Job ID = 9161665 sra ファイルのダウンロード中... Completed: 763321K bytes transferred in 13 seconds (464945K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 31046728 spots for /home/okishinya/chipatlas/results/sacCer3/SRX058455/SRR189600.sra Written 31046728 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:11 31046728 reads; of these: 31046728 (100.00%) were unpaired; of these: 1196165 (3.85%) aligned 0 times 25856698 (83.28%) aligned exactly 1 time 3993865 (12.86%) aligned >1 times 96.15% overall alignment rate Time searching: 00:05:11 Overall time: 00:05:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 15576194 / 29850563 = 0.5218 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:28:26: # Command line: callpeak -t SRX058455.bam -f BAM -g 12100000 -n SRX058455.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX058455.10 # format = BAM # ChIP-seq file = ['SRX058455.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:28:26: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:28:26: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:28:26: # Command line: callpeak -t SRX058455.bam -f BAM -g 12100000 -n SRX058455.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX058455.05 # format = BAM # ChIP-seq file = ['SRX058455.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:28:26: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:28:26: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:28:26: # Command line: callpeak -t SRX058455.bam -f BAM -g 12100000 -n SRX058455.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX058455.20 # format = BAM # ChIP-seq file = ['SRX058455.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:28:26: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:28:26: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:28:32: 1000000 INFO @ Wed, 28 Jun 2017 04:28:32: 1000000 INFO @ Wed, 28 Jun 2017 04:28:32: 1000000 INFO @ Wed, 28 Jun 2017 04:28:38: 2000000 INFO @ Wed, 28 Jun 2017 04:28:39: 2000000 INFO @ Wed, 28 Jun 2017 04:28:39: 2000000 INFO @ Wed, 28 Jun 2017 04:28:44: 3000000 INFO @ Wed, 28 Jun 2017 04:28:45: 3000000 INFO @ Wed, 28 Jun 2017 04:28:45: 3000000 INFO @ Wed, 28 Jun 2017 04:28:50: 4000000 INFO @ Wed, 28 Jun 2017 04:28:51: 4000000 INFO @ Wed, 28 Jun 2017 04:28:51: 4000000 INFO @ Wed, 28 Jun 2017 04:28:57: 5000000 INFO @ Wed, 28 Jun 2017 04:28:58: 5000000 INFO @ Wed, 28 Jun 2017 04:28:58: 5000000 INFO @ Wed, 28 Jun 2017 04:29:03: 6000000 INFO @ Wed, 28 Jun 2017 04:29:04: 6000000 INFO @ Wed, 28 Jun 2017 04:29:04: 6000000 INFO @ Wed, 28 Jun 2017 04:29:09: 7000000 INFO @ Wed, 28 Jun 2017 04:29:10: 7000000 INFO @ Wed, 28 Jun 2017 04:29:10: 7000000 INFO @ Wed, 28 Jun 2017 04:29:15: 8000000 INFO @ Wed, 28 Jun 2017 04:29:16: 8000000 INFO @ Wed, 28 Jun 2017 04:29:16: 8000000 INFO @ Wed, 28 Jun 2017 04:29:21: 9000000 INFO @ Wed, 28 Jun 2017 04:29:23: 9000000 INFO @ Wed, 28 Jun 2017 04:29:23: 9000000 INFO @ Wed, 28 Jun 2017 04:29:27: 10000000 INFO @ Wed, 28 Jun 2017 04:29:30: 10000000 INFO @ Wed, 28 Jun 2017 04:29:30: 10000000 INFO @ Wed, 28 Jun 2017 04:29:33: 11000000 INFO @ Wed, 28 Jun 2017 04:29:36: 11000000 INFO @ Wed, 28 Jun 2017 04:29:36: 11000000 INFO @ Wed, 28 Jun 2017 04:29:40: 12000000 INFO @ Wed, 28 Jun 2017 04:29:42: 12000000 INFO @ Wed, 28 Jun 2017 04:29:42: 12000000 INFO @ Wed, 28 Jun 2017 04:29:46: 13000000 INFO @ Wed, 28 Jun 2017 04:29:49: 13000000 INFO @ Wed, 28 Jun 2017 04:29:49: 13000000 INFO @ Wed, 28 Jun 2017 04:29:52: 14000000 INFO @ Wed, 28 Jun 2017 04:29:53: #1 tag size is determined as 40 bps INFO @ Wed, 28 Jun 2017 04:29:53: #1 tag size = 40 INFO @ Wed, 28 Jun 2017 04:29:53: #1 total tags in treatment: 14274369 INFO @ Wed, 28 Jun 2017 04:29:53: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:29:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:29:54: #1 tags after filtering in treatment: 14274369 INFO @ Wed, 28 Jun 2017 04:29:54: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:29:54: #1 finished! INFO @ Wed, 28 Jun 2017 04:29:54: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:29:54: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:29:55: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:29:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:29:55: Process for pairing-model is terminated! cat: SRX058455.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX058455.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX058455.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX058455.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:29:55: 14000000 INFO @ Wed, 28 Jun 2017 04:29:55: 14000000 INFO @ Wed, 28 Jun 2017 04:29:57: #1 tag size is determined as 40 bps INFO @ Wed, 28 Jun 2017 04:29:57: #1 tag size = 40 INFO @ Wed, 28 Jun 2017 04:29:57: #1 total tags in treatment: 14274369 INFO @ Wed, 28 Jun 2017 04:29:57: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:29:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:29:57: #1 tag size is determined as 40 bps INFO @ Wed, 28 Jun 2017 04:29:57: #1 tag size = 40 INFO @ Wed, 28 Jun 2017 04:29:57: #1 total tags in treatment: 14274369 INFO @ Wed, 28 Jun 2017 04:29:57: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:29:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:29:57: #1 tags after filtering in treatment: 14274369 INFO @ Wed, 28 Jun 2017 04:29:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:29:57: #1 finished! INFO @ Wed, 28 Jun 2017 04:29:57: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:29:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:29:57: #1 tags after filtering in treatment: 14274369 INFO @ Wed, 28 Jun 2017 04:29:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:29:57: #1 finished! INFO @ Wed, 28 Jun 2017 04:29:57: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:29:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:29:58: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:29:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:29:58: Process for pairing-model is terminated! cat: SRX058455.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX058455.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX058455.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX058455.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:29:58: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:29:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:29:58: Process for pairing-model is terminated! cat: SRX058455.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX058455.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX058455.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX058455.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。