:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:19:41: #1 finished! INFO @ Wed, 28 Jun 2017 04:19:41: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:19:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:19:42: #2 number of paired peaks: 5 WARNING @ Wed, 28 Jun 2017 04:19:42: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:19:42: Process for pairing-model is terminated! cat: SRX065605.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX065605.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065605.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065605.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:19:44: #1 tag size is determined as 36 bps INFO @ Wed, 28 Jun 2017 04:19:44: #1 tag size = 36 INFO @ Wed, 28 Jun 2017 04:19:44: #1 total tags in treatment: 3527567 INFO @ Wed, 28 Jun 2017 04:19:44: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:19:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:19:44: #1 tags after filtering in treatment: 3527567 INFO @ Wed, 28 Jun 2017 04:19:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:19:44: #1 finished! INFO @ Wed, 28 Jun 2017 04:19:44: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:19:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:19:44: #2 number of paired peaks: 5 WARNING @ Wed, 28 Jun 2017 04:19:44: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:19:44: Process for pairing-model is terminated! cat: SRX065605.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX065605.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065605.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065605.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:19:44: #1 tag size is determined as 36 bps INFO @ Wed, 28 Jun 2017 04:19:44: #1 tag size = 36 INFO @ Wed, 28 Jun 2017 04:19:44: #1 total tags in treatment: 3527567 INFO @ Wed, 28 Jun 2017 04:19:44: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:19:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:19:44: #1 tags after filtering in treatment: 3527567 INFO @ Wed, 28 Jun 2017 04:19:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:19:44: #1 finished! INFO @ Wed, 28 Jun 2017 04:19:44: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:19:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:19:44: #2 number of paired peaks: 5 WARNING @ Wed, 28 Jun 2017 04:19:44: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:19:44: Process for pairing-model is terminated! cat: SRX065605.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX065605.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065605.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX065605.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。