Job ID = 2009633 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,000,000 reads read : 4,000,000 reads written : 4,000,000 spots read : 4,000,000 reads read : 4,000,000 reads written : 4,000,000 spots read : 1,784,738 reads read : 1,784,738 reads written : 1,784,738 spots read : 4,000,000 reads read : 4,000,000 reads written : 4,000,000 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778515.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778613.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778714.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:20 13784738 reads; of these: 13784738 (100.00%) were unpaired; of these: 2911508 (21.12%) aligned 0 times 10338418 (75.00%) aligned exactly 1 time 534812 (3.88%) aligned >1 times 78.88% overall alignment rate Time searching: 00:08:20 Overall time: 00:08:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6163829 / 10873230 = 0.5669 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:38:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:38:56: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:38:56: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:38:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:38:57: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:38:57: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:38:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:38:58: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:38:58: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:39:05: 1000000 INFO @ Fri, 05 Jul 2019 19:39:08: 1000000 INFO @ Fri, 05 Jul 2019 19:39:09: 1000000 INFO @ Fri, 05 Jul 2019 19:39:15: 2000000 INFO @ Fri, 05 Jul 2019 19:39:17: 2000000 INFO @ Fri, 05 Jul 2019 19:39:20: 2000000 INFO @ Fri, 05 Jul 2019 19:39:24: 3000000 INFO @ Fri, 05 Jul 2019 19:39:26: 3000000 INFO @ Fri, 05 Jul 2019 19:39:32: 3000000 INFO @ Fri, 05 Jul 2019 19:39:33: 4000000 INFO @ Fri, 05 Jul 2019 19:39:35: 4000000 INFO @ Fri, 05 Jul 2019 19:39:39: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 19:39:39: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 19:39:39: #1 total tags in treatment: 4709401 INFO @ Fri, 05 Jul 2019 19:39:39: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:39:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:39:39: #1 tags after filtering in treatment: 4709401 INFO @ Fri, 05 Jul 2019 19:39:39: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:39:39: #1 finished! INFO @ Fri, 05 Jul 2019 19:39:39: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:39:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:39:39: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:39:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:39:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:39:42: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 19:39:42: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 19:39:42: #1 total tags in treatment: 4709401 INFO @ Fri, 05 Jul 2019 19:39:42: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:39:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:39:42: #1 tags after filtering in treatment: 4709401 INFO @ Fri, 05 Jul 2019 19:39:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:39:42: #1 finished! INFO @ Fri, 05 Jul 2019 19:39:42: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:39:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:39:42: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:39:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:39:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:39:42: 4000000 INFO @ Fri, 05 Jul 2019 19:39:50: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 19:39:50: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 19:39:50: #1 total tags in treatment: 4709401 INFO @ Fri, 05 Jul 2019 19:39:50: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:39:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:39:50: #1 tags after filtering in treatment: 4709401 INFO @ Fri, 05 Jul 2019 19:39:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:39:50: #1 finished! INFO @ Fri, 05 Jul 2019 19:39:50: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:39:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:39:50: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:39:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:39:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769110/ERX769110.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。