Job ID = 9161667 sra ファイルのダウンロード中... Completed: 1166282K bytes transferred in 15 seconds (598940K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 27347504 spots for /home/okishinya/chipatlas/results/sacCer3/SRX062021/SRR202046.sra Written 27347504 spots total Written 27650037 spots for /home/okishinya/chipatlas/results/sacCer3/SRX062021/SRR202045.sra Written 27650037 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:26 54997541 reads; of these: 54997541 (100.00%) were unpaired; of these: 15622229 (28.41%) aligned 0 times 19757325 (35.92%) aligned exactly 1 time 19617987 (35.67%) aligned >1 times 71.59% overall alignment rate Time searching: 00:06:26 Overall time: 00:06:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 34381859 / 39375312 = 0.8732 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:29:43: # Command line: callpeak -t SRX062021.bam -f BAM -g 12100000 -n SRX062021.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX062021.05 # format = BAM # ChIP-seq file = ['SRX062021.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:29:43: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:29:43: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:29:43: # Command line: callpeak -t SRX062021.bam -f BAM -g 12100000 -n SRX062021.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX062021.20 # format = BAM # ChIP-seq file = ['SRX062021.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:29:43: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:29:43: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:29:43: # Command line: callpeak -t SRX062021.bam -f BAM -g 12100000 -n SRX062021.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX062021.10 # format = BAM # ChIP-seq file = ['SRX062021.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:29:43: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:29:43: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:29:52: 1000000 INFO @ Wed, 28 Jun 2017 04:29:52: 1000000 INFO @ Wed, 28 Jun 2017 04:29:52: 1000000 INFO @ Wed, 28 Jun 2017 04:29:59: 2000000 INFO @ Wed, 28 Jun 2017 04:29:59: 2000000 INFO @ Wed, 28 Jun 2017 04:30:00: 2000000 INFO @ Wed, 28 Jun 2017 04:30:06: 3000000 INFO @ Wed, 28 Jun 2017 04:30:06: 3000000 INFO @ Wed, 28 Jun 2017 04:30:08: 3000000 INFO @ Wed, 28 Jun 2017 04:30:13: 4000000 INFO @ Wed, 28 Jun 2017 04:30:14: 4000000 INFO @ Wed, 28 Jun 2017 04:30:15: 4000000 INFO @ Wed, 28 Jun 2017 04:30:20: #1 tag size is determined as 38 bps INFO @ Wed, 28 Jun 2017 04:30:20: #1 tag size = 38 INFO @ Wed, 28 Jun 2017 04:30:20: #1 total tags in treatment: 4993453 INFO @ Wed, 28 Jun 2017 04:30:20: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:30:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:30:20: #1 tags after filtering in treatment: 4993453 INFO @ Wed, 28 Jun 2017 04:30:20: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:30:20: #1 finished! INFO @ Wed, 28 Jun 2017 04:30:20: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:30:20: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:30:20: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:30:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:30:20: Process for pairing-model is terminated! cat: SRX062021.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX062021.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062021.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062021.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:30:21: #1 tag size is determined as 38 bps INFO @ Wed, 28 Jun 2017 04:30:21: #1 tag size = 38 INFO @ Wed, 28 Jun 2017 04:30:21: #1 total tags in treatment: 4993453 INFO @ Wed, 28 Jun 2017 04:30:21: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:30:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:30:21: #1 tags after filtering in treatment: 4993453 INFO @ Wed, 28 Jun 2017 04:30:21: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:30:21: #1 finished! INFO @ Wed, 28 Jun 2017 04:30:21: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:30:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:30:21: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:30:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:30:21: Process for pairing-model is terminated! cat: SRX062021.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX062021.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062021.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062021.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:30:22: #1 tag size is determined as 38 bps INFO @ Wed, 28 Jun 2017 04:30:22: #1 tag size = 38 INFO @ Wed, 28 Jun 2017 04:30:22: #1 total tags in treatment: 4993453 INFO @ Wed, 28 Jun 2017 04:30:22: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:30:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:30:22: #1 tags after filtering in treatment: 4993453 INFO @ Wed, 28 Jun 2017 04:30:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:30:22: #1 finished! INFO @ Wed, 28 Jun 2017 04:30:22: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:30:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:30:23: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:30:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:30:23: Process for pairing-model is terminated! cat: SRX062021.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX062021.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062021.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX062021.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。