Job ID = 2009642 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,000,000 reads read : 4,000,000 reads written : 4,000,000 spots read : 4,000,000 reads read : 4,000,000 reads written : 4,000,000 spots read : 1,096,171 reads read : 1,096,171 reads written : 1,096,171 spots read : 4,000,000 reads read : 4,000,000 reads written : 4,000,000 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778611.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778619.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778633.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:08 13096171 reads; of these: 13096171 (100.00%) were unpaired; of these: 2354919 (17.98%) aligned 0 times 10096154 (77.09%) aligned exactly 1 time 645098 (4.93%) aligned >1 times 82.02% overall alignment rate Time searching: 00:02:08 Overall time: 00:02:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6164642 / 10741252 = 0.5739 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:29:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:29:42: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:29:42: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:29:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:29:43: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:29:43: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:29:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:29:44: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:29:44: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:29:51: 1000000 INFO @ Fri, 05 Jul 2019 19:29:52: 1000000 INFO @ Fri, 05 Jul 2019 19:29:53: 1000000 INFO @ Fri, 05 Jul 2019 19:30:01: 2000000 INFO @ Fri, 05 Jul 2019 19:30:02: 2000000 INFO @ Fri, 05 Jul 2019 19:30:03: 2000000 INFO @ Fri, 05 Jul 2019 19:30:10: 3000000 INFO @ Fri, 05 Jul 2019 19:30:11: 3000000 INFO @ Fri, 05 Jul 2019 19:30:13: 3000000 INFO @ Fri, 05 Jul 2019 19:30:20: 4000000 INFO @ Fri, 05 Jul 2019 19:30:20: 4000000 INFO @ Fri, 05 Jul 2019 19:30:22: 4000000 INFO @ Fri, 05 Jul 2019 19:30:25: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 19:30:25: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 19:30:25: #1 total tags in treatment: 4576610 INFO @ Fri, 05 Jul 2019 19:30:25: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:30:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:30:25: #1 tags after filtering in treatment: 4576610 INFO @ Fri, 05 Jul 2019 19:30:25: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:30:25: #1 finished! INFO @ Fri, 05 Jul 2019 19:30:25: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:30:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:30:25: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:30:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:30:25: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:30:25: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 19:30:25: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 19:30:25: #1 total tags in treatment: 4576610 INFO @ Fri, 05 Jul 2019 19:30:25: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:30:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:30:25: #1 tags after filtering in treatment: 4576610 INFO @ Fri, 05 Jul 2019 19:30:25: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:30:25: #1 finished! INFO @ Fri, 05 Jul 2019 19:30:25: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:30:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:30:26: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:30:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:30:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.05_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.10_model.r’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:30:27: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 19:30:27: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 19:30:27: #1 total tags in treatment: 4576610 INFO @ Fri, 05 Jul 2019 19:30:27: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:30:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:30:27: #1 tags after filtering in treatment: 4576610 INFO @ Fri, 05 Jul 2019 19:30:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:30:27: #1 finished! INFO @ Fri, 05 Jul 2019 19:30:27: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:30:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:30:27: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:30:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:30:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769123/ERX769123.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。