Job ID = 2009652 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,205,065 reads read : 3,205,065 reads written : 3,205,065 spots read : 4,000,000 reads read : 4,000,000 reads written : 4,000,000 spots read : 4,000,000 reads read : 4,000,000 reads written : 4,000,000 spots read : 4,000,000 reads read : 4,000,000 reads written : 4,000,000 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778530.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778542.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778676.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:27 15205065 reads; of these: 15205065 (100.00%) were unpaired; of these: 2917166 (19.19%) aligned 0 times 11588775 (76.22%) aligned exactly 1 time 699124 (4.60%) aligned >1 times 80.81% overall alignment rate Time searching: 00:02:27 Overall time: 00:02:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8073567 / 12287899 = 0.6570 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:38:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:38:54: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:38:54: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:38:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:38:55: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:38:55: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:38:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:38:56: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:38:56: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:39:03: 1000000 INFO @ Fri, 05 Jul 2019 19:39:04: 1000000 INFO @ Fri, 05 Jul 2019 19:39:05: 1000000 INFO @ Fri, 05 Jul 2019 19:39:11: 2000000 INFO @ Fri, 05 Jul 2019 19:39:12: 2000000 INFO @ Fri, 05 Jul 2019 19:39:13: 2000000 INFO @ Fri, 05 Jul 2019 19:39:20: 3000000 INFO @ Fri, 05 Jul 2019 19:39:20: 3000000 INFO @ Fri, 05 Jul 2019 19:39:21: 3000000 INFO @ Fri, 05 Jul 2019 19:39:27: 4000000 INFO @ Fri, 05 Jul 2019 19:39:28: 4000000 INFO @ Fri, 05 Jul 2019 19:39:29: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 19:39:29: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 19:39:29: #1 total tags in treatment: 4214332 INFO @ Fri, 05 Jul 2019 19:39:29: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:39:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:39:29: #1 tags after filtering in treatment: 4214332 INFO @ Fri, 05 Jul 2019 19:39:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:39:29: #1 finished! INFO @ Fri, 05 Jul 2019 19:39:29: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:39:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:39:29: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:39:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:39:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:39:30: 4000000 INFO @ Fri, 05 Jul 2019 19:39:30: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 19:39:30: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 19:39:30: #1 total tags in treatment: 4214332 INFO @ Fri, 05 Jul 2019 19:39:30: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:39:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:39:30: #1 tags after filtering in treatment: 4214332 INFO @ Fri, 05 Jul 2019 19:39:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:39:30: #1 finished! INFO @ Fri, 05 Jul 2019 19:39:30: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:39:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:39:30: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:39:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:39:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:39:31: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 19:39:31: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 19:39:31: #1 total tags in treatment: 4214332 INFO @ Fri, 05 Jul 2019 19:39:31: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:39:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:39:31: #1 tags after filtering in treatment: 4214332 INFO @ Fri, 05 Jul 2019 19:39:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:39:31: #1 finished! INFO @ Fri, 05 Jul 2019 19:39:31: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:39:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:39:32: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:39:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:39:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769147/ERX769147.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。