Job ID = 2009632 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,000,000 reads read : 5,000,000 reads written : 5,000,000 spots read : 5,000,000 reads read : 5,000,000 reads written : 5,000,000 spots read : 5,000,000 reads read : 5,000,000 reads written : 5,000,000 spots read : 5,000,000 reads read : 5,000,000 reads written : 5,000,000 spots read : 5,000,000 reads read : 5,000,000 reads written : 5,000,000 spots read : 876,476 reads read : 876,476 reads written : 876,476 spots read : 5,000,000 reads read : 5,000,000 reads written : 5,000,000 spots read : 5,000,000 reads read : 5,000,000 reads written : 5,000,000 spots read : 5,000,000 reads read : 5,000,000 reads written : 5,000,000 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778539.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778552.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778582.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR778608.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:23 40876476 reads; of these: 40876476 (100.00%) were unpaired; of these: 3991281 (9.76%) aligned 0 times 33937268 (83.02%) aligned exactly 1 time 2947927 (7.21%) aligned >1 times 90.24% overall alignment rate Time searching: 00:07:23 Overall time: 00:07:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 28669420 / 36885195 = 0.7773 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:30:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:30:21: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:30:21: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:30:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:30:22: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:30:22: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:30:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:30:23: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:30:23: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:30:30: 1000000 INFO @ Fri, 05 Jul 2019 20:30:31: 1000000 INFO @ Fri, 05 Jul 2019 20:30:33: 1000000 INFO @ Fri, 05 Jul 2019 20:30:40: 2000000 INFO @ Fri, 05 Jul 2019 20:30:41: 2000000 INFO @ Fri, 05 Jul 2019 20:30:43: 2000000 INFO @ Fri, 05 Jul 2019 20:30:50: 3000000 INFO @ Fri, 05 Jul 2019 20:30:50: 3000000 INFO @ Fri, 05 Jul 2019 20:30:53: 3000000 INFO @ Fri, 05 Jul 2019 20:31:00: 4000000 INFO @ Fri, 05 Jul 2019 20:31:00: 4000000 INFO @ Fri, 05 Jul 2019 20:31:02: 4000000 INFO @ Fri, 05 Jul 2019 20:31:09: 5000000 INFO @ Fri, 05 Jul 2019 20:31:10: 5000000 INFO @ Fri, 05 Jul 2019 20:31:12: 5000000 INFO @ Fri, 05 Jul 2019 20:31:19: 6000000 INFO @ Fri, 05 Jul 2019 20:31:19: 6000000 INFO @ Fri, 05 Jul 2019 20:31:22: 6000000 INFO @ Fri, 05 Jul 2019 20:31:29: 7000000 INFO @ Fri, 05 Jul 2019 20:31:29: 7000000 INFO @ Fri, 05 Jul 2019 20:31:31: 7000000 INFO @ Fri, 05 Jul 2019 20:31:37: 8000000 INFO @ Fri, 05 Jul 2019 20:31:38: 8000000 INFO @ Fri, 05 Jul 2019 20:31:38: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 20:31:38: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 20:31:38: #1 total tags in treatment: 8215775 INFO @ Fri, 05 Jul 2019 20:31:38: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:31:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:31:38: 8000000 INFO @ Fri, 05 Jul 2019 20:31:39: #1 tags after filtering in treatment: 8215775 INFO @ Fri, 05 Jul 2019 20:31:39: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:31:39: #1 finished! INFO @ Fri, 05 Jul 2019 20:31:39: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:31:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:31:39: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:31:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:31:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:31:39: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 20:31:39: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 20:31:39: #1 total tags in treatment: 8215775 INFO @ Fri, 05 Jul 2019 20:31:39: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:31:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:31:40: #1 tags after filtering in treatment: 8215775 INFO @ Fri, 05 Jul 2019 20:31:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:31:40: #1 finished! INFO @ Fri, 05 Jul 2019 20:31:40: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:31:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:31:40: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 20:31:40: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 20:31:40: #1 total tags in treatment: 8215775 INFO @ Fri, 05 Jul 2019 20:31:40: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:31:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:31:40: #1 tags after filtering in treatment: 8215775 INFO @ Fri, 05 Jul 2019 20:31:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:31:40: #1 finished! INFO @ Fri, 05 Jul 2019 20:31:40: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:31:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:31:40: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:31:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:31:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:31:41: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:31:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:31:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX769107/ERX769107.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。